条件性恐惧大鼠边缘下区Cdk5激酶活性、caspase-3表达以及突触结构的变化

探讨条件性恐惧大鼠内侧前额叶皮层边缘下区(IL区)Cdk5激酶活性、凋亡因子caspase-3表达和突触结构的变化。采用声音结合足底电击的方法对大鼠建立条件性恐惧模型后, 在不同时间点测定IL区Cdk5激活剂P35和P25的蛋白表达水平、Cdk5激酶活性、caspase-3的阳性细胞数, 并观察突触结构的变化。结果发现, 大鼠在条件性恐惧建立后第2、4和8天, IL区的P25蛋白表达和Cdk5激酶活性均明显高于正常; caspase-3的免疫反应阳性细胞数在3个时间点也均多于正常; 透射电镜下观察到：在恐惧建立后的第8天, IL区突触的突触后致密物质(PSD)厚度变薄, 在恐惧建立后第22天突触数密度减少、PSD厚度仍小于正常。推测条件性恐惧的建立, 使大鼠IL区的P25水平和Cdk5活性高于正常, 引起IL区的突触结构发生改变, 导致大鼠的恐惧反应持续存在。     

条件反射性免疫抑制研究的新进展

条件反射性免疫抑制 (CIS)模式基于巴甫洛夫条件反射的机理 ,通过大脑对免疫系统进行调节 ,是一种重要的心理神经免疫学方法。近 10年来的研究取得了一些重要的进展。首先 ,条件反射性免疫抑制模型已证实是非常稳定可靠的心理神经免疫模型。其次 ,关于CIS的机制在体液、细胞、神经学方面都有广泛的研究 ,而近年来更趋向于对神经性通路的探讨。而CIS的临床应用性研究也在实验动物身上取得了稳定可靠的结果 ,证实了其未来临床应用的可行性及潜力     

银屑病的发病机制：天然免疫的启示

银屑病是一种常见的炎症性皮肤病,属于自身免疫病的范畴。天然免疫对保护机体免受微生物感染具有重要作用,同时天然免疫在过度激活的情况下又能够导致自身免疫病。银屑病与天然免疫功能紊乱有着密切的关系。只有充分的发挥创造性思维,密切结合临床特征,才能从天然免疫中寻找银屑病发病的相关证据。     

吗啡行为及条件性行为敏感化效应及其个体差异

目的：考察吗啡处理下,大鼠行为敏感化及条件性行为敏感化效应及其个体差异性表现。方法：根据大鼠在初次抵达的新颖环境中水平活动量的高低,将大鼠划分为高反应大鼠（High responder, HR）和低反应大鼠（Low responder, LR）,应用自动监测大鼠活动箱,分别考察HR和LR大鼠在行为及条件性行为敏感化效应表达上的差异。结果：（1）连续5天吗啡给药,LR大鼠活动量显著升高,HR大鼠无此效应;（2）条件测试日（第6天）,给药与环境匹配大鼠,活动量较给药与环境非匹配组动物和对照组动物显著为高;此效应在HR和LR大鼠同时存在;（3）从给予吗啡到给予盐水,发现LR大鼠活动量显著下降,而HR大鼠活动量无显著改变。结论：在连续给药下,LR大鼠较HR大鼠,在行为敏感化效应的形成中,具有更为显著的效应,此效应为LR动物对吗啡更高的药物效应,而非条件效应所致,同时HR和LR大鼠都可以对吗啡条件性线索产生应答,产生条件性行为敏感化效应。     

Further Evidence that Social Interaction Reduces Conditioned Taste Aversions in Mice

Social interactions with other conspecifics affect food selection in mammals. A previous study suggested that male mice attenuate conditioned taste aversion (CTA) by lithium chloride after interacting with an unfamiliar male conspecific. The present study was designed to (a) examine whether interactions with a familiar conspecific would attenuate CTA and (b) investigate sex differences in the social attenuation of CTA in mice. Results indicated that interacting with a familiar conspecific of the same sex following poisoning attenuated conditioned taste aversions only in male mice. The results suggest that the familiarity of conspecifics was not a critical factor in the social attenuation of CTA. Discrepancies between present and previous findings are discussed.     

心理神经免疫学的研究及其思路问题

回顾并分析了心理神经免疫学的研究历史和发展,指出要深入揭示行为、精神活动是如何转化为影响健康的生理状态的奥秘,需要新的研究思路并讨论了新的研究路线     

Mousekilling,intermale fighting,and conditioned emotional response in rats

Several possible relationships between two forms of aggression in rats were studied. First, mouse killing and spontaneous intermale fighting were found to be correlated. Rats which attacked other rats were those most likely to kill mice. To determine whether aggressive and nonaggressive rats were also differentially responsive to other situations involving emotional arousal, but not aggression, mouse-killers and nonkillers were compared in a conditioned emotional response (CER) situation. Mouse-killers showed greater suppression to the conditioned stimulus (CS) and to the situational cues of the apparatus. Therefore, a common arousal mechanism may underlie a number of diverse agonistic responses. Nevertheless, extensive mouse-killing experience did not increase the tendency of rats to fight with either adult males or juvenile males.     

Variability and creativity in small-sided conditioned games among elite soccer players

ObjectiveSmall-sided conditioned games (SSCG) in soccer are games with a small number of players, often played on smaller than regular pitches and with adapted rules. It has been argued that SSCG foster soccer players’ physical, technical and tactical performances and creativity. This study tested the latter conjecture by analysing video-footage of individual actions of elite soccer players in 5 v 5, 6 v 6, 7 v 7 SSCG played during regular training sessions and 11-aside training matches. Based on the ecological dynamics approach, we hypothesized that smaller formats would result in players making more individual actions. We additionally anticipated that the smaller formats players would induce a larger repertoire of actions, that is, an increased variability of actions, and that such increase in variability would be associated with more creative actions. Along the same lines, we reasoned that midfielders would make more creative actions than defenders and attackers.MethodWe categorized 3555 soccer actions on the ball and without the ball of 24 elite soccer players.ResultsPlayers produced more actions in smaller SSCG formats compared to the larger SSCG format and the 11-aside match. They also produced more different actions in SSCG than the 11-aside match. Furthermore, ten creative actions (i.e., actions that were adequate and only made by one or two players) were discerned. The creative actions emerged most often in the smaller SSCG, and were absent in the 11-aside matches. Finally, strikers, defenders and midfielders did not show reliable differences in terms of number, variability and creativity of action.ConclusionSSCG in soccer do indeed stimulate variability and creativity of individual actions. It is important to confirm whether these immediate effects of SSCG generalize across longer time scales.