The effects of metabolic stress and vagotomy on emotional learning in an animal model of anxiety

The aim of the present study is to evaluate the role of the blood glucose (BG) level in emotional learning in the elevated plus maze (EPM), an animal model of anxiety. In Experiment 1, male Wistar rats were submitted to different EPM trial lengths (1- or 5-min). Blood samples were withdrawn before and after the maze exploration, through a polyethylene cannula chronically implanted into the jugular vein. In Experiment 2, the animals received either saline or 2-deoxy-D-glucose, a glucoprivic drug (2-DG, 250 or 500 mg kg(-1)) by i.p. route, 30 min before a 5-min EPM exposure and were retested in the maze (Trial1/Trial2 EPM procedure) 24 h later. In an independent group of rats, blood samples were withdrawn 0, 5, 15, and 30 min after 2-DG administration, through the jugular vein, to determine BG. In Experiment 3, the animals underwent a vagotomy and were tested in a Trial1/Trial2 EPM procedure four weeks later. The results showed that rats exploring the EPM for 5 min displayed increased fear and higher hyperglycemia than those exploring the EPM for 1 min. In addition, rats submitted to 5-min EPM Trial1 length displayed higher level of fear on Trial2, as well as higher percentage of shortening of the %Open arm entries and %Open arm time from Trial1 to Trial2, which characterizes the occurrence of emotional learning. In contrast, rats previously vagotomized or treated with 2-DG (500 mg kg(-1)) showed the same level of fear on both EPM trials and a low percentage of shortening, from Trial1 to Trial2, of the %Open arm entries and %Open arm time, indicating poor emotional learning. The data is discussed regarding the role of glycaemia in emotional learning in the EPM.     

Amnestic effect of intrahippocampal AM251, a CB1-selective blocker, in the inhibitory avoidance, but not in the open field habituation task, in rats

CB1 is the most abundant metabotropic receptor of the brain, being found in areas classically involved in learning and memory and present at higher density at presynaptic terminals. Different sets of evidence support the idea that endogenous ligands (endocannabinoids) to the CB1 receptors act as modulators of neurotransmission. In hippocampus, endocannabinoids seem to act as retrograde messengers mediating down-regulation of GABA release. Previous reports have described a cognitive impairment effect of cannabinoid agonists, or facilitation by antagonists. The scope of the present study is to investigate the effect of intrahippocampal administration of the CB1-selective antagonist, AM251, in two behavioral tasks. One hundred and twelve male Wistar rats with bilateral cannulae implanted in the CA1 region of the dorsal hippocampus were trained in a step-down inhibitory avoidance task (IA, footshock, 0.5 mA) or an open field habituation task (OF). Immediately, after training, animals received an infusion of 0.55, 5.5, and 55.5 ng/side of AM251 (Tocris), or its vehicle (DMSO/saline), via these cannulae. Our results show that AM251 disrupted memory consolidation of the IA task, but not the OF task, an effect that seems to be purely mnemonic since the drug showed no motor performance effects. Only the intermediate dose (5.5 ng/side) of AM251 was effective in IA and the absence of effect with the larger dose may be the consequence of non-specific binding. The fact that OF was not affected raises the possibility that this endogenous system requires some degree of aversiveness to be recruited. We propose that increased levels of endogenous cannabinoids in the hippocampus, following a training session, contribute to facilitate memory consolidation, a process that may have been disrupted with AM251.     

Who gets mad,sad, scared or happy at discipline? Emotion attributions explain child externalizing behaviour

Few studies have linked parental discipline with children's emotional experiences, and not much data explore children's emotional attributions to discipline linked to externalizing behaviour. With a sample from Brazil, this study examines which emotions children most aptly attribute to a protagonist facing spanking, time-out or inductive discipline for norm violations. We hypothesized that anger, sadness, and fear would have higher attribution rates at spanking or time-out, relative to inductive discipline and that happiness would have higher attribution rates at induction relative to the other discipline modalities. We expected these findings to be more pronounced in older children. Based on emotional functions, we also tested the role of neutrality and happiness attributions to discipline in children's externalizing behaviour. A two-way MANOVA, with discipline and child age as explanatory variables, showed that children attributed more anger at time-out or spanking than at induction, and more happiness and neutrality at induction than at either time-out or spanking. Older children attributed significantly more sadness and less fear or neutrality. Hierarchical regressions showed that child externalizing behaviour was negatively related to happy attributions in discipline independently of child emotion situation knowledge or demographics. The results are interpreted in light of a functional view of emotions.     

Lexical and semantic representations in the acquisition of L2 cognate and non-cognate words: evidence from two learning methods in children

How bilinguals represent words in two languages and which mechanisms are responsible for second language acquisition are important questions in the bilingual and vocabulary acquisition literature. This study aims to analyse the effect of two learning methods (picture- vs. word-based method) and two types of words (cognates and non-cognates) in early stages of children's L2 acquisition. Forty-eight native speakers of European Portuguese, all sixth graders (mean age = 10.87 years; SD= 0.85), participated in the study. None of them had prior knowledge of Basque (the L2 in this study). After a learning phase in which L2 words were learned either by a picture- or a word-based method, children were tested in a backward-word translation recognition task at two times (immediately vs. one week later). Results showed that the participants made more errors when rejecting semantically related than semantically unrelated words as correct translations (semantic interference effect). The magnitude of this effect was higher in the delayed test condition regardless of the learning method. Moreover, the overall performance of participants from the word-based method was better than the performance of participants from the picture-word method. Results were discussed concerning the most significant bilingual lexical processing models.     

Career Adapt-Abilities Scale–Portugal Form: Psychometric properties and relationships to employment status

The Career-Adaptabilities Scale (CAAS)–Portugal Form consists of four scales, each with seven items, which measure concern, control, curiosity, and confidence as psychosocial resources for managing occupational transitions, developmental tasks, and work traumas. Internal consistency estimates for the subscale and total scores ranged from good to excellent. The factor structure was fairly similar to the one computed for combined data from 13 countries. The CAAS–Portugal Form includes all the items in the International Form, and it adds one further national item to each scale. The syntheses of a variety of differential studies are presented to illustrate the importance of an adaptability measure to the field of career construction.     

Effective use of the blank comparison procedure in simple discrimination by infant capuchin monkeys: A methodological note

In studies of simple and conditional discrimination, procedures are needed to measure those aspects of stimuli that control behavior. The blank comparison procedure is one such procedure. It was designed explicitly for assessing S+ and S- functions when discriminative stimuli are presented simultaneously. In this procedure, a neutral stimulus serves sometimes as S+ and sometimes as S-. Its discriminative function is defined in relation to other stimuli in the display. The present study aimed to prepare 2 infant female capuchin monkeys for the effective use of the blank comparison procedure in a simple discrimination task. First, simple discrimination training was applied up to a stable accuracy criterion of ≥90%. This training was followed by the replacement of S+ and then of S- stimuli with new stimuli. Ultimately, trials with the blank comparison were introduced. Following this sequence, both monkeys immediately displayed highly accurate blank-comparison performances without the need for stimulus control shaping or other preparatory discrimination training. Thus, this procedure sequence may be an efficient, effective method for establishing blank-comparison baselines for experimental analyses of S+/S- discriminative functions and perhaps for other applications in teaching simple and conditional discrimination performances to this species and others.     

Effects of game location, period, and quality of opposition in elite handball performances

The aims of the present study were to identify (i) home advantage in elite handball according to the quality of opponent, (ii) the game periods where the teams score more goals, and (iii) the game statistics associated with the teams' success according to the game's location. The sample comprised 480 regular season games (2007-2009) from the Spanish Professional Handball League. The goals scored and shot effectiveness (6 m, 7 m, 9 m and fast breaks) were analyzed for each 5-min. game period in games between players of balanced and unbalanced quality. The home advantage was 64%, with higher values (71%) in balanced and lower values (55%) in unbalanced games. The 5-min. game periods in which teams scored more goals were the last 5-min. period of each half, especially in the second half. The effectiveness was only different in shots closer to the goal (6 m), which supports the territoriality theory of home advantage.     

Power and Legitimizing Ideologies in Hierarchy‐Enhancing vs. Hierarchy‐Attenuating Environments

According to social dominance theory, intergroup dynamics are strongly influenced by the social positions which individuals hold in society. Several studies suggest that holding a position of power can, sometimes automatically, generate negative attitudes and hostile behaviors toward subordinate social groups. However, at present, the studies carried out on these effects of power have not taken into account the influence of the normative context. Based on the distinction made by Sidanius and Pratto between “hierarchy‐enhancing” (HE) vs. “hierarchy‐attenuating” (HA) environments, this study examines if the effects of power on legitimizing cognitions vary as a function of the normative environment. A laboratory study and a study conducted in a natural environment confirm our hypothesis that individuals holding a powerful or dominant social role in a HE setting differ significantly from individuals holding an equally powerful role in a HA setting. The effects of power observed in past research probably resulted from studying power in hierarchy‐enhancing environments, but not in hierarchy‐attenuating ones.     

Angiotensin-(1-7)/Mas axis integrity is required for the expression of object recognition memory

It has been shown that the brain has its own intrinsic renin-angiotensin system (RAS) and angiotensin-(1-7) (Ang-(1-7)) is particularly interesting, because it appears to counterbalance most of the Ang II effects. Ang-(1-7) exerts its biological function through activation of the G-protein-coupled receptor Mas. Interestingly, hippocampus is one of the regions with higher expression of Mas. However, the role of Ang-(1-7)/Mas axis in hippocampus-dependent memories is still poorly understood. Here we demonstrated that Mas ablation, as well as the blockade of Mas in the CA1-hippocampus, impaired object recognition memory (ORM). We also demonstrated that the blockade of Ang II receptors AT1, but not AT2, recovers ORM impairment of Mas-deficient mice. Considering that high concentrations of Ang-(1-7) may activate AT1 receptors, nonspecifically, we evaluate the levels of Ang-(1-7) and its main precursors Ang I and Ang II in the hippocampus of Mas-deficient mice. The Ang I and Ang II levels are unaltered in the whole hipocampus of MasKo. However, Ang-(1-7) concentration is increased in the whole hippocampus of MasKo mice, as well as in the CA1 area. Taken together, our findings suggest that the functionality of the Ang-(1-7)/Mas axis is essential for normal ORM processing.