An improved screw-free method for electrode implantation and intracranial electroencephalographic recordings in mice |
| |
Authors: | Chiping Wu Marta Wais Tariq Zahid Qi Wan Liang Zhang |
| |
Institution: | (1) Westmead Hospital, Darcy Rd, Westmead, New South Wales, 2145, Australia;(2) The Prince of Wales Hospital, High St, Randwick, New South Wales, 2031, Australia;(3) The Children’s Hospital at Westmead, Hawkesbury Rd, Westmead, New South Wales, 2145, Australia;(4) Epilepsy Unit, Department of Neurology, Westmead Hospital, P.O. Box 533, Wentworthville, NSW, 2145, Australia; |
| |
Abstract: | We recently developed a glue-based method for the implantation of intracranial electrodes in mice. Our approach is to secure
a preconstructed electrode array using a cyanoacrylate-based glue (similar to Krazy Glue). This method is applicable to both
young and aging mice and is suitable for long-term electroencephalographic recordings. In the present experiment, we explored
whether the glue-based method is capable of securing individual electrodes in addition to securing the electrode array. C57
black mice aged 25–35 days or 13–19 months were operated on under isoflurane anesthesia. Monopolar or bipolar electrodes were
inserted independently in the ipsilateral hippocampal CA3 and entorhinal cortical areas, and they were fixed onto the skull
using the glue together with dental acrylic, but without anchoring screws. We found that the implanted electrodes were stable
and allowed repeat intracranial recordings and electrical stimulation in freely moving mice. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|