A method of restraining goldfish during conditioning of electric-shock-induced suppression of breathing movements

An efficient method of holding individual fish in position for delivery of visual conditioned stimuli and electrical unconditioned stimuli, and for registration of breathing movements with a thermistor, is described. The holder restrains the fish gently and is readily opened and closed. In addition, a “mechanical fish” signal generator, for assessing thermistor performance in detecting breathing movements, and a safe constant-current electrical shock stimulus generator are described.