An exploratory study on self‐evaluated aggression and androgens in women

Healthy, premenopausal women completed the Buss‐Perry Aggression Questionnaire. Venous blood samples were taken at the mid‐cycle phase of the menstrual cycle to determine plasma androgen levels. Total and free testosterone, 5α‐dihydrotestosterone, androstenedione, and dehydroepiandrosterone were assessed. Path analyses were aimed at finding out which of the five circulating hormones might best predict a latent aggression construct derived from the four subscales of the Aggression Questionnaire. A significant correlation was found between free testosterone and physical and verbal aggression subscales. In addition, the results demonstrate for the first time in women a significant link between 5α‐dihydrotestosterone and anger. Thus, both testosterone and 5α‐dihydrotestosterone may affect, by separate pathways, the basal regulation of aggression‐proneness in women. Aggr. Behav. 28:273–280, 2002. © 2002 Wiley‐Liss, Inc.     

Adrenal mediation of intermale aggression maintained by aromatized and reduced metabolites of testosterone

Individually housed CD-1 mice were either sham castrated or castrated and treated with testosterone (T), estradiol benzoate (EB), dihydrotestosterone (DHT), a combination of EB and DHT (EB+DHT), or the injection vehicle. Following 16 days of isolation and subcutaneous injections, animals were tested repeatedly for fighting behavior in paired encounters with nonaggressive stimulus males. Results indicated that the T and EB+DHT groups fought to the same extent as the gonadally intact group. Both the EB and DHT groups fought more than the vehicle-treated group but less than the T, EB+DHT and sham castrated groups. A similar study was subsequently performed with adrenalectomized animsls. Adrenalectomy eliminated agonistic responses in animals receiving metabolites of testosterone (EB, DHT, EB+DHT) but had only slight effects in gonadally intact and T-treated, castrated mice. The results suggested that a) EB and DHT, either singly or in combination, maintain aggression through a synergism with adrenal steroids; b) the combined effects of EB and DHT reflect an additive action rather than synergistic interaction, notwithstanding the synergism with adrenal steroids; c) metabolism of testosterone to estrogen and dihydrotestosterone does not sufficiently account for the action of testosterone.