MAP2 and synaptophysin protein expression following motor learning suggests dynamic regulation and distinct alterations coinciding with synaptogenesis

Learning a new motor skill can induce neuronal plasticity in rats. Within motor cortex, learning-induced plasticity includes dendritic reorganization, synaptogenesis, and changes in synapse morphology. Behavioral studies have demonstrated that learning requires protein synthesis. It is likely that some of the proteins synthesized during learning are involved in, or the result of, learning-induced structural plasticity. We predicted the expression of proteins involved in neural plasticity would be altered in a learning dependent fashion. Long-Evans rats were trained on a series of motor tasks that varied in complexity, so that the effects of activity could be teased apart from the effects of learning. The motor cortices were examined for MAP2 and synaptophysin protein using Western blotting and immunohistochemistry. Western blotting revealed that expression of MAP2 was not detectably influenced by learning, whereas synaptophysin expression increased on day 1, 3, and 5 of complex motor skill learning. Expression of MAP2 does not seem to indicate difficulty of task or duration of training time, whereas increases in synaptophysin expression, which appear diffusely across the cortex, seem to be correlated with the first 5 days of motor skill learning. Similar findings with GAP-43 suggest the change in synaptophysin may coincide with synapse formation. Immunohistochemistry did not reveal any localized changes in protein expression. These data indicate a difference in learning-induced expression in the mammalian brain compared to reports in the literature, which have often focused on stimulation to induce alterations in protein expression.     

Facilitation of recall with intralist cues: Time-dependent characteristics of cued recall

List items were given as retrieval cues in a free-recall experiment which factorially combined the presence or absence of cues with the amount of time allowed for use of each cue (10 sec or 30 sec). A categorizable list of 75 randomly presented words was learned, and 48 h later a free-recall test trial was given, followed by a final memory search task. During the final task, cued subjects received words from categories that had not been recalled during the free-recall test. With both time intervals, cued subjects recalled more words than noncued subjects, indicating that random presentation of categorized words does not necessarily preclude the observation of a cueing effect with list items, as has been reported previously. The composition of recall, whether from previously recalled or nonrecalled categories, varied as a function of time for both groups. The results were interpreteod in terms of retrieval strategies employed by cued and noncued subjects and the effect of time on these strategies.