Stress-induced facilitation of host response to bacterial challenge in F344 rats is dependent on extracellular heat shock protein 72 and independent of alpha beta T cells

Activation of the in vivo stress response can facilitate antibacterial host defenses. One possible mechanism for this effect is stress-induced release of heat shock protein 72 (Hsp72) into the extracellular environment. Hsp72 is a ubiquitous cellular protein that is up-regulated in response to cellular stress, and modulates various aspects of immune function including macrophage inflammatory/bactericidal responses and T-cell function when found in the extracellular environment. The current study tested the hypothesis that in vivo extracellular Hsp72 (eHsp72) at the site of inflammation contributes to stress-induced restricted development of bacteria, and facilitated recovery from bacteria-induced inflammation, and that this effect is independent of alpha beta (αβ) T cells. Male F344 rats were exposed to either inescapable electrical tail-shocks or no stress, and subcutaneously injected with Escherichia coli (ATCC 15746). The role of eHsp72 was investigated by Hsp72-immunoneutralization at the inflammatory site. The potential contribution of T cells was examined by testing male athymic (rnu/rnu) nude rats lacking mature αβ T cells and heterozygous thymic intact control (rnu/+) rats. The results were that stressor exposure increased plasma concentrations of eHsp72 and facilitated recovery from bacterial inflammation. Immunoneutralization of eHsp72 at the inflammatory site attenuated this effect. Stressor exposure impacted bacterial inflammation and eHsp72 equally in both athymic and intact control rats. These results support the hypothesis that eHsp72 at the site of inflammation, and not αβ T cells, contributes to the effect of stressor exposure on subcutaneous bacterial inflammation.     

Two Sexes,Two Genders Only: Measuring Attitudes toward Transgender Individuals in Poland

Transphobia is an under-examined but important type of prejudice to study in Polish culture. Poland is a country where a majority of transgender people feel discriminated against. There is a need for a more evidence-based measures for researchers and practitioners to better understand transphobia. The main purpose of the present three studies (n?=?300 participants for each study) was to validate the Genderism and Transphobia Scale (GTS; Hill and Willoughby 2005) and the Transphobia Scale (TS; Nagoshi et al. 2008) in Polish culture and to identify the possible psychological and demographic factors that matter in forming attitudes toward transgender individuals. The results confirm that Polish versions of both the GTS and the TS are reliable instruments to measure attitudes toward transgender individuals. Moreover, the studies revealed that both traditional and modern homonegativity, right-wing authoritarianism, social dominance orientation, religious fundamentalism, attitudes toward gender roles, and biological and cultural beliefs about the origins of gender differences were significant predictors of transphobia. As in previous studies, men were more prejudiced toward gender nonconformists in comparison to women. These studies contribute well-adapted tools for measuring transphobia and data-driven collection of significant predictors of transphobia.

     

Facial reactions to emotional stimuli: Automatically controlled emotional responses

Based on a model in which the facial muscles can be both automatically/involuntarily controlled and voluntarily controlled by conscious processes, we explore whether spontaneously evoked facial reactions can be evaluated in terms of criteria for what characterises an automatic process. In three experiments subjects were instructed to not react with their facial muscles, or to react as quickly as possible by wrinkling the eyebrows (frowning) or elevating the cheeks (smiling) when exposed to pictures of negative or positive emotional stimuli, while EMG activity was measured from the corrugator supercilii and zygomatic major muscle regions. Consistent with the proposition that facial reactions are automatically controlled, the results showed that the corrugator muscle reaction was facilitated to negative stimuli and the zygomatic muscle reaction was facilitated to positive stimuli. The results further showed that, despite the fact that subjects were required to not react with their facial muscles at all, they could not avoid producing a facial reaction that corresponded to the negative and positive stimuli.     

The Emergence of Human Altruism:Preschool Children Develop a Norm for Empathy-based Comforting

ABSTRACT

The study examined whether preschool children conceive of empathy-based comforting as being an obligatory reaction toward others in emotional need. We presented 3- and 5-year-old children with three scenarios in which protagonists showed different reactions toward an agent who has hurt herself. One protagonist reacted antisocially by laughing at the agent, one ignored the agent, and one demonstrated empathy-based comforting. The 3-year-olds only protested against the antisocial protagonist. In contrast, the 5-year-olds protested against the protagonists who either acted antisocially or ignored the needy other while they selectively affirmed the protagonist who showed empathy-based comforting. The findings indicate that a norm for empathy-based comforting develops in the preschool years. Overall, our study demonstrates the emergence of a normative concern with the well-being of others, a central aspect of human altruism.