Role of Glucocorticoids on Rat Brain Metallothionein-I and -III Response to Stress

The metallothionein (MT) gene family consists of four members (MT-I through -IV) that are tightly regulated during development. Whereas MT-I and MT-II are widely expressed isoforms, MT-III has been found to be mainly expressed in the central nervous system in adult animals, and is the only isoform that inhibits survival and neurite formation of cortical neurons in vitro. A number of models of brain injury have been shown to affect MT-III mRNA levels, which has been suggested to be related to the putative neurotrophic role of this protein. However, a stress response will presumably be associated to the brain injury which could, in turn, drive MT-III regulation. In the present report the effect of a classical stress model, immobilization stress, on brain MT regulation has been studied in rats. MT-I+II protein levels were measured by radioimmunoassay in up to eight brain areas and, as expected, it was found that stress increased selectively MT-I+II levels. Adrenalectomy (ADX) had a general decreasing effect on basal MT-I+II levels; however, ADX blunted the MT-I+II response to stress in cerebellum and presumably in frontal cortex and medulla plus pons but not in the hypothalamus. MT-I mRNA measurements were in accordance with the MT-I+II protein levels in the brain areas studied. In contrast to MT-I mRNA, MT-III mRNA levels of brain cortex tended to decrease during stress, although this effect was not statistically significant. ADX also tended to decrease basal MT-III mRNA levels. Northern blot assays of pooled mRNAs suggested similar differential regulation of these two brain MT isoforms in the cerebellum. These results indicate that glucocorticoids mediate brain MT-I+II response to stress in some but not all brain areas, that a role of these hormones is likely also for MT-III, and that the regulation of MT isoforms differs substantially in the brain.     

Fitting Rasch model using appropriateness measure statistics

In this paper, the distributional properties and power rates of the Lz, Eci2z, and Eci4z statistics when they are used as item fit statistics were explored. The results were compared to t-transformation of Outfit and Infit mean square. Four sample sizes were selected: 100, 250, 500, and 1000 examinees. The abilities were uniform and normal with mean 0 and standard deviation 1, and uniform and normal with mean -1 and standard deviation 1. The pseudo-guessing parameter was fixed at .25. Two ranges of difficulty parameters were selected: +/- 1 logits and +/- 2 logits. Two test lengths were selected: 15 and 30 items. The results showed important differences between the T-infit, T-outfit, Lz, Eci2z, and Eci4z statistics. The T-oufit, T-infit, and Lz statistics showed poor standardization with estimated parameters because their distributional properties were not close to the expected values. However, the Eci2z and Eci4z statistics showed satisfactory standardization on all conditions. Further, the power rates of Eci2z and Eci4z were 5% to 10% higher than the power rates of Lz, T-outfit, and T-infit to detect items that do not fit Rasch model.     

Screening utility of the social anxiety screening scale in Spanish speaking adolescents

The aim of this study was to analyse the screening utility of the Social Anxiety Screening Scale (SASS/EDAS) in a sample of 227 adolescents with social anxiety disorder and 156 Without it (14-17 years). Results showed that the EDAS subscales (Avoidance, Distress and Interference) scores were reliable in terms of internal consistency (alpha > .80). All the subscales discriminated between adolescents with and without the disorder. They also showed a positive and significant correlation with other empirically validated measures of social anxiety. The three subscales indicated relevant sensitivity (69.16-84.14%), specificity (63.46-66.03%) and areas under the curve (.74-.81%). Binary logistic regression analyses indicated the adequate predictive utility of EDAS subscales, with the Distress subscale as the best diagnostic predictor. The data provide empirical evidence of the usefulness of EDAS as a screener for adolescent social anxiety disorder in terms of reliability, convergent and discriminant validity, diagnostic accuracy and clinical usefulness.     

Reducing uncertainty to promote appropriate decisions when facing hazardous phenomena at an active volcano

This study investigated a new direction for improving the decision making of populations at risk in the context of uncertain environmental events, such as volcanic hazards. According to the risk as feelings theory and dual process models, situations with certain outcomes do not necessarily require the use of affect heuristics (e.g., experienced feelings, anticipated emotions) as valid information in the decision‐making process. In the case of difficult decisions with moral dilemmas, certainty could even improve decisions. Thus, using an ecological design with various scenarios depicting upcoming volcanic hazards, we examined whether reducing the uncertainty of lethal threat by conveying certainty in an information campaign could influence decision making and promote a better quality of choice (correct application of the recommendations from the competent authorities). We focused specifically on two populations composed of local people living in the vicinity of an active volcano (Tungurahua volcano, Ecuador) and local scientists. We also examined whether a difficult decision involving a moral dilemma (e.g., leaving people behind so as not to jeopardize one's own life) potentiated this effect, compared to a less difficult situation without a dilemma. We demonstrated that, for local people facing a moral dilemma (difficult decision), the reduction of uncertainty of lethal threat involved a better application of the actual recommendations. These outcomes provided perspectives on improving the applications of the recommendations among local populations at risk in the context of a real risk exposure.     

Psychometric Properties and Clinical Cut-Off Scores of the Spanish Version of the Social Anxiety Scale for Adolescents

Psychotic (P) and Non-Psychotic (N-P) retardates were compared on 13 Rorschach “indicators” of psychosis using a matched pairs technique. Two of the 13 signs were found to significantly differentiate between groups with the P group demonstrating a lower F% and higher C' responding than the N-P group.     

Psychometric update of the Social Anxiety Screening Scale (SASS/EDAS) in a Spanish adolescent population

The aim of this work was to update the validation of the Social Anxiety Screening Scale (SASS/EDAS) in a sample of Spanish adolescents. To achieve this, one study with a sample of 1489 students in secondary education, of ages 14 to 17 years, were carried out. The psychometric properties of EDAS were examined through confirmatory factor analysis, reliability (Cronbach's alpha), temporal stability (test-retest), and concurrent criterion validity. The results supported the three-independent-factor structure (avoidance, distress and interference), that showed best fit indices compared to alternative models. They also showed that the scores of participants on EDAS scales were reliable in terms of internal consistency (alpha > .80) and moderately reliable concerning temporal stability (r = .48-.60) over a five-week period. The correlations between the EDAS factors and other social anxiety measures were positive and significant. Data provide empirical evidence of the estimation of reliability and validity of this scale. Future work should extend the validation of the EDAS in clinical samples.     

The Social Phobia Inventory: screening and cross-cultural validation in Spanish adolescents

Availability of brief, self-report measures to be used as screening instruments is crucial to detect correctly youth with social anxiety disorder and therefore, reach those otherwise under-detected and under-treated. A previous study revealed that the Social Phobia Inventory (SPIN) was potentially an appropriate measure for screening social anxiety among US adolescents. However, there is a lack of information concerning its properties as a screening test in other cultures and languages. This is the main objective of this study, although further validity of the scale is provided as well. The sample consisted of 192 adolescents (a sample composed of 114 subjects with a principal diagnosis of social anxiety disorder; and a group consisting of 78 subjects with no diagnosis of social phobia). Results suggest that the Social Phobia Inventory has demonstrated good psychometric properties and indeed may be used as a screening tool in Spanish-speaking adolescents.     

Effect of drugs on the cardiac conditioned response of dogs

Dogs were conditioned to an anxiety response characterized by an increase in heart rate upon presentation of a musical tone. Meprobamate, chlordiazepoxide and diazepam effectively prevented the cardiac conditioned response. Phenobarbital and chlorpromazine were less effective. Amphetamine, 1.5 orally, also blocked the cardiac conditioned response. Atropine, meperidine, caffeine, imipramine and -methyl-dopa had no effect on the cardiac conditioned response. Morphine and methyl phenidate were clearly active only in doses producing marked side effects. Ethanol in relatively small doses was mildly effective.     

Are implicitly activated associates selectively activated?

What constitutes a word’s associative past? Words differ in how many associates they activate in memory and, following a brief encounter, those with fewer associates are more likely to be recalled in the presence of related cues. The issue addressed in the present article is whether associative set size effects are produced through the selective activation of strong associates or through the activation of both strong and weak associates. The set size of the strongest associates was varied factorially with the set size of the associates of these associates. We assume that associate set size indexes a word’s weaker associates. Experiments 1 and 2 showed that recall varied inversely with both target and associate set sizes. Such results held over variations in study time and participant age. Experiment 3 showed that weak associates of the target had a greater effect on recall when there were more connections among the strongest associates in the set. The findings suggest that activation is not strength selective but includes both weak and strong associates.     

The Social Phobia and Anxiety Inventory: Reliability and Validity in an Adolescent Spanish Population

The objective of this study is to examine the factor structure and psychometric properties of the SPAI in a sample of adolescent Spaniards. The results obtained support the use of the correlated two-factor SPAI subscales and indicate high coefficient alpha values for the SPAI subscales. The results provide support for the use of the SPAI in an adolescent population in a non-English-speaking country. Both exploratory and confirmatory factor analyses of the Social Phobia subscale showed a four-factor structure, instead of a five-factor dimension, as suggested by previous studies. Effects for gender and age and gender interaction were found. Limitations and suggestions for future research are discussed.