Depolarization-initiated endogenous cannabinoid release and underlying retrograde neurotransmission in interneurons of amygdala

The depolarization is also important for the short-term synaptic plasticity, known as depolarization-induced suppression of excitation (DSE). The two major types of neurons and their synapses in the lateral nucleus of amygdala (LA) are prone to plasticity. However, DSE in interneurons has not been reported in amygdala in general and in LA in particular. Therefore, we conducted the patch-clamp experiments with LA interneurons. These neurons were identified by lack of adaptation in firing rate of action potentials. In this study, we show for the first time a transient suppression of neurotransmission at synapses both within the local network and between cortical inputs and interneurons of the LA. The retrograde neurotransmission from GABAergic interneurons were comparable with that of glutamatergic pyramidal cells. That is the axonal terminals of cortical inputs do not posses selectivity toward two neuronal subtypes. However, the DSE of both types of neurons involve an increase in intracellular Ca²⁺ and the release of endogenous cannabinoids (eCB) and activation of presynaptic CB1 receptors. The magnitude of DSE was significantly higher in interneurons compared with pyramidal cells, though developed with some latency.

…I made experiments on myself and my assistant, using smaller doses, and not repeating them so often… — Clouston 1870

The biological actions of endogenous cannabinoids (eCB) occur by binding to the CB₁ and CB₂ receptors throughout the whole body (Ameri 1999; Pertwee 2006; Hill et al. 2007; Yoshida et al. 2011). The density of CB₁ receptors in the amygdala is comparably high in mammals (Herkenham et al. 1990).Amygdala similar to hippocampus is important for memory formation and often studied to elucidate plasticity at cellular level using the classical paradigm of Pavlov that continuously serves as a substrate (Pavlov 1927; Bliss and Lomo 1973; Rogan et al. 1997). The amygdala not only receives, but also sends behavior underlying signals into other regions (Racine et al. 1983; Aggleton and Mishkin 1984). While the role of hippocampus is crucial for memory formation, those associated with many different kinds of emotions are mainly modulated by the amygdala (Bucherelli et al. 2006; Fujii et al. 2020). The memory enabling substrate is a long-term potentiation (LTP) of neurotransmission into the postsynaptic neurons (Rogan et al. 1997; Kodirov et al. 2006).The short-term synaptic plasticity in the form of depolarization-induced suppression of either excitation or inhibition (DSE and DSI) has been reported in several regions of the brain (Alger et al. 1996; Kano et al. 2009; Ivanova and Storozhuk 2011). We have discovered DSE in the lateral amygdala (LA), specifically at cortical inputs into the pyramidal neurons (Kodirov et al. 2010).Despite the extensive studies on DSE and DSI, there are only three papers on interneurons that we are aware of. Two of them describe the presence of these phenomena: One study was carried out on parvalbumin immunoreactive interneurons of the stratum radiatum in the hippocampus (Ali 2007) and another on cerebellar stellate and basket cells (Beierlein and Regehr 2006). However, none of the cortical interneurons exhibited DSI despite the presence of a functional and cannabinoid-sensitive inhibitory inputs (Lemtiri-Chlieh and Levine 2007). The retrograde neurotransmission (Llano et al. 1991) takes place via the release of two natural ligands of endogenous cannabinoids anandamide and 2-arachidonoyl-glycerol (Urbanski et al. 2009). These ligands also suppress the evoked excitatory neurotransmission when applied exogenously in vitro (Ameri et al. 1999; Ameri and Simmet 2000; Lemak et al. 2007).Since DSE in interneurons has not been reported in amygdala and we demonstrated the existence of DSE in pyramidal cells of LA (Kodirov et al. 2010), we then studied the same phenomenon in regard to interneurons; the main question was whether or not does depolarization-induced mobilization of eCBs from the two types of postsynaptic LA neurons cause similar retrograde modulation of cortical inputs? Subsequently, DSE between the presynaptic terminals and interneurons was shown, and we found that its properties are similar to those in pyramidal cells of LA. Our study documents the participation of endogenous cannabinoids of interneurons in DSE.     

Einprägen und Aktivierung bei gleichzeitiger Variation der Absichtlichkeit des Lernens und der Ich-Bezogenheit des Lernstoffs

Zusammenfassung Eine Gruppe von Wörtern mit höherer Ich-Bezogenheit und eine von Wörtern mit niedrigerer Ich-Bezogenheit war einmal absichtlich, einmal beiläufig einzuprägen. Als abhängige Variablen dienten die Zahl der behaltenen Glieder und — zur Bestimmung des Aktiviertheitsgrades während des Einprägens — die Höhe des elektrischen Hautwiderstands. Sowohl einzeln als auch in Wechselwirkung verursachten die genannten unabhängigen Variablen bedeutsame Unterschiede hinsichtlich des Behaltens und der Aktivisierung. Der Deutung der Ergebnisse wurde die Annahme zugrunde gelegt, daß Aktivierungsvorgänge zu den Geschehnissen gehören, die für das Behalten von Bedeutung sind.

---

Summary Two series of nouns with high and low emotional tone had to be memorized both intentionally and incidentally. During learning the amount of electrodermal skin resistance was registered as a measure of organismic activation, thereafter the Ss had to recall the words presented previously. The independent variables mentioned led to significant main and interaction effects in regard to both retention and activation data. The interpretation of the results is based on the assumption that retention is affected by activational processes.

     

Drivers and outcomes of salespersons’ value opportunity recognition competence in solution selling

Abstract

In B2B markets, firms seek to provide customer solutions instead of merely selling goods or services. As boundary-spanners, salespeople are pivotal for implementing this strategic shift. Yet, extant literature provides limited insights into salesperson’s resources and competencies required for customer solutions, particularly in the early phases of solution selling. This research focuses on salesperson’s value opportunity recognition competence (VOR), which is a central requirement for salespeople to be able to navigate the early phases of solution selling. Analyzing large-scale, multi-level data of 799 salespeople and their respective sales managers in 29 sales organizations, the authors investigate the role of different salesperson resources and work environment characteristics for strengthening their VOR. The authors find that salespeople need both customer and technical knowledge, but customer knowledge is more important. Salespeople also can substitute individual technical knowledge with strong internal relations, but strong customer relations are no substitute for individual knowledge about customers’ business models and processes. Formalization turned out to be a double-edged sword in the context of VOR development, while transformational leadership has positive effects only. The findings bear concrete implications for improving the selection, training, and work environment of solution salespeople.     

Do Co-Worker Conflicts Enhance Daily Worries about Job Insecurity: A Diary Study

The long-term negative consequences of job insecurity on employees’ health and well-being have been demonstrated by several studies, but there is very little evidence on the daily experience of job insecurity and on the factors that may influence it. Therefore, we investigated whether short-term changes occur in the experience of job insecurity and whether these are influenced by daily co-worker conflicts. We carried out a diary study, in which 66 employees answered a questionnaire over the course of five working days. We conducted a multilevel analysis in which we included co-worker conflicts as a predictor, and type of contract, emotional stability, and aggregated job insecurity perceptions as control variables. Our results revealed that job insecurity varies on a daily level, and that 23 per cent of the variance could be explained at a within-person level. Co-worker conflicts were a significant positive predictor for perceived job insecurity in subsequent days after controlling for aggregated job insecurity perceptions at person level. Reversed causation was not found. Practical implications for organisations should focus on the promotion of positive social relations in the work environment in order to mitigate or avoid the negative consequences of social stressors in uncertain times.     

Stimulus and response anticipation in a serial reaction task

When subjects are confronted with sequences of stimuli that are constructed according to certain rules, they tend to improve their performance in terms of lower error rates and/or faster reaction times as compared to random sequences of the same stimuli, even if they are not able to report the regularities of events.