Mapping of olfactory memory circuits: region-specific c-fos activation after odor-reward associative learning or after its retrieval

Although there is growing knowledge about intracellular mechanisms underlying neuronal plasticity and memory consolidation and reconsolidation after retrieval, information concerning the interaction among brain areas during formation and retrieval of memory is relatively sparse and fragmented. Addressing this question requires simultaneous monitoring of activity in multiple brain regions during learning, the post-acquisition consolidation period, and retrieval and subsequent reconsolidation. Immunoreaction to the immediate early gene c-fos is a powerful tool to mark neuronal activation of specific populations of neurons. Using this method, we are able to report, for the first time, post-training activation of a network of closely related brain regions, particularly in the frontal cortex and the basolateral amygdala (BLA), that is specific to the learning of an odor-reward association. On the other hand, retrieval of a well-established associative memory trace does not seem to differentially activate the same regions. The amygdala, in particular, is not engaged after retrieval, whereas the lateral habenula (LHab) shows strong activation that is restricted to animals having previously learned the association. Although intracellular mechanisms may be similar during consolidation and reconsolidation, this study indicates that different brain circuits are involved in the two processes, at least with respect to a rapidly learned olfactory task.     

The Relation of Perceived Neighborhood Danger to Childhood Aggression: A Test of Mediating Mechanisms

In the current study, two mediational mechanisms, parenting practices and children's beliefs about aggression, were hypothesized to account for the relationship between perceived neighborhood danger and childhood aggression. Using structural equation modeling, data were analyzed from an inner-city school-based sample of 732 predominantly African American 5th graders. Results suggested that perceived neighborhood danger was associated with strong positive beliefs about aggression, which in turn was associated with high levels of aggression. The hypothesized mediating role of parenting practices (restrictive discipline, parental monitoring, and parental involvement) on the relation between perceived neighborhood danger and child aggression was not supported. However, the current findings suggest that children's positive beliefs about aggression mediated the relationship between restrictive discipline and aggression. Directions for future research are discussed.     

The Process of Family Reconstruction after DNA Testing for Huntington Disease

The needs of families to reconstruct their relationships in response to the DNA testing for Huntington disease of one or more of their asymptomatic members are presented. Data were collected from family interviews with 18 families, and from their responses on a post interview questionnaire. Findings are that families need to (a) address unfinished business associated with the decision for testing; (b) bring family members, peripheral in the decision for testing, into the loop; (c) reorganize patterns of communication and roles altered by the testing and heal ruptures in family membership; and (d) revise family stories about illness to provide a meaning for HD and explain the test results in a way that leaves them with a sense of mastery. Findings suggest that families should be more involved in the initial decision for testing of a member and that protocols should be established to provide help for their ongoing adjustment.     

Cognitive-behavioral therapy for ADHD in medication-treated adults with continued symptoms

The purpose of the present study was to examine the potential efficacy, patient acceptability, and feasibility of a novel, cognitive-behavioral therapy (CBT) for adults with attention-deficit hyperactivity disorder (ADHD) who have been stabilized on medications but still show clinically significant symptoms. Thirty-one adults with ADHD and stable psychopharmacology for ADHD were randomized to CBT plus continued psychopharmacology or continued psychopharmacology alone. Assessments included ADHD severity and associated anxiety and depression rated by an independent evaluator (IE) and by self-report. At the outcome assessment, those who were randomized to CBT had lower IE-rated ADHD symptoms (p < .01) and global severity (p < .002), as well as self-reported ADHD symptoms (p < .0001) than those randomized to continued psychopharmacology alone. Those in the CBT group also had lower IE-rated and self-report anxiety (p's < .04), lower IE-rated depression (p < .01), and a trend to have lower self-reported depression (p = .06). CBT continued to show superiority over continued psychopharmacology alone when statistically controlling levels of depression in analyses of core ADHD symptoms. There were significantly more treatment responders among patients who received CBT (56%) compared to those who did not (13%) (p < .02). These data support the hypothesis that CBT for adults with ADHD with residual symptoms is a feasible, acceptable, and potentially efficacious next-step treatment approach, worthy of further testing.     

On the relation between maternal state of mind and sensitivity in the prediction of infant attachment security

Attachment theorists assume that maternal mental representations influence responsivity, which influences infant attachment security. However, primary studies do not support this mediation model. The authors tested mediation using 2 mother-infant samples and found no evidence of mediation. Therefore, the authors explored sensitivity as a moderator, studying the (a) interaction of mental representation and sensitivity as it predicts infant attachment security and (b) level of sensitivity in mothers whose infants' attachment security is either concordant or discordant with their own. The interactional analyses were not significant. But the match-mismatch data showed that when mother-infant attachment strategies were discordant, maternal sensitivity was more consistent with infant than maternal attachment strategy. These findings are congruent with an interpretation of sensitivity as a moderator that can block transmission of attachment strategy.     

The role of inferences about referential intent in word learning: evidence from autism

Young children are readily able to use known labels to constrain hypotheses about the meanings of new words under conditions of referential ambiguity. At issue is the kind of information children use to constrain such hypotheses. According to one theory, children take into account the speaker's intention when solving a referential puzzle. In the present studies, children with autism were impaired in monitoring referential intent, but were equally successful as normally developing 24-month-old toddlers at mapping novel words to unnamed items under conditions of referential ambiguity. Therefore, constraints that lead the child to map a novel label to a previously unnamed object under these circumstances are not solely based on assessments of speakers' intentions.     

Gesture is at the cutting edge of early language development

Children who produce one word at a time often use gesture to supplement their speech, turning a single word into an utterance that conveys a sentence-like meaning ('eat'+point at cookie). Interestingly, the age at which children first produce supplementary gesture-speech combinations of this sort reliably predicts the age at which they first produce two-word utterances. Gesture thus serves as a signal that a child will soon be ready to begin producing multi-word sentences. The question is what happens next. Gesture could continue to expand a child's communicative repertoire over development, combining with words to convey increasingly complex ideas. Alternatively, after serving as an opening wedge into language, gesture could cease its role as a forerunner of linguistic change. We addressed this question in a sample of 40 typically developing children, each observed at 14, 18, and 22 months. The number of supplementary gesture-speech combinations the children produced increased significantly from 14 to 22 months. More importantly, the types of supplementary combinations the children produced changed over time and presaged changes in their speech. Children produced three distinct constructions across the two modalities several months before these same constructions appeared entirely within speech. Gesture thus continues to be at the cutting edge of early language development, providing stepping-stones to increasingly complex linguistic constructions.     

Prosodic disambiguation of syntactic structure: for the speaker or for the addressee?

Evidence has been mixed on whether speakers spontaneously and reliably produce prosodic cues that resolve syntactic ambiguities. And when speakers do produce such cues, it is unclear whether they do so "for" their addressees (the audience design hypothesis) or "for" themselves, as a by-product of planning and articulating utterances. Three experiments addressed these issues. In Experiments 1 and 3, speakers followed pictorial guides to spontaneously instruct addressees to move objects. Critical instructions (e.g., "Put the dog in the basket on the star") were syntactically ambiguous, and the referential situation supported either one or both interpretations. Speakers reliably produced disambiguating cues to syntactic ambiguity whether the situation was ambiguous or not. However, Experiment 2 suggested that most speakers were not yet aware of whether the situation was ambiguous by the time they began to speak, and so adapting to addressees' particular needs may not have been feasible in Experiment 1. Experiment 3 examined individual speakers' awareness of situational ambiguity and the extent to which they signaled structure, with or without addressees present. Speakers tended to produce prosodic cues to syntactic boundaries regardless of their addressees' needs in particular situations. Such cues did prove helpful to addressees, who correctly interpreted speakers' instructions virtually all the time. In fact, even when speakers produced syntactically ambiguous utterances in situations that supported both interpretations, eye-tracking data showed that 40% of the time addressees did not even consider the non-intended objects. We discuss the standards needed for a convincing test of the audience design hypothesis.