| 真核双基因表达载体p1RES-Rb94-E1A的构建与鉴定 |
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| 引用本文： | 刘芳,李清宝,李萌,栾力,王建波,程玉峰. 真核双基因表达载体p1RES-Rb94-E1A的构建与鉴定[J]. 医学与哲学(人文社会医学版), 2011, 0(6): 59-61 |
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| 作者姓名： | 刘芳 李清宝 李萌 栾力 王建波 程玉峰 |
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| 作者单位： | [1]山东医学高等专科学校影像系,山东济南250002 [2]山东大学附属省立医院心脏外科,山东济南250021 [3]山东大学齐鲁医院放疗科,山东济南250012 |
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| 摘 要： | 以pE1A质粒为模板，扩增E1A基因，然后将E1AcDNA片段插入前期已经构建好的真核表达载体pIRES-Rb94的MCSB的SalI与NotI之间，获得真核双基因表达质粒p1RES-Rb94-E1A，转染肺腺癌A549细胞后，采用RT—PCR和Westernblot方法检测Rb94和E1A在A549细胞中的表达，为研...
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| 关 键 词： | 基因 表达载体 A549细胞 |
Construction and Identification of the Eukaryotic Co--expression Plasmid pIRES-- Rb94- E1A |
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| Affiliation: | LIU Fang, LI Qing-bao, LI Meng, et al. (Department of Radiation, Shandong Medical College, Jinan 250002, China ) |
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| Abstract: | The cDNA encoding E1A was obtained by RT--PCR from plasmid E1A, then inserted into the MCSB of the plasmid pIRES--Rb94 between SalI and NotL Plasmid pIRES--Rb94--E1A was constructed and A549 cells were trans- formed by plasmid pIRES--Rb94--E1A. RT--PCR and Western blot were used to identify the co--expression of Rb94 and E1A in A549 cells. The successful construction of plasmid pIRES--Rb94--E1A can establish an experimental basis and provide scientific foundation for clinical cancer treatment. |
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| Keywords: | gene expression vector A549 cells |
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