Increased Error-Related Brain Activity in Six-Year-Old Children with Clinical Anxiety

Anxiety disorders are the most frequently diagnosed form of psychopathology in children and often result in chronic impairment that persists into adulthood. Identifying neurobehavioral correlates of anxiety that appear relatively early in life would inform etiological models of development and allow intervention and prevention strategies to be implemented more effectively. The error-related negativity (ERN), a negative deflection in the event-related potential at fronto-central sites approximately 50 ms following the commission of errors, has been consistently found to be larger among anxious adults. The current study sought to extend these findings to even younger individuals: the ERN was elicited by a Go/NoGo task in 48 six year-old children with a clinical anxiety disorder assessed by diagnostic interview and 48 age-matched controls. In addition to child anxiety disorder, the ERN was examined in relation to maternal history of anxiety disorder, which was previously related to a smaller ERN. Anxious children were characterized by a larger (i.e., more negative) ERN and maternal history of anxiety disorder was associated with a smaller ERN. Thus, the relationship between an increased ERN and clinical anxiety is evident by age 6, and this effect appears independent from an opposing influence of maternal anxiety history on the ERN. These findings support the ERN as a promising neurobehavioral marker of anxiety, and implications are discussed.     

The effect of trial-to-trial feedback on the error-related negativity and its relationship with anxiety

Individuals with anxiety disorders and related personality traits are characterized by increased error-related brain activity, as measured by the error-related negativity (ERN) in simple speeded response tasks. An absent, or opposite, relation between anxiety and the ERN has been reported in studies that employed reinforcement learning paradigms with trial-to-trial feedback. Understanding the effect of trial-to-trial feedback on the ERN may help clarify these results and can further elucidate the impact of feedback on performance monitoring. In the present study, 30 undergraduate participants performed two versions of the arrowhead version of the flanker task in counterbalanced order: one with trial-to-trial feedback and one without. The participants were slower and more accurate in the task with trial-to-trial feedback; however, the ERN was equivalent between the two tasks. Larger ERNs were related to higher trait anxiety, but only in the version without trial-to-trial feedback. These findings show that although trial-to-trial performance feedback impacts behavioral measures, it does not affect the ERN; moreover, the presence of trial-to-trial feedback moderates the relationship between the ERN and anxiety.     

Working memory load reduces the late positive potential and this effect is attenuated with increasing anxiety

Emotion regulation decreases the processing of arousing stimuli, as indexed by the late positive potential (LPP), an electrocortical component that varies in amplitude with emotional arousal. Emotion regulation increases activity in the prefrontal areas associated with cognitive control, including the dosolateral prefrontal cortex (DLPFC). The present study manipulated working memory load, known to activate the DLPFC, and recorded the LPP elicited by aversive and neutral IAPS pictures presented during the retention interval. The LPP was larger on low-load compared to high-load trials, and on trials with aversive compared to neutral pictures. These LPP data suggest that emotional content and working memory load have opposing effects on attention to distracting stimuli. State anxiety was associated with reduced modulation of the LPP by working memory load. Results are discussed in terms of competition for attention between emotion and cognition and suggest a relationship between DLPFC activation and the allocation of attentional resources to distracting visual stimuli–a relationship that may be disrupted with increasing anxiety.     

Psychometric properties of the OCI-R in a college sample

Two studies examined the psychometric properties of the Obsessive-Compulsive Inventory-Revised (OCI-R; Psychol. Assessment 14 (2002) 485) in a nonclinical student sample. In Study 1, we investigated the factor structure and internal consistency of the OCI-R using a sample of 395 undergraduate students. At a second testing session 1 month later, 178 students completed the OCI-R. Test-retest reliability was examined using data from 94 students who completed the OCI-R in both sessions. Convergent validity was also assessed with the Maudsley Obsessive-Compulsive Inventory (MOCI). In Study 2, we further investigated the convergent and divergent validity of the OCI-R using a new sample of 221 students who completed a battery of measures of obsessive-compulsive symptoms, worry, and depression. There was a significant order effect for both the OCI-R and the MOCI: means of each measure were significantly lower when presented second. Despite the order effect, statistical analyses indicated that the OCI-R has adequate test-retest reliability for the full scale and subscale scores, solid factor structure, and high internal consistency. Convergent validity with other measures of obsessive-compulsive symptoms was moderate to excellent, and divergent validity was good. The results indicate that the OCI-R is a short, psychometrically sound self-report measure of obsessive-compulsive symptoms.     

Emotion regulation and amygdala-precuneus connectivity: Focusing on attentional deployment

Attentional deployment is an emotion regulation strategy that involves shifting attentional focus. Deploying attention to non-arousing, compared to arousing, regions of unpleasant images has been associated with reduced negative affect, reduced amygdala activation, and increased activity in fronto-parietal control networks. The current study examined neural correlates and functional connectivity associated with using attentional deployment to increase negative affect (deploying attention towards arousing unpleasant information) or to decrease negative affect (deploying attention away from arousing unpleasant information), compared to naturally viewing unpleasant images, in 42 individuals while concurrently monitoring eye movements. Directing attention to both arousing and non-arousing regions resulted in enhanced fronto-parietal activation compared to natural viewing, but only directing attention to non-arousing regions was associated with changes in amygdala activation. There were no significant differences in connectivity between naturally viewing unpleasant images and focusing on arousing regions. However, naturally viewing unpleasant images, relative to focusing on non-arousing regions, was associated with increased connectivity between the amygdala and visual cortex, while focusing on non-arousing regions of unpleasant images, compared to natural viewing, was associated with increased connectivity between the amygdala and the precuneus. Amygdala-precuneus connectivity correlated positively with eye-tracking measures of attentional deployment success and with trait reappraisal. Deploying attention away from arousing unpleasant information, then, may depend upon functional relationships between the amygdala and parietal regions implicated in attentional control. Furthermore, these relationships might relate to the ability to successfully implement attentional deployment, and the predisposition to utilize adaptive emotion regulation strategies.     

The orienting of spatial attention to backward masked fearful faces is associated with variation in the serotonin transporter gene

Threat signals facilitate spatial attention, even when awareness of these signals has been restricted through the use of backward masking. However, unrestricted/unmasked threat cues tend to delay the disengagement of attention, whereas restricted/masked threat facilitates orienting, suggesting different underlying mechanisms. Within the general population, the serotonin transporter gene polymorphism (5HTTLPR) is associated with one's allocation of attention to unmasked threat signals. However, it is unclear to what extent the 5HTTLPR gene may be involved in nonconscious biases to masked threat, and whether or not such biases are driven by facilitated orienting or delayed disengagement. Participants were genotyped and performed a dot-probe task with backward masked fearful and neutral faces. Results indicate that short-allele carriers of the 5HTTLPR gene nonconsciously orient spatial attention to masked fearful faces. On the other hand, homozygous long-allele individuals tended to direct attention away from masked fearful faces. All participants' performance was at chance in a posttask assessment of awareness for the masked faces. The results add to current literature on the 5HTTLPR and attention biases, and suggest that threat signals facilitate the orienting of attention in short-allele carriers of the 5HTTLPR gene even under restricted processing conditions.     

Better late than never? The effect of feedback delay on ERP indices of reward processing

The feedback negativity (FN), an early neural response that differentiates rewards from losses, appears to be generated in part by reward circuits in the brain. A prominent model of the FN suggests that it reflects learning processes by which environmental feedback shapes behavior. Although there is evidence that human behavior is more strongly influenced by rewards that quickly follow actions, in nonlaboratory settings, optimal behaviors are not always followed by immediate rewards. However, it is not clear how the introduction of a delay between response selection and feedback impacts the FN. Thus, the present study used a simple forced choice gambling task to elicit the FN, in which feedback about rewards and losses was presented after either 1 or 6?s. Results suggest that, at short delays (1?s), participants clearly differentiated losses from rewards, as evidenced in the magnitude of the FN. At long delays (6?s), on the other hand, the difference between losses and rewards was negligible. Results are discussed in terms of eligibility traces and the reinforcement learning model of the FN.     

Event-related induced frontal alpha as a marker of lateral prefrontal cortex activation during cognitive reappraisal

Electrocortical activity, typically used to track the effects of cognitive reappraisal on the processing of emotional stimuli, has not been used to index the prefrontal-cortex-mediated regulatory mechanisms responsible for these effects. In the present study, we examined the novel possibility that induced frontal alpha (i.e., 8?C13?Hz), shown to reflect the inhibition and disengagement of task-relevant cortical regions, may be quantified to explore cortical activation that is specifically enhanced during cognitive reappraisal. For this purpose, 44 participants viewed unpleasant and neutral pictures followed by auditory instructions to either continue viewing the picture or reduce their emotional response to the picture by making the picture seem less emotional (i.e., cognitive reappraisal). In line with previous work, unpleasant pictures elicited a larger late positive potential (LPP) than did neutral pictures. Also corroborating previous work, the mid-latency LPP was reduced when pictures were cognitively reappraised. However, the present study showed for the first time that whereas unpleasant pictures elicited higher frontal alpha power bilaterally than did the neutral pictures, frontal alpha power was reduced (indicative of more activation and cognitive control) during cognitive reappraisal of both picture types over the left hemisphere. Taken together, the LPP and event-related induced frontal-alpha findings contribute unique information about the distinct neural substrates and cognitive processes underlying reappraisal.     

Attentional Biases for Emotional Faces in Young Children of Mothers with Chronic or Recurrent Depression

Attentional biases for negative stimuli have been observed in school-age and adolescent children of depressed mothers and may reflect a vulnerability to depression. The direction of these biases and whether they can be identified in early childhood remains unclear. The current study examined attentional biases in 5–7-year-old children of depressed and non-depressed mothers. Following a mood induction, children participated in a dot-probe task assessing biases for sad and happy faces. There was a significant interaction of group and sex: daughters of depressed mothers attended selectively to sad faces, while children of controls and sons of depressed mothers did not exhibit biases. No effects were found for happy stimuli. These findings suggest that attentional biases are discernible in early childhood and may be vulnerability markers for depression. The results also raise the possibility that sex differences in cognitive biases are evident before the emergence of sex differences in the prevalence of depression.