A tether system for cardiovascular studies with the behaving micropig

A tether system has been developed for continuous monitoring of blood pressure in the micropig. The micropig is a suitable model for blood-pressure research because of the similarity of its cardiovascular system to that of humans and because of its sensitivity to high sodium intake. The system consists of a metal boom, attached via a universal joint to a wall 6 ft above floor level, that extends horizontally to the center of the enclosure. A fluid and electronic swivel affixed to the boom is connected to a flexible, hollow, metal tether that descends to a vest worn by the micropig. The vest contains a pressure transducer to which an indwelling arterial catheter is connected via a stopcock. The transducer cable and an infusion line ascend through the interior of the tether to the swivel. The system remains in equilibrium through a system of pulleys and counterweights. Continuous, 24-h recording shows a diurnal variation characterized by higher heart rate but lower blood pressure during the day than at night. The system has been found to be effective for continuous studies over intervals of a month or more.     

GRADER: A computer program that recommends student letter grades

GRADER, a computer program that makes student test and/or course letter grade recommendations to instructors, is described. The program uses an algorithm to combine the content mastery (percentage correct) and peer comparison (“curve”) approaches to set grade cutoffs. In addition to standardizing the procedures with which letter grade cutoffs are made, GRADER may help to reduce instructor grading subjectivity and obviate tendencies toward grade inflation or deflation.     

The effect of unilateral stellectomy on the regulation of heart rate during behavioral stress

Changes in heart rate were examined during classical aversive conditioning before and after either left (n=10) or right stellectomy (n=8). Heart rate (HR) significantly (p<0.01) increased in response to the conditional stimulus with a further increase noted during the unconditional stimulus. After right stellectomy (RSGx) the aversive stress elicited a significantly (p<0.01) smaller increase in heart rate (peak HR change: control 66.8 +/- 6.0; RSGx 36.1 +/- 6.8 beats/min). In contrast left stellectomy did not significantly alter the heart rate conditional response. Cardioselective beta-adrenergic receptor blockade, metoprolol HCl (BB, 1.0 mg/kg, n=8), significantly (p<0.01) reduced the heart-rate conditional response (peak HR change: control 66.8 +/- 6; BB 25.1 +/- 3.8 beats/min) to the same extent as noted during RSGx. These data suggest that the heart-rate increases elicited by aversive stress are mediated in part by sympathetic nerves that originate or pass through the right stellate ganglion. The residual HR increase noted after RSGx or BB probably results from the withdrawal of cardiac parasympathetic activity.     

Low-dimensional chaos maps learning in a model neuropil (olfactory bulb)

Quantification of a chaotic system can be made by calculating the correlation dimension (D2) of the data that the system generates (Packard et al., 1980). The D2 algorithm, however, requires stationarity of the generator, a feature that biological data rarely reflect (Mayer-Kress et al., 1988). So we developed the “point correlation dimension” (PD2), an algorithm that accurately tracks D2 in linked data of different dimensions (Carpeggiani et al., 1991). We now present a mathematical argument that, for stationary data, individual PD2s converge to D2 and we demonstrate that the algorithm rejects contributions made by bursts of noise. Data were obtained from the surface of the olfactory bulb of the conscious rabbit (64 electrodes, 640 Hz each, 1.3 sec epochs) before and after presentation of a novel or habituated odor. D2 could be calculated in only 1 of 10 novel-odor trials, whereas PD2 could be calculated in all. Both algorithms indicated that a novel odor evokes a spatially uniform dimensional increase. The PD2 uniquely exhibited the dimensional decreases that occur during inspiration and the gradients of mean dimension present during the nonstimulated control state. These control gradients remained unchanged without odor experience, but showed spatially specific PD2 increases following odor habituation. It is interpreted that, 1) the PD2 issensitive, accurate, and appropriate for dimensional assessment of biological data, 2) that during analysis of unfamiliar information a singleglobal process is transiently evoked in the neuropil, and 3) after experience multiplespatially specific processes tonically map the sites of learning. Grant Support: National Institutes of Health, HL 31164 and NS27745     

Neonatal imitation of tongue protrusion and mouth opening: Methodological aspects and evidence of early individual differences

The present work examines imitation of mouth opening and tongue protrusion in 32 full-term infants at three different occasions: When the infants are two to three days, three weeks, and three months old. The analysis focuses (1) on individual differences in imitative behaviour and (2) on how to operationalize the infants' responses. The overall group analysis revealed that imitation of tongue protrusion was statistically significant for both two- to three-day-old and three-week-old infants but not when the children had become three months old. No statistically significant effect was observed for imitation of mouth opening. Two different imitation indexes were constructed in order to assess individual differences in early imitative behaviour. Results show that short-term stability in imitative tendencies exists between the first and second observation. The results further reveal that methodological factors must be seriously considered when studying neonatal imitation: the overall imitation found for tongue protrusion is demonstrated to be dependent on how the infants' responses are coded.     

Sex stereotypes in Norway revisited: 1977–87

In 1977 sex-trait stereotypes in Norway were measured at the young adult level and at the level of five-year-olds. At the young adult level the Adjective Check List was used, and the results were analysed according to an affective meaning scoring system, and a scoring system based on Transactional Analysis. The instrument used for children was a picture-story technique (Sex Stereotype Measure II). Persistence and change in sex-trait stereotypes were examined by replicating the study in 1987. Quantitatively, young adults' sex-trait stereotypes seemed to have changed little during the last decade, but qualitative analyses indicated that stereotypes have changed in the direction of greater favourability, activity and strength towards women and in the reverse direction towards men. The children in the 1987 sample expressed less stereotype knowledge than children in the 1977 sample concerning traits which traditionally have been ascribed to men.     

Age-dependent facilitation of taste-footshock conditioning by prior exposure to the training context

Among adult rats, gustatory stimuli are easily associated with illness, but not with external unconditioned stimuli such as footshock. Recent evidence indicates, however, that this cue-to-consequence specificity may vary ontongenetically. The present studies examined the acquisition of an aversion to a taste paired with footshock in 5- and 15-day-old rats. Consistent with previous reports, 5-day-old rats avoided the taste that preceded footshock, while 15-day-old subjects did not express an aversion to the taste paired with footshock. Exposure to the training context for either 1 or 5 h prior to conditioning disrupted taste-footshock conditioning in the 5-day-old subjects. For the 15-day-old subjects, 1 h of pre-conditioning exposure to the training context had no effect on conditioning, whereas a longer duration of preexposure promoted conditioning to the taste cue. The results suggest ontogenetic differences in stimulus selection.     

Effects of anterior or dorsomedial thalamic ibotenic lesions on learning and memory in rats

Sprague-Dawley rats were used to study the effects of ibotenic acid lesions of the anterior (A.Th.) and the dorsomedial (MD) thalamic nuclei on learning and memory. Memory was assessed by employing a temporal alternation task in a straight alley with varying intertrial intervals. In addition, spatial orientation and response flexibility were evaluated on a radial maze and on a spatial reversal task (SSDR). The results indicated that MD rats required more trials to learn the temporal alternation task and exhibited impaired performance compared to A.Th. and control groups at the shortest delay (15 s). In contrast, compared to the control group, A.Th. subjects which required less trials to master the task and exhibited normal performance at the 15-s delay were impaired when the intertrial interval was increased to 45 s. Whatever the lesion, no impairments were found in the SSDR or the radial maze while only MD lesions were found to result in a night hyperactivity associated with greater food and water consumptions. These findings indicate that A.Th. and MD are differentially involved in learning and memory processes. It is suggested that the MD is mostly involved in registering new information while the A.Th. plays a role in the maintenance of information over time.     

Synaptic plasticity in the hypoglossal nucleus of female canaries: structural correlates of season, hemisphere, and testosterone treatment

The caudal portion of the hypoglossal nucleus (nXIIts) contains the motor neurons that control the syrinx in songbirds. In canaries, song occurs seasonally, is principally produced by males, and appears to be produced predominantly by muscles on the left side of the syrinx. The present study measures the effect of seasonal change and manipulation of testosterone levels on synapse number and morphology in nXIIts in adult female canaries. We find that synapse density is lower in testosterone-treated birds than in control birds and lower in fall than in spring. The number of vesicles per presynaptic profile increases in the spring as a result of a general increase in this measure in all synapses. The number of vesicles per presynaptic profile also increases with testosterone treatment, primarily due to an increase in the proportion of synapses associated with unusually high vesicle counts. Together, these changes suggest that large reserves of neurotransmitter may be necessary to sustain singing. Several ultrastructural differences between hemispheres are found. Postsynaptic thickenings are longer, and postsynaptic processes are larger on the left side than on the right side. In the spring, there are more vesicles per synapse on the left than on the right, but this lateralization is reversed in the fall. Thus, lateralization of song production is associated with lateral asymmetries in synapse morphology. These hemispheric differences are relatively small, like those seen at the light microscope level, encouraging further consideration of peripheral as well as CNS sources of functional lateralization. The seasonal and testosterone-induced changes in synapse number and morphology may be components of the periodic reorganization of canary vocalization.     

REACTANCE AND INTENTIONALITY ATTRIBUTIONS AS DETERMINANTS OF THE INTENT TO FILE A GRIEVANCE

Experimental studies were conducted in two unions to examine the effects of perceptual variables–namely, reactance and intentionality attributions–on the intent to seek redress from management action. Participants responded to a series of vignettes, each of which described a management action (formal punishment, informal warning, promotion denial) taken against an employee. The vignettes varied systematically in terms of the perceived threat to worker freedom posed by the action and the degree to which the action was motivated by a dispositional rather than an environmental attribution. Both studies demonstrated that greater threat and dispositional attributions provoked stronger intent to file a grievance. Implications of the findings were discussed for investigating and screening grievances.