肿瘤干细胞分离方法研究现状

肿瘤干细胞理论能够解释肿瘤的发生、发展和转移等生物学行为,使人们对恶性肿瘤有一个全新的认识,为临床治疗展现全新视角,使肿瘤靶向治疗找到了新靶点,改变目前药物针对全部癌细胞的治疗策略。本文就肿瘤干细胞分离方法做一简要综述。     

记忆过程中海马CA1区神经元的集群放电特征

观察空间工作记忆过程中海马CA1区神经元群的放电特征。应用多通道神经元集群放电记录技术, 同步观察和记录清醒大鼠在执行延迟选择任务时的行为轨迹以及海马CA1区神经元的放电活动。发现：海马CA1区位置细胞的位置野是在学习过程中逐渐形成并可消退; 部分位置细胞的放电对未来目标定向性行为具有预测作用; 在空间工作记忆过程中, 神经元放电之间的相关性加强, 神经元之间以及神经元与局部场电位之间存在相位编码方式。结果提示海马CA1区神经元参与对空间信息的初级编码和加工, 并为未来行为决策提供有效信息, 而且海马对信息的加工是通过局部神经网络进行, 时间编码可能是海马信息加工的重要方式之一。     

Evaluating motion detection to score sleep disturbance for children: A translational approach to developing a measurement system

Information obtained via direct observation of children's sleep disturbance throughout the night in their home can guide the assessment and treatment process, but watching live or via recorded video might be impractical in terms of time expenditures. In Experiment 1, we assessed the accuracy and reliability of a motion‐detection camera with human motor movements. In Experiment 2, we tested the system's generality by using it to obtain in‐home measures of sleep disturbance for three children with autism spectrum disorder and compared the accuracy to a continuous measurement system. We also measured scoring efficiency and assessed parents' acceptability of the camera. Results provide preliminary support for motion detection in measuring sleep disturbance, but further evaluation of motion detection to improve accuracy is warranted.     

Quantifying false positives in simulated events using partial interval recording and momentary time sampling with dual‐criteria methods

The dual‐criteria (DC) and conservative dual‐criteria (CDC) methods allow clinicians and researchers to quantify the occurrence of false‐positive outcomes within single‐case experimental designs. The purpose of the current study was to use these DC and CDC methods to measure the incidence of false positives with simulated data collected via discontinuous interval methods (i.e., momentary time sampling, partial‐interval recording) as a function of data series length. We generated event data to create 316,800 unique simulated data series for analysis. In Experiment 1, we evaluated how changes in relevant parameters (i.e., interval sizes, event durations, IRT‐to‐event‐run ratios) produced false positives with momentary time sampling procedures. We also assessed the degree that the CDC method produced fewer false positives than the DC method with simulated interval data. In Experiment 2, we used similar procedures to quantify the occurrence of false positives with partial‐interval recording data. We successfully replicated outcomes from previous research in the current study, though such results only highlight the generality of the procedures relating to false positive (and not false negative) outcomes. That is, these results indicate MTS and PIR may adequately control for false positives, but our conclusions are limited by a lack of data on power.     

A Review of: “Barkley,R. A., & Robin,A. L. (2008). Your Defiant Teen: 10 Steps to Resolve Conflict and Rebuild Your Relationship.”

We report a study that addressed behavior data recording by direct-care staff at a child services program. In response to a decreasing percentage of daily data recording in two community-based group homes, a supervisory intervention was implemented, consisting of a revised data recording form and providing performance feedback to the direct-care staff. The intervention increased and maintained the percentage of daily data recording each month across the two group homes and seven sets of child behavior data. The study represents one approach toward staff performance improvement within child services programs.     

BEHAVIORAL COMMUNITY INTERVENTION TO REDUCE THE RISK OF SKIN CANCER

Peer leader modeling, posted feedback, posted goals, and a commitment raffle were used at two swimming pools to increase behaviors associated with skin cancer prevention. During the intervention condition, pool lifeguards modeled the protective behaviors by wearing sunglasses, t-shirts, and hats, using zinc oxide and sunscreen, and staying in the shade. Children and adolescents (1 to 16 years old) increased their use of two or more protective behaviors from a baseline mean of 6.5% to 26.9% during the intervention. Adults (older than 16 years) increased their protective behaviors from a baseline mean of 22% to 37.95% during the intervention. The lifeguards increased their use of all the protective behaviors from a baseline mean of 16.7% to 63.5% during intervention. Ways to improve and expand this intervention are discussed.     

SOCIAL-SKILLS TRAINING TO MODIFY ABUSIVE VERBAL OUTBURSTS IN ADULTS

Social-skills training was used to modify abusive verbal outbursts displayed by two adult psychiatric patients. Five target behaviors—looking, irrelevant comments, hostile comments, inappropriate requests, and appropriate requests were monitored during role-played situations. Social-skills training, consisting of behavior rehearsal with modelling, focused instructions, and feedback, was introduced in a multiple-baseline design across individuals. Training improved all target behaviors. The improved behavior generalized to: (1) novel scenes role-played with the original respondent, (2) training and novel scenes role-played with a different respondent, and (3) interpersonal situations on the hospital ward.     

A REVIEW OF ACADEMIC PERMANENT-PRODUCT DATA COLLECTION AND RELIABILITY PROCEDURES IN APPLIED BEHAVIOR ANALYSIS RESEARCH1

Three publication sources were reviewed to determine the recent conventions for collecting, and assessing the reliability of, academic permanent-product data (handwriting, examination papers, etc in applied behavior analysis. The primary source was the Journal of Applied Behavior Analysis (1968–1974). Secondary sources included conference proceedings titled, A new direction in education: behavior analysis (E. Ramp and B. L. Hopkins, Eds., Lawrence, Kansas: Support and Development Center for Follow Through, Department of Human Development, University of Kansas, 1971), and Behavior Analysis and Education (G. Semb, Ed., Lawrence, Kansas: Support and Development Center for Follow Through, Department of Human Development, University of Kansas, 1972). Finally, as a test of the generality of the findings in the two applied behavior analysis sources, the current issue of each of 14 psychological and/or educational journals was reviewed. Thirty JABA studies reported academic permanent-product data, but only 14 reported reliability. Increasingly more product data through 1973 have been reported along with a greater proportion of authors reporting reliability. The review of the two conference proceeding publications revealed the same trend. In 1971, only three studies reported academic product data, none with reliability, while in 1972, 15 reported academic data, nine including reliability assessment. The review of 14 current education/psychology journal issues revealed four studies reporting academic data, none with reliability. Across all sources, about one-half of the studies reported reliability. Most of the studies reporting reliability described the frequency of reliability assessment, with approximately equal numbers of JABA studies reporting reliability for each paper or reliability for each session. The use of uninformed observers was reported in only three JABA studies and one conference study. Marks made on subjects' papers by either the teacher or the primary observer were reported masked for reliability purposes by only two JABA and two conference studies. Reliability was calculated on a session total basis in two JABA studies. Point-by-point agreement was given in nine JABA and three conference studies. Perfect reliability (mean agreement of 100%) was reported in only six JABA and three conference studies. Scores between 90 and 100% were reported in nine JABA and four conference studies. Scores below 80% were reported in three JABA studies. No other percentage agreement scores were reported, although one JABA study reported correlational reliability (Pearson r). In summary, recently more studies have dealt with academic data and, until 1974, a greater proportion of these studies reported reliability assessment, and relatively few studies reported either replicable methods, 100% agreement, or controls for maintaining rater independence.     

AN EXPERIMENTAL ANALYSIS OF “SPILLOVER” EFFECTS ON THE SOCIAL INTERACTION OF BEHAVIORALLY HANDICAPPED PRESCHOOL CHILDRENsp1

The effects of prompting and social reinforcement directed to target subjects on their social behavior and that of peers who never received prompting and reinforcement for positive social behavior, were examined. In a combined reversal and multiple-baseline design, three behaviorally handicapped preschool boys who exhibited divergent social behavior repertoires and varied histories with social reinforcement events, were sequentially exposed to intervention conditions in order to investigate “spillover” of treatment effects. Prompting and reinforcement increased positive social behavior and decreased negative social behavior emitted by all target subjects. The results also demonstrated a “spillover” effect on two target subjects, who at various times were not under intervention, and on the peers as well. The findings suggest that: (a) the direct and indirect effects of intervention procedures may be enhanced by designing treatment based on the social repertoire and reinforcement histories of the subjects; and (b) the treatment “spillover” effect may be increased by applying procedures to two children at once, rather than to one at a time.