Synaptic Reliability Correlates with Reduced Susceptibility to Synaptic Potentiation by Brain-Derived Neurotrophic Factor

Recent studies have implicated brain-derived neurotrophic factor (BDNF) in use-dependent modification of hippocampal synapses. BDNF can rapidly potentiate synaptic transmission at glutamatergic synapses by enhancing transmitter release. Using simultaneous perforated patch recording from pairs and triplets of glutamatergic hippocampal neurons, we have examined how the initial state of the glutamatergic synapse determines its susceptibility to synaptic modification by BDNF. We found that the degree of synaptic potentiation by BDNF depends on the initial reliability and strength of the synapse: Relatively weak connections were strongly potentiated, whereas the effect was markedly reduced at stronger synapses. The degree of BDNF-induced potentiation strongly correlated with the initial coefficient of variation (CV) of the amplitude of excitatory postsynaptic currents (EPSCs) and inversely correlated with the initial paired–pulse facilitation, suggesting that synapses with lower release probability (P_r) are more susceptible to the action of BDNF. To determine whether saturation of P_r could have masked the potentiation effect of BDNF in the stronger synapses, we lowered the initial P_r either by reducing the extracellular Ca²⁺ concentration ([Ca²⁺]_o) or by bath application of adenosine. Synapses that were initially strong remained unaffected by BDNF under these conditions of reduced P_r. Thus, the lack of BDNF effect on synaptic efficacy cannot simply be accounted for by saturation of P_r, but rather may be due to intrinsic changes associated with synaptic maturation that might covary with P_r. Finally, the dependence on initial synaptic strength was also found for divergent outputs of the same presynaptic neuron, suggesting that synaptic terminals with different degrees of responsiveness to BDNF can coexist within in the same neuron.     

Theoretical Medicine and Bioethics Index to Volume 20

Volume Contents

heoretical Medicine and Bioethics Index to Volume 20     

Drug discrimination under a concurrent fixed-ratio fixed-ratio schedule.

Pigeons were trained to discriminate 5.0 mg/kg pentobarbital from saline under a two-key concurrent fixed-ratio 10 fixed-ratio 40 schedule of food presentation, in which the fixed-ratio component with the lower response requirement was programmed to reinforce responding on one key after drug administration (pentobarbital-biased key) and on the other key after saline administration (saline-biased key). After responding stabilized, pigeons averaged 98% of their responses on the pentobarbital-biased key during training sessions preceded by pentobarbital, and they averaged 90% of their responses on the saline-biased key during training sessions preceded by saline. In test sessions preceded by doses of pentobarbital, chlordiazepoxide, or ethanol, pigeons switched from responding on the saline-biased key at low doses to responding on the pentobarbital-biased key at higher doses (the dose-response curve was quantal). High doses of phencyclidine produced responding on both keys, whereas pigeons responded almost exclusively on the saline-biased key after all doses of methamphetamine. These and previous experiments using concurrent reinforcement schedules to study drug discrimination illustrate that the schedule of reinforcement is an important determinant of the shape of dose-effect curves in drug-discrimination experiments.     

A discrimination analysis of training-structure effects on stimulus equivalence outcomes.

Experiments designed to establish stimulus equivalence classes frequently produce differential outcomes that may be attributable to training structure, defined as the order and arrangement of baseline conditional discrimination training trials. Several possible explanations for these differences have been suggested. Here we develop a hypothesis based on an analysis of the simple simultaneous and successive discriminations embedded in conditional discrimination training and testing within each of the training structures that are typically used in stimulus equivalence experiments. Our analysis shows that only the comparison-as-node (many-to-one) structure presents all the simple discriminations in training that are subsequently required for consistently positive outcomes on all tests for the properties of equivalence. The sample-as-node (one-to-many) training structure does not present all the simple discriminations required for positive outcomes on either the symmetry or combined transitivity and symmetry (equivalence) tests. The linear-series training structure presents all the simple discriminations required for consistently positive outcomes on tests for symmetry, but not for symmetry and transitivity combined (equivalence) or transitivity alone. Further, the difference in the number of simple discriminations presented in comparison-as-node training versus the other training structures is larger when the intended class size is greater than three or the number of classes is larger than two. We discuss the relevance of this analysis to interpretations of stimulus equivalence research, as well as some methodological and theoretical implications.     

One extreme or the other or perhaps the golden mean? Issues of spatial resolution in face processing

The findings of some of the key studies that have examined the contribution of different bands of spatial information, specified in terms of cycles per face, to face recognition, and identification are reviewed. Based on findings from studies of low-, high-, and band-pass filtering, it is concluded that neither low facial scales nor high facial scales are optimal. Instead, a center range of facial scales, approximately 8-16 cycles per face, appears to make the greatest contribution to the recognition process. A study of identification of band-pass filtered faces is reported that reinforces the view derived from the literature. This indicated that a band with a harmonic mean of 11.1 cycles per face provided the most efficient recognition, with speed and accuracy declining at lower and higher bands.     

Counter-stereotypical impulsivity and violence among African American college men

The study examined the relationship between impulse control and violence in terms of past feelings and acts among African American males enrolled in Historically Black Colleges and Universities. Data were gathered from a sample of 1,874 male undergraduates in eleven colleges and universities with predominantly African-American students. Less than six percent were classified as impulsive, and even less than half of the impulsive males had past feelings and acts of violence. The study offers strategies for impulse control which may help to further minimize incidents of violence on the college campus.