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Quantitative chromatographic profiling of odours associated with dominance in male laboratory mice
Authors:P. J. Apps  A. Rasa  H. W. Viljoen
Abstract:Triads of male NMRI mice were housed together and dominance hierarchies allowed to form. At age 19 weeks the mice were ranked on the basis of wins in spontaneous aggressive encounters, and whole-body volatiles were sampled by the dynamic solvent effect and quantitatively analysed by capillary gas chromatography. At age 26 weeks the mice were again ranked on the basis of wins in aggressive encounters, number of aggressive encounters initiated, and scent marking patterns, and whole-body volatiles were sampled and analysed. Chromatographic odour profile ranks agreed perfectly with aggressive initiator ranks and poorly with encounter winner ranks. Eight components of the whole-body odour were identified by gas chromatography-mass spectrometry; two of these are known semiochemicals. The results demonstrate that murine semiochemicals are accessible to quantitative analysis at the level of the individual.
Keywords:Mus  semiochemical  pheromone  dynamic solvent effect  aggression  NMRI mice
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