慢性乙型肝炎T细胞功能与HBV前C区变异的研究

通过研究慢性HBV感染者前C区变异及T细胞免疫功能与疾病的相关性，评估前C（preC）区变异及T细胞免疫功能对于慢性乙型肝炎（chronichepatitisB，CHB）预后的影响。分别采用实时荧光法、突变特异PCRmsPCR法及微粒酶免疫分析法对150例HBV-DNA阳性的CHB及50例健康体检者进行血清HBV-DN...

The Relationship between Function of T Cells Function and Pre- C Mutation in Chronic Hepatitis B Patients

To explore the effect of the function of cellular immunity and Pre--c mutation on the prognosis in patients with chronic hepatitis B. Total 150 cases of CHB patients were included in the study. Pre--c mutation was analyzed by msPCR. HBV--DNA levels were measured by real time PCR. The levels of CD4 and CD8 ＋ T cells were measured by APAAP method. The rate of Pre -- c mutation was 26. 6 % （ 40/150 ） in CHB patients. G1896A mutation was largely detected in HBeAg negative CHB patients. The levels of CD4 and ratios of CD4/CD8 were decreased significantly in both variant and wild-type patients, whereas CD8 was increased significantly, compared with those in control. The levels of CD4 and ratios of CD4/CD8 in variant group were lower than wild--type group, but the level of CD8 was higher in variant group. Our study suggested that presence of G1896A mutation may induce severe liver injury through activation of host immune.