鉴定干细胞源胰岛素分泌细胞的方法及其利弊

在诱导各种干细胞向胰岛素分泌细胞(insulin producing cells,IPCs)分化的研究中,或多或少地存在着分化效率低、分化细胞不够成熟以及分泌产物的性质难以确定等问题,而IPCs的功能决定了其治疗糖尿病的效果,也是衡量各种诱导方案的重要指标。如何合理、科学地对IPCs进行形态与功能鉴定本文对此加以探讨。     

Stressful life events and suicidal behaviour in countries with different development levels: Nicaragua,El Salvador,Chile and Spain

This paper presents a study conducted on 709 Latin American undergraduates from four countries with different development levels (Nicaragua, El Salvador, Chile and Spain). The intention of this paper is to value the differences in the number and characteristics of stressful life events (SLE) suffered by the participants. Another purpose is to confirm the relation between SLE and suicidal conduct in Spanish cultural contexts, through the study of a multicultural sample of Spanish‐speaking university students, and finally, to isolate the SLE that allow predicting a later suicidal conduct to a greatest extent. The information was collected by means of a self‐applied questionnaire. The results indicate a greater presence of SLE among those who live in countries with lower development levels and among those who have attempted committing suicide. It is observed that the fact of having suffered certain SLE—physical or sexual mistreatment, excessive alcohol or drug consumption and having left home during childhood or adolescence—aid in the prediction of later suicidal conducts in the studied samples. Copyright © 2010 John Wiley & Sons, Ltd.     

Group-based problem-solving therapy in self-poisoning females: A pilot study

Aims: The treatment of suicidal behaviour remains limited in efficacy. This pilot study assessed the effectiveness of a time-limited, group-based problem-solving therapy intervention compared with a treatment as usual control group, in females who self-poison. Method: A total of 18 clients were equally randomised to treatment or control groups. All clients were assessed using standardised questionnaires for depression, hopelessness, suicidal ideation and social problem-solving skills, at pre-treatment, post-treatment and two months follow-up. Results: The treatment group experienced significant reductions in levels of depression, hopelessness, suicidal ideation and improvements in self-assessed social problem-solving skills. Improvements in mental health and aspects of self-assessed problem-solving skills for the treatment group continued to be evident at two months follow-up. The control group did not change significantly over time on mental health measures or social problem solving abilities. Conclusion: Although limited by small sample size, the results suggest that group-based problem-solving therapy is effective in the management of deliberate self-poisoning. This paper is a unique contribution in that it examines the implementation of problem solving therapy with a homogenous population and in a group format. Methodological concerns and directions for future research are discussed.     

Young children’s acquisition of knowledge about the Earth: A longitudinal study

This longitudinal study examined the acquisition of early knowledge of astronomy to determine whether children’s knowledge at any point in time is consistent with a naive “mental model.” Children were first assessed by means of open questions and drawing tasks at 2 and 3 years of age (N = 143). The knowledge was reassessed over the course of the following 3 years. The results showed that although a few indications of naive mental models were found, in most cases young children’s knowledge was fragmented and accurate knowledge was often expressed alongside inaccurate/synthetic ideas. Furthermore, it was shown that children need to know scientific facts before they start taking the global perspective when describing the world and, when faced with ambiguous open questions, children often experience difficulties that can induce them to change the types of answers they provide.     

Prior sensitivity in theory testing: An apologia for the Bayes factor

A commonly voiced concern with the Bayes factor is that, unlike many other Bayesian and non-Bayesian quantitative measures of model evaluation, it is highly sensitive to the parameter prior. This paper argues that, when dealing with psychological models that are quantitatively instantiated theories, being sensitive to the prior is an attractive feature of a model evaluation measure. This assertion follows from the observation that in psychological models parameters are not completely unknown, but correspond to psychological variables about which theory often exists. This theory can be formally captured in the prior range and prior distribution of the parameters, indicating which parameter values are allowed, likely, unlikely and forbidden. Because the prior is a vehicle for expressing psychological theory, it should, like the model equation, be considered as an integral part of the model. It is argued that the combined practice of building models using informative priors, and evaluating models using prior sensitive measures advances knowledge.     

Asian American Female College Students' Subjective Femininity Conceptualizations: Using a Mixed‐Methods Approach

This mixed‐methods study explored 184 Asian American female college students' subjective femininity conceptualizations and their associations with family conflict. A direct content analysis of responses identified 5 categories of subjective femininity conceptualizations: model minority, physical attributes, traditional femininity, subordinate roles, and modern femininity. Conceptualizations of subordinate roles were positively related to intensity of family conflict. Interdependent self‐construal moderated the relationship between subjective conceptualizations of subordinate roles, modern femininity, and intensity of family conflict. Clinical implications of these findings are discussed. Este estudio de métodos combinados exploró las conceptualizaciones subjetivas de feminidad y sus asociaciones con conflictos familiares entre 184 estudiantes universitarias asiático‐americanas. Un análisis directo del contenido de las respuestas identificó 5 categorías de conceptualizaciones subjetivas de feminidad: modelo de minoría, atributos físicos, feminidad tradicional, roles subordinados y feminidad moderna. Las conceptualizaciones de roles subordinados se relacionaron de forma positiva con la intensidad de los conflictos familiares. La autoconstrucción interdependiente moderó la relación entre las conceptualizaciones subjetivas de roles subordinados, la feminidad moderna y la intensidad de los conflictos familiares. Se discuten las implicaciones clínicas de estos hallazgos.     

论死亡的尊严与权利——以癌症病人自杀现象为例

现代医学对晚期癌症病人死亡过程的过分干预使得死亡的尊严与权利问题日益受到人们的重视。而对病人自杀现象的理解则更应该借助于尊严死亡的理念。通过文献研究认为,难以忍受的躯体和心理上的痛苦是造成癌症病人自杀的首要原因。因此,癌症病人的自杀行为可以在相当程度上解释为一种摆脱艰难生存境遇的尝试。医生和家属都应充分考量癌症病人生命最后阶段的生活质量,给予他们真挚的关爱,在必要的时候,应允许癌症病人选择一种坦然的、平静的、有尊严的死亡方式,赋予他们选择死亡的权利。     

质性研究中的资料饱和及其判定

在质性研究中,资料饱和通常被用以评估研究资料的充足性。但在研究实践中,资料饱和却存在概念模糊及操作性问题。作为某项质性研究所抽取的样本量已满足研究需要的标志,按照在研究过程中判定时点的先后顺序,资料饱和可划分为数据饱和、编码或主题饱和、意义饱和及理论饱和4种主要形式。4种形式的资料饱和各有其特定的内涵、评估方式和判定标准。研究认为,达到资料饱和的样本量标准不应统一设定,其检验需嵌入具体的研究过程;资料饱和存在逻辑上的不确定性,适度追加抽样有助于进一步确认;资料饱和作为考察研究质量的一项重要指标,并不适用于所有质性研究。     

Assessing Biobehavioural Self‐Regulation and Coregulation in Early Childhood: The Parent‐Child Challenge Task

Researchers have argued for more dynamic and contextually relevant measures of regulatory processes in interpersonal interactions. In response, we introduce and examine the effectiveness of a new task, the Parent–Child Challenge Task, designed to assess the self‐regulation and coregulation of affect, goal‐directed behaviour, and physiology in parents and their preschoolers in response to an experimental perturbation. Concurrent and predictive validity was examined via relations with children's externalizing behaviours. Mothers used only their words to guide their 3‐year‐old children to complete increasingly difficult puzzles in order to win a prize (N = 96). A challenge condition was initiated midway through the task with a newly introduced time limit. The challenge produced decreases in parental teaching and dyadic behavioural variability and increases in child negative affect and dyadic affective variability, measured by dynamic systems‐based methods. Children rated lower on externalizing showed respiratory sinus arrhythmia (RSA) suppression in response to challenge, whereas those rated higher on externalizing showed RSA augmentation. Additionally, select task changes in affect, behaviour, and physiology predicted teacher‐rated externalizing behaviours four months later. Findings indicate that the Parent–Child Challenge Task was effective in producing regulatory changes and suggest its utility in assessing biobehavioural self‐regulation and coregulation in parents and their preschoolers. Copyright © 2016 John Wiley & Sons, Ltd.