Methods for rapid quantification of brain autoradiographs |
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Authors: | J Michael Walker David G Lavond David J Bodart Michael S Beattie Patricia M Meyer |
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Institution: | 1. Laboratory of Comparative and Physiological Psychology, Ohio State University, 43212, Columbus, Ohio
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Abstract: | Apparatus and methods are described for rapid semiautomatic quantification of silver grain densities in brain autoradiographs. The output voltage of a photodiode used with dark-field oil microscopy reflects the amount of label in a 35 by 35 square micra area of brain tissue. Apparatus are described for monitoring the movement of a microscope stage, and a microprocessor interface is described that provides hard-copy and paper-tape output of grain densities for later processing. |
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