Role of Glucocorticoids on Rat Brain Metallothionein-I and -III Response to Stress

The metallothionein (MT) gene family consists of four members (MT-I through -IV) that are tightly regulated during development. Whereas MT-I and MT-II are widely expressed isoforms, MT-III has been found to be mainly expressed in the central nervous system in adult animals, and is the only isoform that inhibits survival and neurite formation of cortical neurons in vitro. A number of models of brain injury have been shown to affect MT-III mRNA levels, which has been suggested to be related to the putative neurotrophic role of this protein. However, a stress response will presumably be associated to the brain injury which could, in turn, drive MT-III regulation. In the present report the effect of a classical stress model, immobilization stress, on brain MT regulation has been studied in rats. MT-I+II protein levels were measured by radioimmunoassay in up to eight brain areas and, as expected, it was found that stress increased selectively MT-I+II levels. Adrenalectomy (ADX) had a general decreasing effect on basal MT-I+II levels; however, ADX blunted the MT-I+II response to stress in cerebellum and presumably in frontal cortex and medulla plus pons but not in the hypothalamus. MT-I mRNA measurements were in accordance with the MT-I+II protein levels in the brain areas studied. In contrast to MT-I mRNA, MT-III mRNA levels of brain cortex tended to decrease during stress, although this effect was not statistically significant. ADX also tended to decrease basal MT-III mRNA levels. Northern blot assays of pooled mRNAs suggested similar differential regulation of these two brain MT isoforms in the cerebellum. These results indicate that glucocorticoids mediate brain MT-I+II response to stress in some but not all brain areas, that a role of these hormones is likely also for MT-III, and that the regulation of MT isoforms differs substantially in the brain.     

Confirmatory factor analysis of parents' and teachers' ratings of DSM-IV symptoms of attention deficit hyperactivity disorder in a Spanish sample

Confirmatory factor analysis examined the factor structure of DSM IV Attention Deficit Hyperactivity Disorder symptoms and analyzed the differences in informants' ratings of ADHD symptoms by children's age and sex. A sample of 1,018 Spanish school children, 574 girls and 444 boys, ages 4 to 12 years, was rated by teachers and parents. Confirmatory factor analysis showed a similar fit for the three-factor model of Inattention, Hyperactivity, and Impulsivity (teachers: CFI = .976, RMR = .041, % of total variance = 83.2; parents: CFI = .969, RMR = .037, % of total variance = 82.7) and for the two-factor model of Inattention and Hyperactivity Impulsivity (teachers: CFI = .958, RMR = .044, % of total variance = 79.3; parents: CFI = .961, RMR = .038, % of total variance, 79.9). The two-factor model was selected as a parsi monious representation of the teachers' and parents' ratings of ADHD symptoms. Analyses of variance indicated significant differences in teachers' and parents' mean ratings across sex and school grades of children.     

Metarepresentative functions in borderline personality disorder

Many authors consider that patients suffering from Borderline Personality Disorder (BPD) are hampered in their ability to metarepresent, which is the correct ascribing of states of mind to oneself and to others and the reflecting thereon. Although the ability to mentalize is generally described as being uniform, various authors pinpoint problems which appear to be of a diverse psychological nature. Some describe difficulties in identifying emotions or a shortfall in their regulation, others identify a lack of integration between representations of self and those of others, and yet others focus on the failure to distinguish between fantasy and reality. In the present research all sessions during the first year of therapy of four patients suffering from BPD were tape-recorded and transcribed, and then analyzed using the Metacognition Assessment Scale (MAS), which is designed for the evaluation of the ability to metarepresent in clinical reports. The results support the hypothesis that there is a metarepresentation impairment in BPD but that it is more selective than was thought until now. In particular, such patients maintain their ability to identify internal states, whereas they are impaired in the integration of representations of self and others and in the differentiation between fantasy and reality.     

Fitting Rasch model using appropriateness measure statistics

In this paper, the distributional properties and power rates of the Lz, Eci2z, and Eci4z statistics when they are used as item fit statistics were explored. The results were compared to t-transformation of Outfit and Infit mean square. Four sample sizes were selected: 100, 250, 500, and 1000 examinees. The abilities were uniform and normal with mean 0 and standard deviation 1, and uniform and normal with mean -1 and standard deviation 1. The pseudo-guessing parameter was fixed at .25. Two ranges of difficulty parameters were selected: +/- 1 logits and +/- 2 logits. Two test lengths were selected: 15 and 30 items. The results showed important differences between the T-infit, T-outfit, Lz, Eci2z, and Eci4z statistics. The T-oufit, T-infit, and Lz statistics showed poor standardization with estimated parameters because their distributional properties were not close to the expected values. However, the Eci2z and Eci4z statistics showed satisfactory standardization on all conditions. Further, the power rates of Eci2z and Eci4z were 5% to 10% higher than the power rates of Lz, T-outfit, and T-infit to detect items that do not fit Rasch model.     

The force of symmetry revisited: symmetry-to-noise ratios regulate (a)symmetry effects

Freyd and Tversky's [Am. J. Psychol. 97 (1984) 109] data suggested that human observers tend to overestimate relatively high levels of symmetry (symmetry effect), and tend to underestimate relatively low levels of symmetry (asymmetry effect). However, on the basis of their holographic approach to visual regularity, van der Helm and Leeuwenberg [Psychol. Rev. 103(3) (1996) 429] predicted that, at any level of symmetry, both symmetry and asymmetry effects may occur as a consequence of correct estimates of symmetry-to-noise ratios. This prediction was tested in two experiments, with tasks and stimuli similar to those in Freyd and Tversky's study. First, subjects had to judge whether a noisy symmetry is more similar to a slightly more symmetrical variant or to a slightly less symmetrical variant. Second, for every pair of stimuli in such a triadic comparison, subjects had to judge which stimulus is the more symmetrical one. The results from both experiments show that the occurrence of (a)symmetry effects indeed depends on symmetry-to-noise ratios.     

Subgroup analysis of sex difference on the Vandenberg-Kuse mental rotation test

A large sex difference has been elicited on the Vandenberg-Kuse mental rotation test. Prior research emphasizes the biological root of this sex difference. In recent experiments we confirmed this viewpoint. A large sample was administered the test, and the distributions of scores for men and women (N = 138; 68 men and 70 women: ages 19 to 23 years). The mean scores were used as cut-off points to group the men and the women in different subgroups (Low/Women, High/Women, Low/Men, High/Men). There were large differences among all subgroups, reinforcing Kimura's testosterone hypothesis for sex differences in spatial ability.     

Screening utility of the social anxiety screening scale in Spanish speaking adolescents

The aim of this study was to analyse the screening utility of the Social Anxiety Screening Scale (SASS/EDAS) in a sample of 227 adolescents with social anxiety disorder and 156 Without it (14-17 years). Results showed that the EDAS subscales (Avoidance, Distress and Interference) scores were reliable in terms of internal consistency (alpha > .80). All the subscales discriminated between adolescents with and without the disorder. They also showed a positive and significant correlation with other empirically validated measures of social anxiety. The three subscales indicated relevant sensitivity (69.16-84.14%), specificity (63.46-66.03%) and areas under the curve (.74-.81%). Binary logistic regression analyses indicated the adequate predictive utility of EDAS subscales, with the Distress subscale as the best diagnostic predictor. The data provide empirical evidence of the usefulness of EDAS as a screener for adolescent social anxiety disorder in terms of reliability, convergent and discriminant validity, diagnostic accuracy and clinical usefulness.     

Effect of an aerobic training program as complementary therapy in patients with moderate depression

The aim of this study was to assess the effects of an aerobic training program as complementary therapy in patients suffering from moderate depression. 82 female patients weredivided into a group that received traditional pharmacotherapy (Fluoxetine 20 mg) and a group that received pharmacotherapy plus an aerobic training program. This program was carried out for eight consecutive weeks, three days per week, and included gymnastics, dancing, and walking. Depressive symptoms were measured with the Beck Depression Inventory and the ICD-10 Guide for Depression Diagnosis, both administered before and after treatments. The results confirm the effectiveness of the aerobic training program as a complementary therapy to diminish depressive symptoms in patients suffering from moderate depression.     

Reducing uncertainty to promote appropriate decisions when facing hazardous phenomena at an active volcano

This study investigated a new direction for improving the decision making of populations at risk in the context of uncertain environmental events, such as volcanic hazards. According to the risk as feelings theory and dual process models, situations with certain outcomes do not necessarily require the use of affect heuristics (e.g., experienced feelings, anticipated emotions) as valid information in the decision‐making process. In the case of difficult decisions with moral dilemmas, certainty could even improve decisions. Thus, using an ecological design with various scenarios depicting upcoming volcanic hazards, we examined whether reducing the uncertainty of lethal threat by conveying certainty in an information campaign could influence decision making and promote a better quality of choice (correct application of the recommendations from the competent authorities). We focused specifically on two populations composed of local people living in the vicinity of an active volcano (Tungurahua volcano, Ecuador) and local scientists. We also examined whether a difficult decision involving a moral dilemma (e.g., leaving people behind so as not to jeopardize one's own life) potentiated this effect, compared to a less difficult situation without a dilemma. We demonstrated that, for local people facing a moral dilemma (difficult decision), the reduction of uncertainty of lethal threat involved a better application of the actual recommendations. These outcomes provided perspectives on improving the applications of the recommendations among local populations at risk in the context of a real risk exposure.     

My Life as a Psychoanalyst in Spain

This article attempts to explain, in a necessarily brief way, the reasons underlying my choice of the psychiatry specialty: why I chose the psychoanalytically oriented psychotherapy, the authors and theories that have influenced me the most, and my current professional situation, making some allusion to my hobbies.