Sequence-Specific Impairment of Memory Formation by NCAM Antisense Oligonucleotides

The functional role of NCAM gene expression in memory formation was studied in the one-trial passive avoidance task in day-old chicks by pretraining injections of one of three different 18-mer end-protected oligonucleotides corresponding to positions 190-, 207-, and 332- of the NCAM Ig1 domain. Twenty-four-hour-old chicks were trained by pecking at a bitter-tasting bead and tested for avoidance 30 min, 3, 8, or 24 hr later. Memory retention was significantly reduced only in the group of animals injected with the NCAM antisense corresponding to position 207- (AS-ODN-207), and only if given twice, both immediately after hatching and 12 hr before training. This antisense was without effect on the general behavior of the chicks, training or acquisition, and did not produce observable neurotoxic damage. Under such conditions amnesia was evident by 3 hr after training and lasted until at least 24 hr after training. The two other tested oligonucleotides were without behavioral effect. To control for nonsequence-specific effects of AS-ODN-207, brains from injected and trained animals were processed for Western blotting and probed using anti-NCAM, anti-L1, and anti-actin antibodies. NCAM antisense corresponding to position 207- significantly reduced the level of NCAM, whereas the level of L1 and actin remained unchanged. These results confirm our earlier conclusion that NCAM is necessary for longer term memory retention.     

Frequency judgements: the problem of defining a perceptual event

In four experiments the conditions under which frequency judgments reflect the relative frequency of complex perceptual events were explored. Subjects viewed a series of 4 x 4 grids each containing seven items, which were letters and numbers in one of four typefaces. Later judgments of the relative frequency with which particular letters appeared in particular typefaces were unaffected by a warning about an upcoming frequency judgment task, but were affected by both the time available for processing the stimuli and the nature of the cover task subjects engaged in while viewing the grids. Frequency judgments were poor when exposure durations were less than 2 s and when the cover task directed subjects' attention merely to the locations of the items within the grids. Frequency judgments improved when the cover task directed subjects' attention to the identity of the stimuli, especially to the conjunction of letter and typeface. The results suggest that frequency estimation of complex stimuli may be possible only for stimuli that have been processed as phenomenal objects.     

Book reviews

Hall, G. (1991). Perceptual and associative leaning. Oxfod: Clarendon Press. Pp. xi + 300. ISBN 0-19-8524182-0. £35.00.

Rahmann, H., & Rahmann, M. (1992). The neurobilogical basis of memoy and behavior. New York: Springe Verlag. Pp. 292. ISBN 3-540-97545-4. £53.00.

Iversen, I.H., & Lattal, K.A. (Eds.). (1991). Experimental analysis of behavior, arts 1 and 2. Techniques in the behavioral and neural sciences, volume 6 (series editor: J.P Huston). Amstedam: Elsevier. Pp. 724. ISBN 0-444-8932-2. £50.00 (paperback).

Baudry, M., & Davis, J.L. (Eds.) (1991). Long-tem potentiation: A debate of current issues. Cambridge, Ma & London, England: MIT Press. Pp. xxii + 454. ISBN 0-262-02330-x. £62.95 (Hb).

The Biological Council Animal Reseach and Welfare Panel (1992). Guidelines on the handling and training of laboatory animals. Univer-sities Federation for Animal Welfare. Pp. ISBN 0-900767-77-4. £2.50.     

Drop the Small Talk When Establishing Baseline Behaviour in Interviews

The present experiment investigated the behavioural patterns of interviewees when comparing their baseline behaviour, prior to the interview, with their behaviour during the investigative interview. Similar to what has been advised in the police literature, the truthful baseline behaviour was established prior to the interview through non‐threatening questions. The investigative part of the interview then followed in which the interviewee was aware that they would be assessed on whether they were lying. During the investigative part, interviewees either discussed the job that they had (truth tellers, n = 128) or pretended to have (liars, n = 115). Findings revealed that both liars and truth tellers' behavioural patterns differed between the baseline behaviour and the investigative part of the interview. The findings suggest small talk should not be used as a baseline comparison with the investigative part of the interview when determining if the interviewee is being deceitful. An alternative way of using a baseline lie detection method, the comparable truth method, is discussed. Copyright © 2014 John Wiley & Sons, Ltd.     

Sensitive Topics,Missing Data,and Refusal in Social Network Studies: An Ethical Examination

We describe our ethics‐driven process of addressing missing data within a social network study about accountability for racism, classism, sexism, heterosexism, cis‐sexism, ableism, and other forms of oppression among social justice union organizers. During data collection, some would‐be participants did not return emails and others explicitly refused to engage in the research. All refusals came from women of color. We faced an ethical dilemma: Should we continue to seek participation from those who had not yet responded, with the hopes of recruiting more women of color from within the network so their perspectives would not be tokenized? Or, should we stop asking those who had been contacted multiple times, which would compromise the social network data and analysis? We delineate ways in which current discussions of the ethics of social network studies fell short, given our framework and our community psychology (CP ) values. We outline literature that was helpful in thinking through this challenge; we looked outside of CP to the decolonization literature on refusal. Lessons learned include listening for the possible meanings of refusals and considering the level of engagement and the labor required of participants when designing research studies.     

Career guidance in five English independent schools

English independent schools are not required to follow government statutory guidance in a number of aspects including career education and guidance, and yet many are actively engaged in careers work and this has caught the attention of policymakers. State schools are subject to statutory guidance but, according to Ofsted and other authorities, the majority fail to adequately support young people through their post-school transitions. Using evidence from five English independent schools, the type of provision they offer is analysed against benchmarks of good practice to identify where their support is focussed. The factors that shape the resourcing and design of career education and guidance in the five schools are also explored. The extent to which the practices observed in the case studies can be replicated in all, or a sub-set of state schools, is discussed in the light of policy influence of independent schools.     

Differential effects of arousal in positive and negative autobiographical memories

Autobiographical memories are characterised by a range of emotions and emotional reactions. Recent research has demonstrated that differences in emotional valence (positive vs. negative emotion) and arousal (the degree of emotional intensity) differentially influence the retrieved memory narrative. Although the mnemonic effects of valence and arousal have both been heavily studied, it is currently unclear whether the effects of emotional arousal are equivalent for positive and negative autobiographical events. In the current study, multilevel models were used to examine differential effects of emotional valence and arousal on the richness of autobiographical memory retrieval both between and within subjects. Thirty-four young adults were asked to retrieve personal autobiographical memories associated with popular musical cues and to rate the valence, arousal and richness of these events. The multilevel analyses identified independent influences of valence and intensity upon retrieval characteristics at the within- and between-subject levels. In addition, the within-subject interactions between valence and arousal highlighted differential effects of arousal for positive and negative memories. These findings have important implications for future studies of emotion and memory, highlighting the importance of considering both valence and arousal when examining the role emotion plays in the richness of memory representation.     

The role of self-esteem in the misinformation effect

Previous research using the Gudjonnson suggestibility scale has suggested a role for self-esteem in suggestibility, with participants low in self-esteem being more suggestible than participants high in self-esteem. Four experiments are presented examining the role of self-esteem in the misinformation effect and whether enhanced suggestibility effects in participants low in self-esteem reflect genuine memory impairment. In Experiments 1 and 4 participants completed a standard recognition test. In Experiment 2 participants completed the modified recognition test. In Experiment 3 participants completed a free recall test. In Experiments 1 and 4 participants low in self-esteem demonstrated greater misinformation effects than participants high in self-esteem. In Experiment 3 a 3-day retention interval was employed with the modified test and no differences were found between the two groups on the reporting of the new item. The findings suggest that participants low in self-esteem are particularly sensitive to demand characteristics and post-event suggestion but do not suffer from genuine memory impairment.