Some statistical treatments compatible with individual organism methodology

Consider experimental treatments with consequences so irreversible that baseline performance cannot be recovered. The conventional method of assessing the effects of such treatments by statistical means involves separate experimental and control groups. An alternative proposed here is to administer the experimental treatment to each subject, one subject at a time and in a random order; whenever any subject receives the experimental treatment, those subjects which have not yet received it receive a control treatment. This procedure permits results significant at the one-tailed 0.05 level to be obtained with four subjects; if a two-group procedure evaluated by means of the U test is used, a minimum of six subjects is needed for the same significance level. More generally, the procedure permits equal sensitivity to any experimental effect with over 30% fewer subjects than a two-group procedure. Extensions of the basic method are made to a variety of levels of the experimental treatment and to treatments without irreversible effects, and limitations of the method are discussed.     

Choice and delay of reinforcement

Pigeons were trained to peck either of two response keys for food reinforcement on equated aperiodic schedules. The distribution of responding at the two keys was studied as reinforcement was delayed for various durations. The relative frequency of responding at each key was shown to match the relative immediacy of reinforcement, immediacy defined as the reciprocal of the delay of reinforcement.     

Schedules using noxious stimuli. II: low intensity electric shock as a discriminative stimulus

The presence or absence of pulses of low intensity electric shock was used as a discriminative stimulus to control responding under fixed ratio reinforcement in the squirrel monkey. Initially brief periods of nonreinforcement were lengthened only when discriminative control was evident. Discriminative control was studied by (1) varying the duration of nonreinforcement periods; (2) reversing the stimulus conditions correlated with reinforcement and nonreinforcement periods; and (3) determining the minimum shock intensity necessary to maintain discriminative control. Stimulus control was not reliably affected by d-amphetamine, chlorpromazine, or morphine. The discriminative control by pulses of low intensity electric shock was similar to that by other discriminative stimuli, except that the control developed slowly and was better when the pulsing shock was correlated with reinforcement than when correlated with nonreinforcement.     

Drug effects in squirrel monkeys trained on a multiple schedule with a punishment contingency

The behavior of four monkeys trained on a multiple schedule was differentially sensitive to selected pharmacological agents. The three components of the multiple schedule were: (1) a variable-interval schedule in which responses were reinforced on the average of once per minute; (2) a concurrent schedule in which every tenth response was reinforced and every fifteenth response, on the average, was shocked; and, (3) a neutral stimulus in the presence of which responses were neither reinforced nor shocked. Pentobarbital, chlordiazepoxide, and meprobamate increased responding during each of the components. Scopolamine and d-amphetamine decreased variable-interval performance, had minimal effects on performance during the concurrent-schedule component, and increased responding in the presence of the neutral stimulus. Chlorpromazine decreased variable-interval responding and had slight effects on the responding during the other two components.