PKG-mediated MAPK signaling is necessary for long-term operant memory in Aplysia

Signaling pathways necessary for memory formation, such as the mitogen-activated protein kinase (MAPK) pathway, appear highly conserved across species and paradigms. Learning that food is inedible (LFI) represents a robust form of associative, operant learning that induces short- (STM) and long-term memory (LTM) in Aplysia. We investigated the role of MAPK signaling in LFI memory in vivo. Inhibition of MAPK activation in animals prior to training blocked STM and LTM. Discontinuing MAPK signaling immediately after training inhibited LTM with no impact on STM. Therefore, MAPK signaling appears necessary early in memory formation for STM and LTM, with prolonged MAPK activity required for LTM. We found that LFI training significantly increased phospho-MAPK levels in the buccal ganglia. Increased MAPK activation was apparent immediately after training with greater than basal levels persisting for 2 h. We examined the mechanisms underlying training-induced MAPK activation and found that PKG activity was necessary for the prolonged phase of MAPK activation, but not for the early MAPK phase required for STM. Furthermore, we found that neither the immediate nor the prolonged phase of MAPK activation was dependent upon nitric oxide (NO) signaling, although expression of memory was dependent on NO as previously reported. These studies emphasize the role of MAPK and PKG in negatively reinforced operant memory and demonstrate a role for PKG-dependent MAPK signaling in invertebrate associative memory.     

Do salespeople matter in competitive tenders?

Abstract

Organizational buyers are increasingly employing competitive tenders with objective buying criteria to mitigate the influence of personal relationships with suppliers and reduce the overall cost of buying. This paper investigates the role of salespeople’s relationships with buyers (i.e., purchasing managers) and how they affect supplier selection in such contexts. Drawing on data from 428 tenders across different buying organizations, this study shows that the quality of the salesperson’s relationship with the buyer influences the buyer’s evaluation of the tender proposal, which, in turn, affects supplier selection. Thus, the results support an indirect effect of salesperson relationship on supplier selection even in a tender context. In addition, the results indicate that the effect of a salesperson’s relationship on buyer’s proposal evaluation is contingent on the comprehensibility of suppliers’ proposals and buyer’s product knowledge. These results have significant theoretical and managerial implications for both buyers and suppliers in business-to-business (B2B) tender contexts.     

„Psychopathy“ im Kindes- und Jugendalter

The term psychopathy is defined as a severe personality pathology, which is characterized by extreme aggressive, antisocial and violent behavior as well as affective and interpersonal deficits. In recent years, research into the construct of psychopathy has often focused on the incidence and detection of such pathologies in children and adolescents. In this context, a point of interest is to explore the course and stability of psychopathic personality dimensions throughout youth. Results demonstrate clear evidence for the occurrence of personality traits characteristic for psychopathy in early childhood and adulthood, which facilitates early detection with the aid of age-specific diagnostic instruments.The distinct correlation between psychopathy and severe, repeated and largely treatment refractory violence, which results in substantial public damage, calls for further research in children and adolescents in order to define the construct of psychopathy more precisely, to further illuminate underlying pathologies and consequently develop more specific typologies and effective treatment strategies. To that end, it is of particular importance to correctly capture dimensional factors of psychopathy in youth and to examine their stability throughout stages of adolescent development.     

Patient Autonomy and Quality of Care in Telehealthcare

Telemedicine is a complex field including various applications and target groups. Especially telehealthcare is seen by many as a means to revolutionize medicine. It gives patients the opportunity to take charge of their own health by using self-tracking devices and allows health professionals to treat patients from a distance. To some, this means an empowerment of patient autonomy as well as an improvement in the quality of care. Others state the dangers of depersonalization of medicine and the pathologization of daily life. This paper examines the ethical implications of telehealthcare, focusing on patient autonomy and quality of care by analyzing metareviews, randomized controlled trials and narrative ethical analyses on the topic. As a result, we conclude that the technically enhanced encounter between patients and health professionals may mean an empowerment of patient autonomy when it goes along with a personal relationship based on trust, assistance and support. When it comes to the quality of care, telehealthcare may lead to an improvement as long it is adopted to the patient’s individual needs.     

Anticipatory Uncertainty: How Academic and Industry Researchers in the Life Sciences Experience and Manage the Uncertainties of the Research Process Differently

The institutional contexts of research increasingly require researchers to anticipate their productivity and the uncertainties inherent in their research. This applies to both academic researchers and to researchers in start-up companies. This creates a specific kind of uncertainty, anticipatory uncertainty, that we define as the state of being uncertain as to whether research processes will be productive in a specific time frame and along situated definitions of good performance. In the life sciences, this anticipatory uncertainty is experienced and managed differently, depending on how research is organized and the cultural resources available in specific institutional contexts. In biotechnology companies, there is a readiness to embrace dynamic changes in both research strategies and the organization of work in response to new developments in the progress of the overall research agenda. In academia, the ability of research groups to react with similar flexibility seems significantly constrained by the individual attribution of research work and credit, and the correspondingly high level of individual anticipatory uncertainty. This raises questions about how far the current organization of academic research allows epistemic uncertainty to be embraced and corresponding risks to be taken, rather than safe questions to be pursued.     

Task effects, performance levels, features, configurations, and holistic face processing: A reply to Rossion

A recent article in Acta Psychologica (“Picture-plane inversion leads to qualitative changes of face perception” by Rossion [Rossion, B. (2008). Picture-plane inversion leads to qualitative changes of face perception. Acta Psychologica (Amst), 128(2), 274-289]) criticized several aspects of an earlier paper of ours [Riesenhuber, M., Jarudi, I., Gilad, S., & Sinha, P. (2004). Face processing in humans is compatible with a simple shape-based model of vision. Proceedings of the Royal Society of London B (Supplements), 271, S448-S450]. We here address Rossion’s criticisms and correct some misunderstandings. To frame the discussion, we first review our previously presented computational model of face recognition in cortex [Jiang, X., Rosen, E., Zeffiro, T., Vanmeter, J., Blanz, V., & Riesenhuber, M. (2006). Evaluation of a shape-based model of human face discrimination using FMRI and behavioral techniques. Neuron, 50(1), 159-172] that provides a concrete biologically plausible computational substrate for holistic coding, namely a neural representation learned for upright faces, in the spirit of the original simple-to-complex hierarchical model of vision by Hubel and Wiesel. We show that Rossion’s and others’ data support the model, and that there is actually a convergence of views on the mechanisms underlying face recognition, in particular regarding holistic processing.     

Unattended exposure to components of speech sounds yields same benefits as explicit auditory training

Learning a second language as an adult is particularly effortful when new phonetic representations must be formed. Therefore the processes that allow learning of speech sounds are of great theoretical and practical interest. Here we examined whether perception of single formant transitions, that is, sound components critical in speech perception, can be enhanced through an implicit task-irrelevant learning procedure that has been shown to produce visual perceptual learning. The single-formant sounds were paired at subthreshold levels with the attended targets in an auditory identification task. Results showed that task-irrelevant learning occurred for the unattended stimuli. Surprisingly, the magnitude of this learning effect was similar to that following explicit training on auditory formant transition detection using discriminable stimuli in an adaptive procedure, whereas explicit training on the subthreshold stimuli produced no learning. These results suggest that in adults learning of speech parts can occur at least partially through implicit mechanisms.     

Social Freezing in Medical Practice. Experiences and Attitudes of Gynecologists in Germany

Surveys of the German public have revealed a high acceptance of social freezing, i.e. oocyte conservation without medical indication. Up to now, there are no investigations available on the experiences and attitudes of health professionals towards social freezing. Between August 2015 and January 2016, we surveyed gynecologists Germany-wide on the topic social freezing. Five gynecologists specialized in reproductive medicine and five office-based gynecologists in standard care were chosen for the survey. The survey was conducted with an explorative, qualitative research design. The demand for social freezing in Germany is low. With regard to their fertility age, most women attend consultations too late, they have only little previous knowledge and false expectations. The gynecologists consider it the duty of society and politics to provide for the compatibility of family and work. They relate late parenthood to disadvantages primarily for the children. A majority of the gynecologists interviewed tend to advise natural reproduction. Social freezing is often mistaken as a kind of fertility insurance. Thus, it is necessary that physicians inform women early about the possibilities and limitations of social freezing. In the first place, social freezing is not a medical or medical-ethical topic. Women consider the method as a possibility to ensure the compatibility of family and work. This compatibility should be mostly perceived as a political topic. It cannot be a medical task to solve this issue. In fact, a debate in society as a whole is necessary that includes all relevant actors.