Partial purification of male mus musculus urinary aggression-promoting chemosignal

An attempt was made to purify an aggression-promoting chemosignal of male SJL/J mice. It was shown in Experiment 1 that the chemosignal is present in intact-male urine (IU) but not in urine (CU) from castrated males. In Experiments 3 and 4, a head-space distillation technique was used to isolate the volatile components of IU. It was shown that aggression-promoting cues were present in the volatile portion of IU and not in the nonvolatile portion. Also CU was tested for behavioral activity under the procedures of Experiments 3 and 4 and was found not to contain the chemosensory cue.