大鼠脑反复缺血致不可逆性学习记忆障碍的研究

大鼠全脑反复缺血后产生不可逆性学习记忆障碍的研究，采用４血管阻断的方法，脑反复缺血再灌流，然后长期饲养。作水迷宫试验和跳台试验，结果显示缺血后出现了显著的、不可逆的学习记忆障碍。同时用放射免疫方法测定了额叶、颞叶、纹状体、丘脑、海马、Ｍｅｙｎｅｒｔ氏核中胆碱递质和ＡＶＰ，发现ＡＣｈ、ＣｈＡＴ、ＡＶＰ含量显著下降。下降的时间、幅度一致，变化呈正相关。ＡＣｈ、ＣｈＡＴ、ＡＶＰ是与学习、记忆密切相关的神经递质和神经肽。因而推断脑缺血后出现不可逆性学习记忆障碍与多脑区内ＡＣｈ、ＣｈＡＴ、ＡＶＰ含量显著下降有关。这对探讨临床上血管性痴呆发病机理进而开发有效的防治药物有重要意义。

ISCHEMIC-INDUCED IRREVERSIBLE DEFICIT OF MEMORY FUNCTION IN WISTAR RATS

This paper reported that ischemia-induced irreversible deficit of memory function in Wistar Rats surviving for a long time after global ischemia by 4-vessel occlusion method in the Water Maze Test and Step Down Test.Contents of ACh, ChAT and AVP were declined in the six brain regions i.e.,frontal cortex,temporal cortex, striatum, hippocampus, thalamus and Myenert nucleus, Changes in the memory function and in the contents of transmitters showed no defference in time and extent. It was proved that ACh, ChAT and AVP were neurotransmitters and neuropeptide which are closely associated with learning and memory.It was also suggested that they play and important part in the process of learning and memory.So these results indicated that changes in the contents of ACh, ChAT and AVP in regions produced irreversible deficit of the memory function.This study is important for reseach mechanisms of vascular dementia and in search of efficient drugs.