Hippocampal PKA/CREB pathway is involved in the improvement of memory induced by spermidine in rats

Spermidine (SPD) is an endogenous polyamine that modulates N-methyl-D-aspartate (NMDA) receptor function, and has been reported to facilitate memory formation. In the current study we determined whether or not the PKA/CREB signaling pathway is involved in SPD-induced facilitation of memory of inhibitory avoidance task in adult rats. The post-training administration of the cAMP-dependent protein kinase (PKA) inhibitor, N-[2-bromocinnamylamino)ethyl]-5-isoquinoline sulfonamide [H-89, 0.5 ρmol intrahippocampal (ih)] or the antagonist of the NMDA receptor polyamine-binding site (arcaine, 0.02 nmol ih) with SPD (0.2 nmol ih) prevented memory improvement induced by SPD. Intrahippocampal administration of SPD (0.2 nmol) facilitated PKA and cAMP response element-binding protein (CREB) phosphorylation in the hippocampus 180 min, but not 30 min, after administration, and increased translocation of the catalytic subunit of PKA into the nucleus. Arcaine (0.02 nmol) and H-89 (0.5 ρmol) prevented the stimulatory effect of SPD on PKA and CREB phosphorylation. These results suggest that memory enhancement induced by the ih administration of SPD involves the PKA/CREB pathways in rats.     

Latin-American immigrant women and mental health: differences according to their rural or urban origin

Spain is one of the EU countries that receive most immigrants, especially from Latin America. The process of migration implies a high level of stress what may have repercussions for the mental health of immigrants. The purposes of this study were: (a) to determine whether the degree of mental health of immigrant women differs according to their rural or urban origin, (b) to compare the mental health of immigrant women with that of the female normative sample of host population (Spain). A sample of 186 Latin American immigrant women (142 from urban areas and 44 from rural areas) was recruited in Barcelona by means of a consecutive case method. A structured interview and the SCL-90-R were administered. The results indicated that the immigrant women from rural origin reported higher levels of psychological symptomatology than those from urban areas. Immigrants reported higher levels of psychological symptomatology than the native female population and in most of the psychological symptoms exceeded 90% of the native Spanish population. Migration is a powerful stressor which may lead to psychological distress. Being female of rural origin and being in an illegal situation is related with an increase in symptomatology.     

Quality of Life in Old Age: Problematic Issues

Quality of Life (QoL) is a concept widely used in gerontology, as well as in other fields such as anthropology, health sciences, psychology, sociology and political sciences. Historically, although Aristotle is considered as an antecedent, the term QoL emerged in about the sixties in scientific literature. In the field of ageing, it is considered as an outcome of projects, programmes, services or policies and is used for describing populations, contexts and individuals. QoL is considered by most experts as a multidimensional concept involving multiple domains (health, psychological, social and environmental), containing objective and subjective components. Nevertheless, in recent years, QoL has been reduced to the subjective appraisal, to health or to subjective psychological attributes such as well-being, happiness or life satisfaction. Moreover, conceptual confusions can be found between QoL and other concepts related to positive ageing. In this conceptually-driven paper, after reviewing a set of expert and lay conceptualizations of QoL and identifying the diversity of its components, three critical issues will be discussed: its reduction to health or to the subjective appraisal of a set of domains, the confusion of QoL with other subjective or positive concepts and, finally, its methodological reductionism to self-reports as an exclusive procedure for QoL data collection. From these criticisms some conceptual and methodological suggestions are proposed.     

Technology, Methodology and Intervention: Performing Nanoethics in Portugal

During the last few decades we have witnessed a proliferation of exercises dealing with the public participation of citizens in various different dimensions of their societies, including issues of science and technology. On the one hand, these mechanisms provide more robust forms of public engagement with matters that were traditionally dealt with by experts; on the other hand, they raise concerns relating to their design, efficiency or potential for the empowerment of citizens. As part of the EC-funded project DEEPEN (Deepening Ethical Engagement and Participation in Emerging Nanotechnologies) a research team in Coimbra, Portugal, was put in charge of identifying the ethical and social “impacts” of emerging nanotechnologies, transforming the traditional focus groups through the incorporation of two methodological innovations: the Pedagogy of the Oppressed and the Theatre of the Oppressed. This article reflects on the outcomes and complexities of the introduction of these two methodologies. Since the participants had little or no information on nanotechnologies, we reflect on the politics of these focus groups by exploring how issues of intervention, subjectivity, representation and agency were interconnected during this exercise of public participation in Science and Technology, analyzing the role of social sciences in developing nanoethics.     

Relationship between dispositional optimism/pessimism and stress coping strategies

Recent studies confirm that dispositional pessimism is related with a worse state of physical health, in comparison with dispositional optimism . The efficacy of stress coping strategies has been considered as a mediating mechanism in this relationship. The aim of the present study is to analyze in a sample of university students (n = 200) the association between dispositional optimism/pessimism and different categories of self-reported somatic symptoms , and evaluate the possible mediation of the stress coping strategies in this relationship. The results indicate that: 1) pessimism is positively related with the report of physical symptoms, whereas optimism is negatively associated to somatic complaints; and 2) this relationship can be mainly explained by the differential utilization of the coping strategy denominated self-criticism (characteristic of pessimism).     

Why do people change in therapy? A preliminary study

Why do people change in therapy? A preliminary study. This work represents the beginning of a new way to study the processes that can explain the therapeutic change. The method used is the observation and analysis of therapeutic sessions directly recorded and developed inside a behavioural approach. The category system tries to carry out a functional analysis of the therapist-client interaction which enables the identification of learning processes that work in clinical setting and explain success in therapy. Outcomes are provisional but show the utility of this initial trend of investigation to the comprehension of clinical phenomenon. Conclusions also emphasize the importance of associative learning processes on the therapeutic change.     

Conditioned and unconditioned fear organized in the periaqueductal gray are differentially sensitive to injections of muscimol into amygdaloid nuclei

The lateral and basolateral nuclei of the amygdala (LaA and BLA, respectively) serve as a filter for unconditioned and conditioned aversive information that ascends to higher structures from the brainstem, whereas the central nucleus of the amygdala (CeA) is considered to be the main output for the defense reaction. It has been shown that the dorsal periaqueductal gray (dPAG) is activated by threatening stimuli and has important functional links with the amygdala through two-way anatomical connections. In this work, we examined the influence of chemical inactivation of these nuclei of amygdala on the freezing and escape responses induced by electrical stimulation through electrodes implanted in the dPAG of Wistar rats. Each rat also bore a cannula implanted in the LaA, BLA or CeA for injections of muscimol (0.5 microg/0.5 microL) or its vehicle. The duration of freezing behavior that outlasts electrical stimulation of the dPAG was also measured. On the following day, these animals were submitted to a contextual fear-conditioning using foot shocks as unconditioned stimulus. Conditioned freezing to contextual cues previously associated with foot shocks was also inhibited by injections of muscimol into these amygdaloid nuclei. The contextual conditioned freezing behavior is generated in the neural circuits of conditioned fear in the amygdala. The data obtained also show that injections of muscimol into the three amygdaloid nuclei did not change the aversive threshold of freezing, but disrupted the dPAG post-stimulation freezing. Previous findings that the latter freezing results directly from dPAG stimulation and that it is not sensitive to a context shift suggest that it is unconditioned in nature. Thus, the amygdala can affect some, but not all, aspects of unconditioned freezing. Post-stimulation freezing may reflect the process of transferring aversive information from dPAG to higher brain structures.     

On the facilitatory effects of cognate words in bilingual speech production

There is a growing body of evidence showing that a word's cognate status is an important dimension affecting the naming performance of bilingual speakers. In a recent article, Kohnert extended this observation to the naming performance of an aphasic bilingual (DJ). DJ named pictures with cognate names more accurately than pictures with non-cognate names. Furthermore, having named the pictures in Spanish helped the subsequent retrieval (with a delay of one week between the two tests) of the same pictures' names in English, but only for pictures with cognate names. That is, there was a language transfer but only for those translation words that were phonologically similar. In this article we first evaluate the conclusions drawn from these results by Kohnert, and second we discuss the theoretical implications of the facilitatory effects of cognate words for models of speech production in bilingual speakers.     

Outcome additivity and outcome maximality influence cue competition in human causal learning

Recent research suggests that outcome additivity pretraining modulates blocking in human causal learning. However, the existing evidence confounds outcome additivity and outcome maximality. Here the authors present evidence for the influence of presenting information about outcome maximality (Experiment 1) and outcome additivity (Experiment 2) on subsequent forward blocking. The results of Experiment 3 confirm that, with outcome maximality controlled, outcome additivity affects backward blocking but not release from overshadowing. Finally, the results of Experiment 4 demonstrate that information about outcome additivity has a similar effect on forward blocking if presented after the blocking training instead of before. The results are compatible with the idea that blocking results from inferential processes at the time of testing and not from a failure to acquire associative strength during training.