味觉厌恶性条件反射建立后脑内c-Fos的表达

以新异味觉刺激糖精水的摄入为条件刺激,以腹腔注射环磷酰胺(CY,免疫抑制剂)或氯化锂(LiCl)为非条件刺激,分别使大鼠建立味觉厌恶性条件反射.在条件刺激日,糖精水在学习组大鼠下列脑区中诱发出密集的Fos表达下丘脑、杏仁核、边缘皮质等,而非学习组在这些区域中却没有或只有少量表达.另外,在丘脑前背侧核、扣带回、下丘脑外侧核、穹隆下器、压部后颗粒皮质、视上核,CY组的Fos表达明显多于LiCl组;而在伏核、杏仁基底外侧核、腹外侧隔核,LiCl组的Fos表达明显多于CY组,这种差异可能是两种药物的不同药理性质所致.

C-Fos Induction in Response to Taste Stimuli Previously Paired with CY Or LiCl during Taste Aversion Learning

C-Fos immunohistochemistry was used to define rat brain regions activated during expression of a CTA which was established by intake of saccharin solution (CS) paired with i.p. injection of CY or LiC1. Re-exposure to saccharin induced dense c-Fos-like immunoreactivity (c-FLI) in hypothalamus, amygdala and limbic cortex et al. in both CY-paired and LiC1-paired groups, but this pattern of activation was not evident in saccharin controls or CY (LiC1)-unpaired groups. However there were several brain regions which showed some difference in c-FLI between CY-paired and LiC1-paired groups. CY-paired CS induced denser c-FLI than LiC1-paired CS in anterodorsal thalamic nucleus (AD), cingulate cortex (Cg), lateral hypothalamic area(LH), subfornical organ (SFD) and supraoptic nucleus(SO), retrosplenial granular cortex (RSG), these regions may be related to the action of immunosuppression induced by CY; But in accumbens nucleus (Acb), basotateral amydaloid nucleus(BL), ventral part of Latelal septal nucleus (LSV), LiC1-paired CS induced denser c-FLI than CY-paired CS, these regions may be related to the pharmacologic effects of LiC1 to the brain.