EGF对A431细胞中MMP-12表达的调节

基质金属蛋白酶（MMPs）和表皮生长因子（EGF）与肿瘤的发生和转移有密切关系，为了研究EGF对MMP-12的调节机制，我们用不同浓度的EGF处理人表皮癌细胞（A431细胞）后，观察MMP-12的表达变化。结果显示使用不同浓度EGF处理A431细胞后，MMP-12的mRNA和蛋白水平均有所升高。因此，在A431细胞中，EGF通过上调MMP-12的表达可能会促进肿瘤的侵袭和转移。

The Regulation of EGF on MMP--12 in A431 Cells

Matrix metalloproteinase（MMPs） and epidermal growth factor （EGF） are both have a close relationship with tumor＇ s genesis and metastasis. To investigate the effects of EGF on MMP-- 12 in human epidermoid carcinoma A431 ceils, we treated A431 cells with different concentration of EGF to observe the changes of MMP--12＇s expression. After the A431 cells treated with EGF, the mRNA and protein expression of MMP--12 were both increased. Along with the hoist of the EGF＇s concentration, the expression of MMP--12 was augment. In the process of tumor metastasis, EGF could regulate the expression of MMP--12 to promote the tumor＇s invasion. Therefore, the high expression of MMP--12 could accelerate the degradation of extracelluar matrix, and probably provide an proper condition for the tumor＇s transfer.