Time course and efficiency of protein synthesis inhibition following intracerebral and systemic anisomycin treatment

Since its discovery in the 1960s, anisomycin has been used for studying the impact of protein synthesis for manifold cerebral processes such as long-term plastic changes after learning. The common limitation of nearly all pharmacological experiments, including anisomycin treatment, is to precisely verify the affected brain regions. Here we illustrate anisomycin effects on protein synthesis in distinct brain regions of mice (C57BL/6JOlaHsd), revealing differences between three modes of anisomycin application (subcutaneous, s.c.; intraperitoneal, i.p.; local microinfusions into the hippocampus). Our method is based on inhibition of the incorporation of the radioactively-labelled amino acids [(35)S]-Methionine/Cysteine into newly synthesised proteins. Washing the brain slices before autoradiography removes pools of amino acids, which have not been incorporated into newly synthesised proteins, thus, illustrating pure protein synthesis. By comparing different routes of systemic anisomycin application (i.p. versus s.c.; 150 mg/kg) it became evident that the effect of i.p. injection of anisomycin is fully reversed after 6 h, whereas s.c. injection is inhibiting protein synthesis in the hippocampus even 9 h after application. Local microinfusions of anisomycin into the hippocampus were shown to have long-lasting effects as well, which reversed as late as 9 h after injection. The diffusion of anisomycin was maximal at 3 h after injection and more precisely confined to the intended area using a lower dose (20 microg/site) instead of the commonly used dose of 62.5 microg/site. The broad time window of anisomycin action, as revealed in our study, has to be considered, if it comes to the interpretation of time course studies within the context of protein synthesis-dependent processes.     

No Retrieval-Induced Forgetting Under Stress

ABSTRACT— Stress affects memory, yet no study has investigated the effects of stress on memory inhibition: Remembering not only facilitates later recall, but also inhibits retrieval of related material, a phenomenon known as retrieval-induced forgetting. We investigated the effects of stress on this mechanism, which is thought to adaptively guide memory selection. Participants learned categorized lists and were then exposed to either a psychosocial laboratory stressor or a cognitively challenging control treatment. They then actively retrieved parts of the previously learned material. Finally, memory for all initially learned items was tested. In the stress group, unlike in the control group, intervening retrieval practice did not impair final recall. Moreover, salivary cortisol levels increased and psychological well-being decreased in the stress group only. Thus, psychosocial stress abolishes retrieval-induced forgetting. This effect may result from stress-induced hormone release from the hypothalamic-pituitary-adrenal (HPA) axis and may have implications for educational, legal, and clinical issues.     

Do the Emotional Benefits of Optimism Vary Across Older Adulthood? A Life Span Perspective

This study examined whether the emotional benefits of dispositional optimism for managing stressful encounters decrease across older adulthood. Such an effect might emerge because age‐related declines in opportunities for overcoming stressors could reduce the effectiveness of optimism. This hypothesis was tested in a 6‐year longitudinal study of 171 community‐dwelling older adults (age range = 64–90 years). Hierarchical linear models showed that dispositional optimism protected relatively young participants from exhibiting elevations in depressive symptoms over time, but that these benefits became increasingly reduced among their older counterparts. Moreover, the findings showed that an age‐related association between optimism and depressive symptoms was observed particularly during periods of enhanced, as compared to reduced, stress. These results suggest that dispositional optimism protects emotional well‐being during the early phases of older adulthood, but that its effects are reduced in advanced old age.     

The interpersonal effects of emotions in negotiations: a motivated information processing approach

Three experiments tested a motivated information processing account of the interpersonal effects of anger and happiness in negotiations. In Experiment 1, participants received information about the opponent's emotion (anger, happiness, or none) in a computer-mediated negotiation. As predicted, they conceded more to an angry opponent than to a happy one (controls falling in between), but only when they had a low (rather than a high) need for cognitive closure. Experiment 2 similarly showed that participants were only affected by the other's emotion under low rather than high time pressure, because time pressure reduced their degree of information processing. Finally, Experiment 3 showed that negotiators were only influenced by their opponent's emotion if they had low (rather than high) power. These results support the motivated information processing model by showing that negotiators are only affected by their opponent's emotions if they are motivated to consider them.     

Social motives and strategic misrepresentation in social decision making

In 4 experiments, the authors studied the influence of social motives on deception and strategic misrepresentation. In a newly developed information provision game, individuals faced a decision maker whose decision would affect both own and other's outcomes. By withholding information or by giving (in)accurate information about payoffs, participants could try to influence other's decision making. Less accurate and more inaccurate information was given when the decision maker was competitive rather than cooperative (Experiment 1), especially when participants had a prosocial rather than selfish value orientation (Experiments 3 and 4). Accurate information was withheld because of fear of exploitation and greed, and inaccurate information was given because of greed (Experiment 2). Finally, participants engaged in strategic misrepresentation that may trick competitive others into damaging their own and increasing the participant's outcomes.     

Disturbed sense of identity in borderline personality disorder

Traditionally, personal identity is considered to be important for psychological health and adaptive functioning. Identity diffusion and other more severe forms of disturbance associated with personal identity are regarded as being essential parts of the borderline personality disorder. Moreover, disturbances in identity are seen as being part of the dynamic background for many of the symptoms and maladaptive behaviors found in borderline patients. It is argued, that the development of personal identity is intimately related to, and indeed dependent on, elements of modern culture, with significant cultural changes having affected the conditions under which human identity develops. Therefore, the identity diffusion seen in patients with borderline disorders must be understood in relation to not only the individual patient's personal history and inner structures but also contemporary late modern culture and social organization.     

The possessive self as a barrier to conflict resolution: effects of mere ownership, process accountability, and self-concept clarity on competitive cognitions and behavior

The authors propose that people have difficulty managing conflict because they quickly develop ownership of arguments and positions they use in the dispute, that these arguments and positions become part of their (extended) self-concept, and that any opposition or counterargumentation therefore becomes an ego-threat. Four studies reveal that individuals value arguments and beliefs more when these are associated with the self and that anticipated or real opposition triggers ego-defensive cognition and behavior, including competitive communication, retaliatory responses, negative perceptions of the partner, and attitude polarization. These effects were weaker when epistemic needs were raised through process accountability or when individuals had high rather than low self-concept clarity. The authors conclude that because people develop ownership of arguments and make these part of their self-concept, conflict is difficult to manage and bound to escalate.     

Perceived control of life regrets: good for young and bad for old adults

Age differences in the associations among intensity of regret, control attributions, and intrusive thoughts were investigated (N = 122, age range = 20-87 years). Given that the opportunities to overcome regrettable behavior decline with age, older adults' attributions of low internal control were expected to serve self-protective functions and facilitate deactivation of regret. In younger adults, by contrast, high levels of internal-control attributions might facilitate active change of regrettable behavior, resulting in low intensities of regret. The results showed that internal-control attributions were related to high intensity of regret and intrusive thoughts in older adults. Among younger adults, however, internal-control attributions were associated with low intensity of regret and low levels of intrusive thoughts.