Automated assessment of conditioning parameters for context and cued fear in mice

A behavioral technique often used to evaluate the cognitive performance of rats and mice is the fear conditioning paradigm. During conditioned fear experiments, freezing responses shown by rodents after exposure to environmental stimuli previously paired to an aversive experience provide a behavioral index of the animal's associative abilities. The present study examined the ability of a computer-controlled automated Freeze Monitor system for recording immobility behavior in mice. The sensitivity of the automated procedure to detect group differences caused by the application of various training protocols was also evaluated. Statistical analyses revealed significant positive correlations between immobility scores obtained with the automated apparatus and hand-scored data collected by a continuous or a time-sampling method. Behavioral patterns recorded by the computerized system were very similar to those obtained by the hand-scoring methods adopted. In particular, during context testing, exposure to environmental stimuli previously paired with a mild foot shock (unconditioned stimulus [US]) evoked increased immobility behavior in mice conditioned with the US compared with levels of immobility displayed by mice previously confined to the same contextual stimuli without receiving the US. Moreover, although during conditioned stimulus (CS) testing, mice previously exposed to the US displayed high levels of immobility when confined to environmental cues much different from those paired with the US (contextual fear generalization), both hand-scored and automated results revealed the effect of CS–US pairing (increased immobility) only in mice trained to associate the two stimuli (paired group) but not in mice exposed to both CS and US separated by a 40-sec time interval (unpaired group) or in mice receiving only the US (US group) during conditioning sessions. Overall, the results show associative conditioning measured in an automated apparatus and highlight the utility of obtaining both latency as well as beam interruption parameters.     

Family Economic Well-Being,and (Class) Relative Wealth: An Empirical Analysis of Life Satisfaction of Secondary School Students in Three Italian Cities

The aim of the study is to shed light on an under-investigated topic—the determinants of life satisfaction of the young—by means of an econometric analysis which focuses specifically on the relationship between household wealth and life satisfaction of secondary school students living in three Italian cities: Rome, Milan and Genoa. We find that family home ownership, mortgages and (class) relative wealth significantly affect the life satisfaction of students. Other significant controls are geographical residence (those living in Milan are significantly less satisfied with life), the mother’s occupation, and trust in family and friendships. The characteristics of our household wealth variables make it hard to conceive any inverse causality (and endogeneity highly unlikely), thus suggesting a direct causality nexus for these factors. We explain why our research provides important insights that should be taken into account when developing policies to promote the subjective well-being of the young.     

Biases of attention in chronic smokers: Men and women are not alike

The activation of motivational systems by stimuli in the environment that are associated with rewarding experiences is able to trigger plastic changes in the brain, thereby altering the attentional priority of those stimuli. As a result, attentional deployment is often abnormal in addiction, with drug-related stimuli attracting attention automatically and gaining control over behavior. For example, smokers show attentional biases toward smoke-related cues, but the mechanisms underlying these effects and the nature of their link to addiction are still debated. Here, we investigated the influence of gender and individual factors on the temporal dynamics of attentional deployment toward smoke-related stimuli in young smokers. Crucially, we found a striking gender difference, with only males exhibiting a typical attentional bias for smoke-related items, and the bias revealed strong time dependency. Additionally, for both males and females, various personality traits and smoking habits predicted the direction and strength of the measured bias. Overall, these results unveil a crucial influence of several predictors—notably, gender—on the biases of attention toward smoke-related items in chronic smokers.     

(PSEUDO)SCIENCE,RELIGIOUS BELIEFS,AND HISTORIOGRAPHY: ASSESSING THE SCIENTIFICATION OF RELIGION'S METHOD AND THEORY

In the recent past, attempts to revitalize historico‐religious studies have challenged the charismatic appeal of some of the most celebrated scholars of the twentieth century. At the same time, the old and ideological frameworks that characterized the field have been critically analyzed and deconstructed. The disciplinary status quo, taken for granted for quite a long time, has been shaken to its foundation, paving the way for new approaches. However, the postmodern tenet of problematizing any authority has also become a convenient shortcut to blur the distinction between scientific signal (i.e., knowledge systematically obtained via rational inquiry) and nonepistemic noise (i.e., pseudoscience). Despite this troublesome feature, some scholars have deployed postmodern and poststructuralist tools to study the genealogy, reception, implementation, and diffusion of cultural representations within the aforementioned academic discipline. The present article briefly reviews one of the most recent and remarkable examples of such scholarship, that is, The Scientification of Religion: An Historical Study of Discursive Change, 1800–2000 (von Stuckrad 2014).     

Is defining life pointless? Operational definitions at the frontiers of biology

Despite numerous and increasing attempts to define what life is, there is no consensus on necessary and sufficient conditions for life. Accordingly, some scholars have questioned the value of definitions of life and encouraged scientists and philosophers alike to discard the project. As an alternative to this pessimistic conclusion, we argue that critically rethinking the nature and uses of definitions can provide new insights into the epistemic roles of definitions of life for different research practices. This paper examines the possible contributions of definitions of life in scientific domains where such definitions are used most (e.g., Synthetic Biology, Origins of Life, Alife, and Astrobiology). Rather than as classificatory tools for demarcation of natural kinds, we highlight the pragmatic utility of what we call operational definitions that serve as theoretical and epistemic tools in scientific practice. In particular, we examine contexts where definitions integrate criteria for life into theoretical models that involve or enable observable operations. We show how these definitions of life play important roles in influencing research agendas and evaluating results, and we argue that to discard the project of defining life is neither sufficiently motivated, nor possible without dismissing important theoretical and practical research.     

A Case of Partial Biotinidase Deficiency Associated With Autism

We report the case of a child with partial biotinidase deficiency and autistic developmental disorder. We arrived at the diagnosis of biotinidase deficiency when the child was almost 4 years of age. Consequently, he began cofactor biotin treatment (10 mg daily) which did not resolve his autistic behavior. His younger brother was affected by partial biotinidase deficiency diagnosed at birth through our neonatal screening program. He was precociously treated with cofactor biotin therapy (10 mg daily) and did not show any behavioral abnormality or developmental delay. Since the brain is quite vulnerable to biotin deficiency, delayed biotin therapy could result in neurological damage. Our patient is the first case of partial biotinidase deficiency associated with autism. We hypothesize that the low biotinidase activity could have caused biotin deficiency in his brain and cerebrospinal fluids and consequently serious neurological problems, such as stereotyped and autistic behaviors, which were irreversible in spite of biotin supplementation.     

Exploring the validity of scores from the Rosenberg Self-Esteem Scale (RSES) in Burundi: A multi-strategy approach

The present study aimed to validate the Rosenberg Self-Esteem Scale (RSES) in Burundi, through a multi-strategy approach used in cross-cultural studies. Respondents were 906 health workers (men = 56%; caregivers 60%). They responded to a bilingual version of RSES. We utilised Confirmatory Factorial Analysis (CFA) with structural equation modelling and a back translation test to explore the structure of the RSES and the reliability of scores from the scale. Data from an independent sample were analysed for the reliability of scores assessment. CFA results suggested that the global RSES factor was likely contaminated by a method-effect; mainly associated with negatively worded items. Internal consistencies and a back-translation test demonstrated that the negatively worded items were unsuitable in this context. The independent sample study confirmed poor reliability and internal consistency of scores for both alternative language versions of the RSES. Our data suggested that an overall cultural effect, rather than a merely specific language effect, may undermine the cross-cultural transportability of the Western scale.     

Substitution effects in a generalized token economy with pigeons

Pigeons made repeated choices between earning and exchanging reinforcer‐specific tokens (green tokens exchangeable for food, red tokens exchangeable for water) and reinforcer‐general tokens (white tokens exchangeable for food or water) in a closed token economy. Food and green food tokens could be earned on one panel; water and red water tokens could be earned on a second panel; white generalized tokens could be earned on either panel. Responses on one key produced tokens according to a fixed‐ratio schedule, whereas responses on a second key produced exchange periods, during which all previously earned tokens could be exchanged for the appropriate commodity. Most conditions were conducted in a closed economy, and pigeons distributed their token allocation in ways that permitted food and water consumption. When the price of all tokens was equal and low, most pigeons preferred the generalized tokens. When token‐production prices were manipulated, pigeons reduced production of the tokens that increased in price while increasing production of the generalized tokens that remained at a fixed price. The latter is consistent with a substitution effect: Generalized tokens increased and were exchanged for the more expensive reinforcer. When food and water were made freely available outside the session, token production and exchange was sharply reduced but was not eliminated, even in conditions when it no longer produced tokens. The results join with other recent data in showing sustained generalized functions of token reinforcers, and demonstrate the utility of token‐economic methods for assessing demand for and substitution among multiple commodities in a laboratory context.