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Methods for rapid quantification of brain autoradiographs
Authors:J Michael Walker  David G Lavond  David J Bodart  Michael S Beattie  Patricia M Meyer
Institution:1. Laboratory of Comparative and Physiological Psychology, Ohio State University, 43212, Columbus, Ohio
Abstract:Apparatus and methods are described for rapid semiautomatic quantification of silver grain densities in brain autoradiographs. The output voltage of a photodiode used with dark-field oil microscopy reflects the amount of label in a 35 by 35 square micra area of brain tissue. Apparatus are described for monitoring the movement of a microscope stage, and a microprocessor interface is described that provides hard-copy and paper-tape output of grain densities for later processing.
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