慢性应激对大鼠行为和免疫细胞热休克蛋白70表达的影响

目的：研究慢性不确定应激对大鼠急性整体热水浴后外周血和脾脏免疫细胞热休克蛋白70（Heat shock protein 70, HSP70）表达的影响。方法：随机将大鼠分成慢性应激组和控制组（每组14只）。通过4周的慢性不确定性应激诱发实验组大鼠明显的抑郁行为,此期间控制组大鼠正常饲养。随后给予大鼠42度整体热水浴刺激,维持直肠温度41度25min。热刺激后6h,采用流式细胞仪测定大鼠外周血和脾脏免疫细胞HSP70 水平。结果：与控制组大鼠相比,慢性应激大鼠在急性热刺激后HSP70合成明显减少。控制组大鼠的所有被检测的免疫细胞热应激后HSP70合成均明显增加。相反,慢性应激大鼠仅在外周血的单核细胞和粒细胞检测到HSP70合成增加,同时升高的水平明显低于控制组大鼠。结论：慢性应激降低大鼠免疫细胞HSP70的热诱导反应,提示HSP70保护性作用减弱可能参与了慢性应激损害免疫细胞功能的生物学过程     

Coping with anxiety and rebuilding identity: A psychosynthesis approach to culture shock

As the number of international students continues to increase worldwide, their experience of culture shock has been well-researched. Missing, however, from the culture shock literature is the perspective of psychosynthesis psychology and its methodology to deal with the affective, behavioral, and cognitive (ABC) aspects of shock and adaptation. This article illustrates two psychosynthesis techniques that student sojourners found helpful in their acculturation process. Firstly, the self-identification exercise eased anxiety, an affective aspect of culture shock. Secondly, the subpersonality model aided students in their ability to integrate a new social identity, the cornerstone of the cognitive aspect. With a new integrated identity, students changed their behavior (the second ABC component) and new creative energies were released. These qualitative findings are supported by the testimonies of nine international student sojourners in the Netherlands who received psychosynthesis counseling. In addition, a case study demonstrates subpersonality integration and its role in helping students to come into relationship with themselves as well as others. The self-identification exercise and subpersonality model are not only effective tools for aiding sojourners in their adaptation to a host country, but viable methodologies for anyone searching to synthesize a new personal and social identity.     

Grain orientation effects on dynamic strength of FCC multicrystals at low shock pressures: a hydrodynamic instability study

Variability in local dynamic plasticity due to material anisotropy in polycrystalline metals is likely to be important on damage nucleation and growth at low pressures. Hydrodynamic instabilities could be used to study these plasticity effects by correlating measured changes in perturbation amplitudes at free surfaces to local plastic behaviour and grain orientation, but amplitude changes are typically too small to be measured reliably at low pressures using conventional diagnostics. Correlations between strength at low shock pressures and grain orientation were studied in copper (grain size?≈?800 μm) using the Richtmyer–Meshkov instability with a square-wave surface perturbation (wavelength = 150 μm, amplitude = 5 μm), shocked at 2.7 GPa using symmetric plate impacts. A Plexiglas window was pressed against the peaks of the perturbation, keeping valleys as free surfaces. This produced perturbation amplitude changes much larger than those predicted without the window. Amplitude reductions from 64 to 88% were measured in recovered samples and grains oriented close to 〈0 0 1〉 parallel to the shock had the largest final amplitude, whereas grains with shocks directions close to 〈1 0 1〉 had the lowest. Finite element simulations were performed with elastic-perfectly plastic models to estimate yield strengths leading lead to those final amplitudes. Anisotropic elasticity and these yield strengths were used to calculate the resolved shear stresses at yielding for the two orientations. Results are compared with reports on orientation dependence of dynamic yielding in Cu single crystals and the higher values obtained suggest that strength estimations via hydrodynamic instabilities are sensitive to strain hardening and strain rate effects.     

Temperature dependence of the stacking fault energy of glide set-dissociated dislocations in Si

Recently, it was found that the dissociation distance of glide set dislocations introduced in Si just below the melting temperature by laser shock peening (LSP) is unusually wide (Iwata et al. J. Jpn Inst. Met. Materi.,79(2015),308–314). In order to distinguish whether or not this is to be attributed to uncorrelated motions of leading and trailing Shockley partials or the intrinsic temperature dependence of the stacking fault energy (SFE), dislocations introduced by LSP were annealed at 1350 °C, which should be high enough for the uncorrelated partials to assume the equilibrium correlated configuration. It was found that this unusual widening of a dissociated dislocation introduced by LSP is attributed to uncorrelated motions of Shockley partials. Following this conclusion, the temperature dependence of the intrinsic SFE of Si was determined on correlated dissociated dislocations up to near the melting temperature. The intrinsic SFE of Si shows only negligibly small temperature dependence from 400 °C up to near the melting temperature.     

The arms of the chimeras

This article seeks to compare the approach developed in 1974 by Michel de M'Uzan to the concept of the ‘chimera’ with Thomas Ogden's ( 1995 , 2005 ) reflections on ‘the analytic third’. This comparison shows that in spite of the different theoretical approaches, unconscious to unconscious communication – a subject of interest in contemporary psychoanalytic research – makes it possible to grasp the intersubjective data deployed in the field of the session. After reviewing M. de M'Uzan's conception of the ‘chimera’ – a product of the unconsciouses of patient and analyst alike, and which emerges during a process of depersonalization in the analyst – the author proposes her hypothesis of the chimera as a particular intersubjective third whose creation, in a hallucinatory state, makes it possible to gain access to the bodily and emotional basis of the trauma. The author describes the chimera as a mental ‘squiggle’ between the two members of the pair which finds expression in different forms; further, she considers that the chimera that seizes the analyst is underpinned by the unconscious affinities of traumatic zones in both protagonists, which permit the grounding, configuration and sharing of the territories of suffering, as apprehended in this paper.     

ASSESSING THE ROLE OF EFFORT REDUCTION IN THE REINFORCING EFFICACY OF TIMEOUT FROM AVOIDANCE

Rats responded on concurrent schedules of shock‐postponement or deletion (avoidance) and timeout from avoidance. In Experiment 1, 3 rats' responses on one lever postponed shocks for 20 s and responses on a second lever produced a 1‐min timeout according to a variable‐interval 45‐s schedule. Across conditions, a warning signal (white noise) was presented 19.5 s, 16 s, 12 s, 8 s, or 4 s before an impending shock. Raising the duration of the warning signal increased both avoidance and timeout response rates. Timeout responding, although positively correlated with avoidance responding, was not correlated with the prevailing shock rate. In Experiment 2, 3 rats' responses on one lever deleted scheduled shocks according to a variable‐cycle 30‐s schedule and responses on a second lever produced a 2‐min timeout as described above. After this baseline condition, the avoidance lever was removed and noncontingent shocks were delivered at intervals yoked to the receipt of shocks in the baseline sessions. Timeout responding decreased when the avoidance lever was removed, even though the shock‐frequency reduction afforded by the timeout remained constant. These results suggest that a key factor in the reinforcing efficacy of timeout is suspension of the requirement to work to avoid shock, rather than the reduction in shock frequency associated with timeout.     

Some fear-potentiating effects of fluprazine hydrochloride in mice

The fear-potentiating effects of fluprazine hydrochloride were explored in a series of five experiments. The drug was found to strongly inhibit entry into a novel open field, exploration of a complex maze, or approach to a novel object placed in the animal's home cage. Time sampling techniques were used to assess drug effects on frequencies of immobility, activity, and grooming and rearing responses prior to and for 70 min after footshock. There were no group differences during the 10 min immediately following shock but the groups diverged steadily in immobility, activity, and grooming for the remainder of the observations. Post-shock activity and grooming responses increased steadily towards baseline values among control mice but remained at low levels or declined among drug-treated mice. Immobility responses declined steadily among control mice but increased substantially among the fluprazine-treated mice throughout post-shock observations. Pairs of male mice treated with fluprazine were also found to maintain greater intermouse distances in a novel linear runway than pairs of undrugged adult males, juvenile males, or male-female pairs. It is suggested that fluprazine hydrochloride both potentiates fear and alters its time course in addition to interfering in some way with normal olfactory processes.     

Withdrawal,matching, withdrawal-matching,and variable-matching strategies in reducing attack-instigated aggression

Several studies have investigated strategies that a participant in dyadic aggression may use to reduce the aggression of the other participant. In one set of these studies the subject is instigated to aggression by an opponent who sets maximum shocks for the subject to receive during the first block of six trials. Following attack-instigation, opponents shift to withdrawal and matching strategies for 18 trials. The withdrawal strategy is an abrupt shift to the lowest levels of shock possible. The matching strategy is the exact matching by an opponent of the shock set by the subject on the previous trial. With these procedures the withdrawal strategy has been the most effective method to reduce aggression. These results led to the suggestion that the effectiveness of the withdrawal strategy may be due to immediate, unambiguous communication by the opponent of his willingness to reduce attack. This hypothesis was tested in the present study by creating a matching condition modified to include immediate, unambiguous communication of willingness to reduce attack. In this withdrawal-matching condition the opponent followed attack with two trials of the lowest levels of shock before shifting to a matching strategy. Although the withdrawal-matching strategy did not lead to shock settings that were significantly lower than the matching strategy, there was evidence that subjects interacting with withdrawal-matching opponents did reduce their shock settings from block 1 to block 4 more rapidly than subjects interacting with matching opponents. A variable matching strategy was also used to provide a more realistic analogy of matching in the mundane world. While subjects with variable-matching opponents also did not set shock levels during blocks 2, 3, and 4 that were significantly lower than the matching strategy, there was evidence that subjects in this condition reduced their shock levels more rapidly than subjects with matching opponents. Although the effects were not as strong as expected, the results do provide some support for the interpretation that the effectiveness of the withdrawal strategy may be due to unambiguous communication of willingness by the opponent to reduce his or her aggression. The effectiveness of the variable-matching strategy was attributed to interrupting the tendency of the subject and his or her opponent to match each other's responses. Consistent with earlier attack-instigated aggression studies, the withdrawal strategy in the present study led to a rapid reduction in aggression.     

Preference for signaled over unsignaled shock schedules: Ruling out asymmetry and response fixation as factors

Experiment 1 tested whether a “symmetrical” choice procedure yields results different from those previously reported using the “unidirectional” standard changeover procedure (e.g., Badia & Culbertson, 1972). Subjects could change at any time from unsignaled to signaled shock by pressing a lever and from signaled to unsignaled shock by pressing a second lever. Results were identical to those of the standard procedure and showed that the standard procedure is fully adequate. Experiment 2 tested whether choice of high density signaled shock over low-density unsignaled shock (Badia, Coker, & Harsh, 1973) resulted from initial training with equal-density schedules. Subjects were trained and tested with signaled shock twice as dense as unsignaled shock. Three of four subjects strongly preferred the signaled condition, thus ruling out carry-over and “response fixation” as alternative explanations.     

The influence of the victim on shock induced aggression in rats.

In two studies, free-roaming male rats (aggressors) were shocked in the presence of male target rats restrained in either an upright or a supine posure. In addition, in Experiment II, two levels of aggressor shock intensity (0.8 mA or 2.0 mA) were used while targets received one of three levels of shock (0.5 mA, 1.5 mA, or 2.5 mA). In both studies, upright targets were attacked less than supine targets. Frequency of aggression was directly related to level of aggressor shock intensity in Experiment II. Also, attack by 0.8-mA aggressors against supine targets was inversely related to level of target shock intensity. The low level of attack against upright targets was interpreted in terms of a threat diaplay. Similarily, it was concluded that the target shock-intensity effect in Experiment II was due to specific threat behaviors displayed by those supine rats that received the highest-intensity shocks.