Spatial learning in rats is impaired by microinfusions of protein kinase C-gamma antisense oligodeoxynucleotide within the nucleus accumbens

The nucleus accumbens (NAcc) has been shown to play a role in motor and spatial learning. Protein kinase C (PKC) has been implicated in the mechanisms of initiation and maintenance of long-term potentiation that is thought to be involved in the storage of long-term memory. In the present study, the importance of de novo synthesis of PKC-gamma within the NAcc in the acquisition and retention of spatial discrimination learning was assessed using an antisense knockdown approach. Separate groups of Long-Evans rats were exposed to acute microinfusions (6microg/microl) of PKC-gamma antisense oligodeoxynucleotide (AS-ODN), control oligodeoxynucleotide (C-ODN) or vehicle into the NAcc at 24 and 3h before each training session. Behavioral findings showed that the blockade of NAcc-PKC-gamma translation caused impairments in the early phase of learning and retention of spatial information. Biochemical experiments showed that PKC-gamma expression was reduced and Ca(2+)/phospholipid-dependent protein kinase C (PKC) activity was blocked significantly in the AS-ODN-treated rats in comparison with control rats. The present findings suggest that NAcc-PKC-gamma plays a role during the early acquisition of spatial learning. Also, retention test results suggest that NAcc-PKC-gamma may be working as an intermediate factor involved in the onset of molecular mechanisms necessary for spatial memory consolidation within the NAcc.     

The language-specific nature of grammatical development: evidence from bilingual language learners

The fact that early lexical and grammatical acquisition are strongly correlated has been cited as evidence against the view that the language faculty is composed of dissociable and autonomous modules (Bates & Goodman, 1997). However, previous studies have not yet eliminated the possibility that lexical-grammar associations may be attributable to language-general individual differences (e.g. children who are good at learning words are good at learning grammar). Parent report assessments of toddlers who are simultaneously learning English and Spanish (n = 113) allow an examination of the specificity of lexical-grammar relationships while holding child factors constant. Within-language vocabulary-grammar associations were stronger than cross-language relationships, even after controlling for age, proportion of language exposure, general language skill and reporter bias. Similar patterns were found based on naturalistic language samples (n = 22), ruling out a methodological artifact. These results are consistent with the view that grammar learning is specifically tied to lexical progress in a given language and provide further support for strong lexical-grammatical continuity early in acquisition.     

Death anxiety and death obsession in Spanish students

This study examined the dimensional structure of Templer's Death Anxiety Scale and Abdel-Khalek's Death Obsession Scale. The responses of 289 Spanish students to the Spanish forms of both scales were evaluated by means of a principal components analysis with varimax rotation. Three significant factors were identified: Death Obsession, Cognitive-Affective, and Death Anxiety. The distribution of the factor loadings for the items of both scales on Factors 1 and 3 supported the discriminant validity of the constructs specific to each of the scales, while Factor 2 showed a common component in both scales characterized by cognitive and affective aspects in relation to the idea of death.     

Iberian rock lizards (Lacerta monticola) assess short-term changes in predation risk level when deciding refuge use

Prey might assess that risk level estimated in the first encounter with a predator was fixed in subsequent responses, whereas prey using flexible behavior would track short-term changes in risk. The authors examined, in the field, refuge use of Iberian rock lizards (Lacerta monticola) after simulated predator attacks and tested (a) how risk was assessed when multiple sources of risk were present simultaneously, (b) how short-term changes in risk level of successive attacks affected refuge use, and (c) whether the interval between attacks was important, insofar as attacks were considered to be coming from the same or different predators. Results suggest that lizards have a flexible estimate of risk that is based on multiple sources and flexibly dependent on previous estimates.     

Exploring the structure of Zuckerman's Sensation Seeking Scale, Form V in a Spanish sample

The psychometric properties of the original version of the Sensation Seeking Scale (Form V) were analyzed in a sample of 1,006 Spanish university students. The total sample was randomly split into two halves. Exploratory factor analyses were conducted on the calibration sample (n=504), whereas confirmatory factor analysis procedures were performed on the validation sample (n=502). As previous studies have suggested, exploratory factor structure indicated that several items load on a different factor or have loadings lower than .30. Those problems were surpassed when the best five items per factor were factor analyzed again. The confirmatory factor analyses showed that a 4-factor simple structure model gave a clearly imperfect fit. A model of 20 items (5 items per factor) gave a better fit, although with similar problems regarding the low reliabilities of the Experience Seeking and Boredom Susceptibility subscales. The need for improvement of the four-factor structure is discussed.     

On the representation of Portuguese gender-inflected words in the mental lexicon

This study aims at verifying whether Portuguese gender-inflected nouns and adjectives are represented as full forms as suggested by Spanish data (Dominguez, Cuetos, & Segui, 1999). A series of lexical decision experiments is reported. Grammatical gender, frequency dominance, and grammatical category are manipulated and cumulative frequency is controlled. The results do not provide support for a full form representation of gender-inflected words. They suggest that grammatical category, or the nature of the inflectional process involved (lexical or syntactic), affects the way words are represented and accessed. Shorter recognition latencies were obtained for nouns drawn from Feminine dominant gender-inflected pairs than from Masculine dominant pairs whereas a tendency in the opposite direction was observed in adjectives. The effect of frequency dominance appears, nevertheless, to be restricted to feminine nouns. The data are compatible with the view that masculine nouns and adjectives are represented as gender-unmarked forms. These results are discussed in relation to current dual-access models of word recognition and to the notion of "interpretability" of lexico-syntactic features, as put forward in the Minimalist Program of Generative Linguistics.     

Morphological family size in a morphologically rich language: the case of Finnish compared with Dutch and Hebrew

Finnish has a very productive morphology in which a stem can give rise to several thousand words. This study presents a visual lexical decision experiment addressing the processing consequences of the huge productivity of Finnish morphology. The authors observed that in Finnish words with larger morphological families elicited shorter response latencies. However, in contrast to Dutch and Hebrew, it is not the complete morphological family of a complex Finnish word that codetermines response latencies but only the subset of words directly derived from the complex word itself. Comparisons with parallel experiments using translation equivalents in Dutch and Hebrew showed substantial cross-language predictivity of family size between Finnish and Dutch but not between Finnish and Hebrew, reflecting the different ways in which the Hebrew and Finnish morphological systems contribute to the semantic organization of concepts in the mental lexicon.     

Touchscreen-enhanced visual learning in rats

The efficiency of traditional levers and of modern touchscreen technology for training rats on a computerized visual discrimination was studied in a series of observations. When compared with a lever-based discrimination procedure, the use of touchscreens supported the faster development of signal tracking behavior and acquisition of a two-stimulus simultaneous visual discrimination. It did not affect the final level of accuracy. Factors related to spatial proximity of the responses with the stimuli, sign-tracking, and increased ease of touchscreen motor responses were suggested as possible reasons for the touchscreen training advantage. This increased efficiency allows large numbers of animals to be tested quickly, a necessary requirement for studies involving genetic and physiological interventions.     

The on-line resolution of the sentence complement/relative clause ambiguity: evidence from Spanish

Two self-paced reading experiments investigated syntactic ambiguity resolution in Spanish. The experiments examined the way in which Spanish subjects initially interpret sentences that are temporarily ambiguous between a sentence complement and a relative clause interpretation. Experiment 1 examined whether the sentence complement preference found in English is observed in Spanish speaking subjects. In Experiment 2, verbal mood was manipulated in order to study the influence of verb-specific information on sentence processing. Since subcategorization for a subjunctive complement clause is generally assumed to be a lexical property of some verbs, the manipulation of the mood of the embedded verb affords us an interesting and novel way to examine the influence of lexical information on syntactic ambiguity resolution. Experiment 1 showed that Spanish speakers initially interpret the ambiguous that-clause as a sentence complement. Experiment 2 showed that verb-specific information, in particular, the information that specificies that a verb subcategorizes for a subjunctive complement, is accessed and used rapidly and affects the ambiguity resolution process. The results are discussed in relation to current models of sentence processing.