Signaling Mechanisms Mediating BDNF Modulation of Synaptic Plasticity in the Hippocampus

Although recent studies indicate that brain-derived neurotrophic factor (BDNF) plays an important role in hippocampal synaptic plasticity, the underlying signaling mechanisms remain largely unknown. Here, we have characterized the signaling events that mediate the BDNF modulation of high-frequency synaptic transmission. Mitogen-associated protein kinase (MAPK), phosphotidylinositol-3 kinase (PI3K), and phospholipase C-γ (PLC-γ) are the three signaling pathways known to mediate neurotrophin signaling in other systems. In neonatal hippocampal slices, application of BDNF rapidly activated MAPK and PI3K but not PLC-γ. BDNF greatly attenuated synaptic fatigue at CA1 synapses induced by a train of high-frequency, tetanic stimulation (HFS). Inhibition of the MAPK and PI3K, but not PLC-γ, prevented the BDNF modulation of high-frequency synaptic transmission. Neurotrophin-3 (NT-3), a close relative of BDNF, did not activate MAPK or PI3K and had no effect on synaptic fatigue in the neonatal hippocampus. Neither forskolin, which activated MAPK but not PI3 kinase, nor ciliary neurotrophic factor (CNTF), which activated PI3K but not MAPK, affected HFS-induced synaptic fatigue. Treatment of the slices with forskolin together with CNTF still had no effect on synaptic fatigue. Thus, although the activation of MAPK and PI3K is required, the two together are not sufficient to mediate the BDNF effect. Inhibition of new protein synthesis by anisomycin or cycloheximide did not prevent the BDNF effect. These data suggest that BDNF modulation of high-frequency transmission is independent of protein synthesis but requires MAPK and PI3K and yet another signaling pathway to act together in the hippocampus.     

Assessing Hypochondriasis: Findings on the Survey of Health Concerns

We describe a brief self-report measure for assessing hypochondriacal fears in adults. Called the Survey of Health Concerns (SHC), the measure was developed using DSM IV criteria for diagnosing hypochondriacal disorder as a guide. The measure was standardized on a sample of 188 college students. It was shown to have good internal and temporal reliability over a 2-week period. Moreover, scores on the SHC correlated significantly with worry proneness as measured by the Penn State Worry Questionnaire, and with the Hs scale of the MMPI-2, which together support the convergent validity of the measure. While men and women scored the same, participants who were being treated for a medical condition scored higher than those who were not being treated. Men who were under a doctor's care scored especially high, although this was based on a small sample of men and the effect could be spurious. A principal component factor analysis revealed two primary factors: one that described participants who felt poorly, made frequent trips to the doctor, and were fearful of illness and dying, and another that described participants who were particularly fearful of infectious diseases and took active steps to avoid becoming sick. Relatively little is known about hypochondriacal disorder. One reason for the dearth of knowledge in this area is that there has been no practical and reliable way to assess hypochondriacal fears, or more generally speaking, people's tendency to worry about their health. The SHC may help to fill this void. Results are admittedly preliminary and further study in medical settings is needed.     

Developing conceptions of racism among young white adults in the context of cultural diversity coursework

Despite substantial research on racial attitudes and racial identity development among young white adults, little is known about the development of their conceptions about racism. The present study assessed a five-step, empirically based contextualist model of the development of conceptions about racism in young white adults, adapted from the research of Perry (1970), Kitchener and Fischer (1990), and others. A total of 55 young white adults participating in cultural diversity coursework responded to questionnaires about the nature and causes of racism and responses were classified according to the five-step model. Results indicated that many first-year white college students think about racism in dualistic terms, comparable to those described by Perry (1970) in some first-year students' epistemological concepts. Over a semester, many students showed transitions to higher steps, conceptually integrating more dimensions of the problem. Such developmental transitions may be highly context-specific. Implications for educational interventions are discussed.     

Creating embryos for research

ABSTRACT The 1987 Amendment to the Infertility (Medical Procedures) Act (Vic) allows the creation of embryos specifically for research purposes, as long as the proposed experiment takes place within 24 hours. The purpose of this paper is to determine whether there is aany significant ethical difference between creating embryos specifically for research and using those that are surplus from the new reproductive technologies. The relevant arguments in this debate can be grouped under three heads: those focussing on the embryo; those focussing on the possible consequences of these practices and those focussing on the women who donate the eggs.     

The impact of training in sexual counselling for drugs workers upon HIV related risk behaviours and attitudes among injecting drug users

Forty-five drug-using clients attending two drug services at a London hospital were interviewed before and after a training programme on sexual counselling delivered to staff working at the two units. Following staff training, clients reported increased interaction during counselling with staff on sexual issues. Results indicated that drug use and injecting behaviours were unaflected by the stag training and that changes in sexual behaviour were modest. Attitudinal changes were more substantial with more positive attitudes emerging to limiting the number of sexual partners and towards the use of condoms. The value of observed attitudinal changes and the importance of decision-making processes of clients for harm minimization are discussed     

The structure of the Hospital Anxiety and Depression Scale: Theoretical and methodological considerations

The Hospital Anxiety and Depression Scale (HADS; Zigmond - Snaith, 1983) is widely used; however, its factor structure is unclear, with studies reporting differing unidimensional, two-factor and three-factor models. We aimed to address some key theoretical and methodological issues contributing to inconsistencies in HADS structures across samples. We reviewed existing HADS models and compared their fit using confirmatory factor analysis (CFA). We also investigated methodological effects by comparing factor structures derived from Rasch and Principal Components Analysis (PCA) methods, as well as effects of a negative wording factor. An Australian community-dwelling sample consisting of 189 females and 158 males aged 17–86 (M = 35.73, SD = 17.41) completed the 14-item HADS. The Rasch Analysis, PCA and CFA all supported the original two-factor structure. Although some three-factor models had good fit, they had unacceptable reliability. In the CFA, a hierarchical bifactor model with a general distress factor and uncorrelated depression and anxiety subscales produced the best fit, but the general factor was not unidimensional. The addition of a negative wording factor improved model fit. These findings highlight the effects of differing methodologies in producing inconsistent HADS factor structures across studies. Further replication of model fit across samples and refinement of the HADS items is warranted.     

Psychological distress with relationship satisfaction is moderated by anticipatory relational savoring among non-deployed military partners

This study recruited participants whose partners were deployed for active-duty military service to examine whether anticipatory relational savoring moderated the association of psychological distress with relationship satisfaction. Two weeks prior to their partner's deployment (T1), participants (N = 73) completed a self-report measure of relationship satisfaction. Then, 2 weeks into their partner's deployment (T2), participants completed self-report measures of stress, loneliness, and depression (combined into a composite index of psychological distress), and relationship satisfaction. Participants also completed a stream-of-consciousness task at T2 in which they imagined and discussed their partner's return from deployment. We coded the stream-of-consciousness task for anticipatory relational savoring regarding their upcoming reunion with their deployed partner. We found that anticipatory relational savoring moderated the association of psychological distress with during-deployment relationship satisfaction after adjusting for demographics, interpersonal variables, and deployment-specific variables; the association did not hold after adjusting for pre-deployment relationship satisfaction, and thus was robust when considering the distress-satisfaction association during the deployment but was not when considering changes in relationship satisfaction from pre- to during-deployment. We discuss the potential importance of anticipatory relational savoring for this unique population.     

Experiencing Job Burnout: The Roles of Positive and Negative Traits and States

Extending recent research efforts on the effects of personality and moods at work, this study examined the impact of personality traits and mood states in job burnout. Specifically, the field study examined the role of 2 personality traits and positive and negative moods (states) in burnout among nurses working at 2 hospitals. Results indicate that extra-version significantly predicted the diminished accomplishment component of burnout, and neuroticism significantly predicted the exhaustion and depersonalization components. Thus, the findings indicate that personality dimensions predict burnout components differentially. Further, positive moods mediated the relationship between extraversion and accomplishment, while negative moods partially mediated between neuroticism and exhaustion. Thus, moods exhibited both direct and mediating effects. Implications for management and suggestions for future research are offered.     

Employing persons with severe disabilities: much work remains to be done

This article presents statistical data depicting the employment of persons with disabilities at a federal installation using a recruitment intervention designed to increase the presence of persons with disabilities. The data were obtained by reviewing archival recruitment accessions that span 5 years (1999–2003). The organization under study is a highly technical Department of Defense organization constituted primarily of engineers and scientists and has been in existence and a major contributor in national defense for many years. Earlier recruitment data are not available.