Inhibition of the LH Surge by Restraint Stress in Cyclic Rats: Studies on the Role of GABAA and GABAB Receptors

There is evidence that stress can alter the activity in the brain of gamma-aminobutyricacid (GABA), a neurotransmitter that has been implicated in the regulation of LH secretion. In the present study the role of GABA in the restraint stress-induced inhibition of the LH surge was investigated in the intact cyclic rat. Intracerebroventricular (icv) administration of the GABAA receptor agonist muscimol (0.1, 0.5 or 1 μg) 5 min before the presumed onset of the pro-oestrous LH surge (at 0900 h) caused a dose dependent suppression of the surge. A single dose of the GABAB receptor agonist baclofen (1 μg; icv) injected at 0855 h postponed the onset of the LH surge, and repeated injections at 0855 and 1130 h suppressed the surge. These data indicate that GABA-ergic activity in the brain can inhibit the LH surge in the cyclic rat via GABAA and GABAB receptors. Pro-oestrous rats were subjected to 5 hrs of restraint starting at 0855 h. Pretreatment with the GABAA receptor antagonist bicuculine (1 μg; icv) at 0840, 0940 and 1040 h or pretreatment with the GABAB receptor antagonist phaclofen (10 μg; icv) at 0840 h were ineffective in preventing the restraint-induced inhibition of the LH surge. The results suggest that GABAA and GABAB receptors are not involved in the inhibitory effect of restraint stress on the LH surge.     

Mini-Review; Stress Genes: An Introductory Overview

Molecular sequence data, made available in the last 15 years or so, have led to the classification of living cells into three phylogenetic domains: Bacteria, Archaea, and Eucarya. All the organisms that have been tested belonging to either domain were capable of mounting a stress response with essentially the same characteristics, regardless of the stressor. The protagonists in the cell's stress response are the stress genes and their protein products. Some of the latter are molecular chaperones. Under physiological conditions, these chaperones aid other cellular proteins to fold properly and achieve a native -functional- configuration, and to translocate from the place of synthesis to the cell's locale in which they will operate. In a stressed cell, the stress proteins that are chaperones protect other molecules from denaturation and help those partially damaged to regain a functional configuration. Thus, cell death is avoided and recovery is enhanced. The study of stress genes and proteins has progressed considerably in organisms belonging to the domains Bacteria and Eucarya. Less is known about the archaeal stress genes. Here, research with an organism from the Archaea is discussed, focusing on the stress genes of the hsp70 (dnaK) locus. Future perspectives for basic and applied research within the health sciences and biotechnology industries are presented.     

Measurement and the interpretation of quantum mechanics and relativity theory

The axiomatic approaches of quantum mechanics and relativity theory are compared with approaches in which the theories are thought to describe readings of certain measurement operations. The usual axioms are shown to correspond with classes of ideal measurements. The necessity is discussed of generalizing the formalisms of both quantum mechanics and relativity theory so as to encompass more realistic nonideal measurements. It is argued that this generalization favours an empiricist interpretation of the mathematical formalisms over a realist one.     

A simple method using a single video camera to determine the three-dimensional position of a fish

In the present paper, we describe a method for recording the coordinates of a fish in an aquarium in a three-dimensional space, using a single video camera and a mirror. We use photogrammetic equations for this, considering the image obtained in the mirror as a virtual image obtained by a second camera. A transformation of the coordinate system is required to express the obtained coordinates in anx,y,z system defined by the edges of the aquarium. The accuracy of the proposed method was estimated, and errors in extreme conditions were found to be 0.8% to 1.2 %, compared with the dimensions of the aquarium used in the test.     

Intuitive Perceptions of Decision-making Strategy: Naive Assessors' Concepts of Integrative Complexity

Although there are measurable differences in integrative complexity among solutions that individuals generate in dealing with problems, it is uncertain to what extent people comprehend, recognize, and have preferences among different levels of complexity. Integrative complexity is a function of differentiation (the perception of several attributes within, or perspectives about, a topic) and integration (combining the differentiated characteristics in an interactive or synthesizing solution). The current paper reports two experiments dealing with how university students perceive, interpret, and choose among solutions differing in complexity. Experiment 1 showed that subjects accurately rated the complexity of described solutions differing along the continuum, but that their assessment of their own responses differed from the results of expert scoring. Their self-estimated complexity was highly correlated with their preferences, and preferred complexity was reliably higher than either expert- or self-assessed complexity of subject-generated solutions. Subjects were able to hypothesize quite accurately about environmental and endogenous factors likely to affect complexity. Experiment 2 found that in response to problem scenarios, solutions selected as being potentially most effective were consistently more complex than solutions that participants considered themselves most likely to use. The idea of complexity seems to be intuitively recognizable and understandable by untrained subjects: They can and do distinguish among problem solutions (self-generated or presented) that vary on that dimension, and are able to assess accurately the effects of relevant variables. Such subjects also share the bias shown by experts in favour of the superiority of more complex approaches.     

Validation of a full body 3-D dynamic linked segment model

In studies related to human movement, linked segment models (LSM's) are often used to quantify forces and torques, generated in body joints. Some LSM's represent only a few body segments. Others, for instance used in studies on the control of whole body movements, include all body segments. As a consequence of the complexity of 3-dimensional (3-D) analyses, most LSM's are restricted to one plane of motion. However, in asymmetric movements this may result in a loss of relevant information. The aim of the current study was to develop and validate a 3-D LSM including all body segments. Braces with markers, attached to all body segments, were used to record the body movements. The validation of the model was accomplished by comparing the measured with the estimated ground reaction force and by comparing the torques at the lumbo-sacral joint that resulted from a bottom-up and a top-down mechanical analysis. For both comparisons, reasonable to good agreement was found. Sources of error that could not be analysed this way, were subjected to an additional sensitivity analysis. It was concluded that the internal validity of the current model is quite satisfactory.     

Meaning,amount of processing,and memory for words

Two experiments were carried out in order to try to resolve the controversy about whether the semantic processing of a word necessarily involves all the elements of its meaning. In the first experiment, subjects categorized a list of auditorially presented words according to whether or not they were natural consumable solids (e.g., “apple“). They were then given an unexpected test of their ability to recall the whole list, which contained equal numbers of words with none, one, two, or all three of the target components. The results confirmed the prediction that the greater the number of components a word has in common with a target category, the better it will be recalled. The second experiment used a visual presentation of a list of words, which subjects scanned for members of a particular target category. There were four different target categories assigned to independent groups of subjects. The results again confirmed the prediction. On the assumption that memorability in such tasks is determined by amount of processing, we conclude that some elements of the meaning of a word can be processed without having to process all of them.     

A new computational method to fit the weighted euclidean distance model

This paper describes a computational method for weighted euclidean distance scaling which combines aspects of an analytic solution with an approach using loss functions. We justify this new method by giving a simplified treatment of the algebraic properties of a transformed version of the weighted distance model. The new algorithm is much faster than INDSCAL yet less arbitrary than other analytic procedures. The procedure, which we call SUMSCAL (subjectivemetricscaling), gives essentially the same solutions as INDSCAL for two moderate-size data sets tested.Comments by J. Douglas Carroll and J. B. Kruskal have been very helpful in preparing this paper.