In Vivo Hippocampal Acetylcholine Release During Exposure to Acute Stress

Hippocampal extracellular acetylcholine (ACh) and choline levels were evaluated using in vivo microdialysis in male Fischer 344 rats before, during, and following an 80-min exposure to two different stress conditions. Measurements were taken in rats restrained and immersed in a water bath containing either 37 degreesC (normothermic-restraint) or 20 degreesC (cold-restraint) water. Results were compared to normothermic-freely-moving rats. Cold-restrained rats displayed decreased ACh levels during cold exposure relative to both normothermic-restrained and normothermic-freely-moving rats. By the end of the cold exposure period and following removal from cold, ACh levels had returned to near-baseline values. Normothermic-restrained rats had levels similar to those of normothermic-freely-moving rats, except for a marked increase in ACh following removal from restraint. Cold-restrained rats displayed a gradual elevation in choline levels during cold stress, followed by a gradual decline after stress termination, whereas both normothermic-restrained and normothermic-freely-moving rats displayed gradual decreases during the microdialysis session. These findings demonstrate that central cholinergic neurotransmission can be altered by the application of, and removal from, acute stressors. In addition, the results suggest a possible relationship between the magnitudes of both the stressor and its cholinergic consequences.     

Restlessness and psychomotor agitation after kidney transplantation: their impact on perceived health status

Restlessness and psychomotor agitation are among the causes of morbidity and mortality in different diseases. They are known problems in end-stage renal disease patients but no study has ever evaluated their presence and effects in kidney recipients. This study aimed to explore the presence of restlessness and psychomotor agitation and their relation with morbidity of patients after renal transplantation. Sixty-one subjects were randomly selected from kidney transplant recipients being followed in Baqiyatallah Hospital, Tehran, Iran. Restlessness and psychomotor agitation were determined by Hospital Anxiety and Depression Scale (HADS). The correlation of these scores with demographic data, clinical data, sexual function, marital adjustment, quality of life, sleep quality and medical comorbidity of patients was evaluated. The scores of restlessness and psychomotor agitation were significantly correlated with the age at transplantation (r = .269, p = .04 and r = .324, p = .01, respectively), the total score of medical comorbidity (r = .273, p = .02 and r = .257, p = .04, respectively), role limitation due to emotional problems (r = -.26, p = .03 and r = -.25, p = .04, respectively) and mental health (r = -.277, p = .02 and r = -.252, p = .04 respectively). There was no correlation between these symptoms and the gender, marital status, and sexual relationship score of the patients (p > .05). Restlessness and psychomotor agitation are frequent and can impose high degrees of morbidity on renal transplant recipients. Proper management of these problems seems to improve the different aspects of quality of life in these patients.