GIRK1在宫颈癌组织细胞中的表达

探求内向整流型钾离子通道1（GIRK1）与宫颈癌发生发展的关系.利用RT-PCR及免疫组织化学技术检测GIRK1 mRNA及蛋白在15例正常宫颈和30例宫颈癌组织细胞中的表达.GIRK1 mRNA在宫颈癌组织中的表达高于在正常宫颈组织中的表达,差异有统计学意义（P〈0.01）;GIRK1蛋白在宫颈癌组织中的阳性表达率为100%,在正常宫颈组织中的阳性表达率为20%,两者差异有统计学意义（P〈0.01）.GIRK1 mRNA及蛋白在宫颈癌组织中呈高表达,可能与宫颈癌的发生及发展有关.     

严格掌握使用粒细胞集落刺激因子的适应证

粒细胞集落刺激因子（G—CSF）可以作用于粒系前体造血干细胞和外周成熟的效益细胞,促进其增殖、分化。G—CSF促使组织修复和血管生成的效能使其在临床上得到广泛使用,与此同时,G—CSF及受体在肿瘤中的表达及该药物的不良反应也引起了关注,本文旨在探讨G—CSF功效及副作用,引起临床医师的重视,把握适应证,指导合理用药。     

关于内科临床技能培训教学课程设置的探讨

通过对参加内科临床技能培训的大连医科大学2007级、2009级五年制及七年制临床医学专业学生进行问卷调查及客观结构化临床考试（OSCE）,探讨内科临床技能培训课程的学时及课程内容等设置.100%的学生认为有开设临床技能培训课的必要,50%以上的学生认为在大学4年级开设本课程较合适,50%以上的学生认为12学时的设置较合理,80%以上的学生认为应每4人共用一名模拟人.OSCE证实毕业前12学时培训组成绩优于实习前8学时组.     

Family structure and family processes in Mexican-American families

Despite increases in single-parent families among Mexican Americans, few studies have examined the association of family structure and family adjustment. Utilizing a diverse sample of 738 Mexican-American families (21.7% single parent), the current study examined differences across family structure on early adolescent outcomes, family functioning, and parent-child relationship variables. Results revealed that early adolescents in single-parent families reported greater school misconduct, conduct disorder/oppositional deviant disorder, and major depressive disorder symptoms, and greater parent-child conflict than their counterparts in 2-parent families. Single-parent mothers reported greater economic hardship, depression, and family stress. Family stress and parent-child conflict emerged as significant mediators of the association between family structure and early adolescent outcomes, suggesting important processes linking Mexican-American single-parent families and adolescent adjustment.     

Cerebellar-induced apraxic agraphia: a review and three new cases

Apraxic agraphia is a writing disorder due to a loss or lack of access to motor engrams that program the movements necessary to produce letters. Clinical and functional neuroimaging studies have demonstrated that the neural network responsible for writing includes the superior parietal region and the dorsolateral and medial premotor cortex. Recent studies of two cases with atypical lesion localisations in the left thalamus and the right cerebellum support the hypothesis that the written language network is larger than previously assumed. The aim of this study is twofold: (1) to provide a survey of cases of apraxic agraphia published between 1973 and June 2010, and (2) to provide further evidence for a role of the cerebellum in writing via three additional cases who presented with apraxic agraphia after ischemic damage in the cerebellum. Functional neuroimaging studies by means of brain perfusion SPECT showed perfusional deficits in the anatomoclinically suspected supratentorial areas, subserving language dynamics, syntax, naming, writing and executive functioning.     

ICU向患者家属开放的理论研究

ICU实行的基本是封闭式管理,这不仅带来了一些伦理问题,也容易引发医疗纠纷,从伦理学的需要、预防医疗纠纷、改善医患关系等方面探讨ICU向患者家属开放的必要性,分析开放存在的问题,论证了ICU向患者家属开放的可行性。     

Position of the non-stance leg during the single leg squat affects females and males differently

BackgroundKinematic differences between females and males for the single leg squat (SLS) have been identified. However, kinetic differences between sexes and how variations of the non-stance leg position during the SLS may affect kinematics and kinetics differently in females and males have not been examined.ObjectivesExamine sex-specific kinematic and kinetic differences during the SLS task with 3 different non-stance leg positions.DesignControlled laboratory study, cross-sectional design.MethodsThirty-two healthy adults (16 females, 16 males) performed the 3 SLS tasks while data were collected using a motion capture system and force plates. At 60 degrees of knee flexion (60KF) and peak knee flexion (PKF), kinematics and joint moments were compared between sexes and SLS tasks using a linear regression analysis.ResultsFemales exhibited less ipsilateral trunk flexion (P < 0.001) and greater anterior pelvic tilt (P ≤ 0.021) and hip adduction (P < 0.001) than males across tasks at 60KF and PKF. Across tasks, females had a smaller knee flexion moment than males at PKF (P = 0.001). Females had a greater hip abduction moment during SLS-Front than SLS-Middle (P = 0.044) and SLS-Back (P = 0.003) at PKF, but males had similar hip abduction moments across tasks (P ≥ 0.299). At 60KF, males had a greater knee adduction moment during SLS-Front compared to the other tasks (P ≤ 0.019) while females had similar hip abduction moments across tasks (P ≥ 0.459).ConclusionAltering the non-stance leg position during the SLS affects the kinematics and kinetics of both females and males. The position of the non-stance leg can be modified for assessment and treatment purposes and should be reported in research.     

Single leg landing movement differences between male and female badminton players after overhead stroke in the backhand-side court

Females showed higher anterior cruciate ligament (ACL) injuries rate on the opposite side of dominant hand compared with males during single leg landing in the backhand-side court after overhead stroke. The purpose of this study was to conduct biomechanics testing including kinematics and kinetics to provide some insights on the ACL injuries risks during single leg landing in the backhand-side court after overhead stroke between females and males. Twenty collegiate badminton players (10 females, 10 males) voluntarily participated in this study. Sagittal plane kinematic and kinetic data of the lower limb, and their ground reaction forces during the single leg landing in the backhand-side court after overhead stroke were collected. Results shown that, at the peak posterior ground reaction force (GRF) moment, the ankle dorsiflexion, knee and hip flexion angles of the female were lower than that of male. Meantime, the knee extension moment of the female was lower than that of males but the hip extension moment of the female was larger compared to males at the peak posterior GRF moment. The peak vertical and posterior GRF of female badminton players were larger than that of males. Decreased hip, knee, and ankle flexion angles at the peak posterior GRF moment and greater peak vertical and posterior GRF may expose female badminton players to the higher risk ACL injuries compared to males during single leg landing after overhead stroke in the backcourt-side. Preventative training programs designed to prevent the ACL injuries rate of female badminton players should take these factors into consideration.     

Neuromuscular control in individuals with chronic ankle instability: A comparison of unexpected and expected ankle inversion perturbations during a single leg drop-landing

While neuromuscular control deficits during inversion perturbations in chronic ankle instability (CAI) cohorts are well documented in the literature, anticipatory motor control strategies to inversion perturbations in CAI are largely unknown. The purpose of this study was to examine neuromuscular control and ankle kinematics in individuals with CAI (n = 15) and matched controls (n = 15) during unexpected and expected single leg drop-landings onto a tilted surface rotated 20° in the frontal plane. Muscle activity from 200 ms pre- to post-landing was recorded from the tibialis anterior (TA), medial gastrocnemius (MG), peroneus longus (PL) and peroneus brevis (PB). Mean muscle activity, co-contraction index (CCI), and peroneal latency was analyzed. Ankle inversion angle at initial contact, time to maximum inversion angle, maximum inversion angle and velocity were also assessed. Significantly longer PL latency, less time to maximum inversion and greater maximum inversion angle was found in CAI compared to controls. Regarding landing condition, significantly greater maximum inversion angle, less inversion at initial contact, longer PB latency, less TA activity and frontal plane CCI during the post-landing phase was found during the unexpected perturbation. Prolonged PL latency and altered ankle kinematics suggests reduced frontal plane ankle stabilization in CAI. However, similar motor control strategies were utilized in both groups during the ankle inversion perturbations.     

Central executive function in mild cognitive impairment: a PET activation study

Using positron emission tomography (PET), we explored the neural correlates of an executive function, dual tasking, in patients with amnestic mild cognitive impairment (aMCI) and in elderly controls. The experiment employed simple auditory and visual tasks that were presented both in isolation and simultaneously to create a task condition requiring enhanced attentional control. Behaviorally, both groups performed well, albeit the patients made more errors on the visual task. The PET analysis focused at prefrontal regions where group differences in task-related activation patterns were expected. During dual task performance, the patients showed attenuated activity in the left inferior frontal region when compared to the controls. This suggests abnormalities in the neural processes underlying attentional control in aMCI.