Hormonal regulation of delta opioid receptor immunoreactivity in interneurons and pyramidal cells in the rat hippocampus

Clinical and preclinical studies indicate that women and men differ in relapse vulnerability to drug-seeking behavior during abstinence periods. As relapse is frequently triggered by exposure of the recovered addict to objects previously associated with drug use and the formation of these associations requires memory systems engaged by the hippocampal formation (HF), studies exploring ovarian hormone modulation of hippocampal function are warranted. Previous studies revealed that ovarian steroids alter endogenous opioid peptide levels and trafficking of mu opioid receptors in the HF, suggesting cooperative interaction between opioids and estrogens in modulating hippocampal excitability. However, whether ovarian steroids affect the levels or trafficking of delta opioid receptors (DORs) in the HF is unknown. Here, hippocampal sections of adult male and normal cycling female Sprague-Dawley rats were processed for quantitative immunoperoxidase light microscopy and dual label fluorescence or immunoelectron microscopy using antisera directed against the DOR and neuropeptide Y (NPY). Consistent with previous studies in males, DOR-immunoreactivity (-ir) localized to select interneurons and principal cells in the female HF. In comparison to males, females, regardless of estrous cycle phase, show reduced DOR-ir in the granule cell layer of the dentate gyrus and proestrus (high estrogen) females, in particular, display reduced DOR-ir in the CA1 pyramidal cell layer. Ultrastructural analysis of DOR-labeled profiles in CA1 revealed that while females generally show fewer DORs in the distal apical dendrites of pyramidal cells, proestrus females, in particular, exhibit DOR internalization and trafficking towards the soma. Dual label studies revealed that DORs are found in NPY-labeled interneurons in the hilus, CA3, and CA1. While DOR colocalization frequency in NPY-labeled neuron somata was similar between animals in the hilus, proestrus females had fewer NPY-labeled neurons that co-labeled with DOR in stratum oriens of CA1 and CA3 when compared to males. Ultrastructural analysis of NPY-labeled axon terminals within stratum radiatum of CA1 revealed that NPY-labeled axon terminals contain DORs that are frequently found at or near the plasma membrane. As no differences were noted by sex or estrous cycle phase, DOR activation on NPY-labeled axon terminals would inhibit GABA release probability equally in males and females. Taken together, these findings suggest that ovarian steroids can impact hippocampal function through direct effects on DOR levels and trafficking in principal cells and broad indirect effects through reductions in DOR-ir in NPY-labeled interneurons, particularly in CA1.     

Six year follow-up of health status changes in Danish adults with suicide tendency in 1994

The purpose of this study was to determine whether suicidal ideation and/ or suicide attempts have any long-term health effects. The relationship between suicidal thoughts and/or a previous suicide attempt in 1994 and the presence of suicidal ideation in 2000 was analyzed. We found that health status in 1994 differed significantly between the groups with and without suicidal ideation or a previous suicide attempt and that the differences persisted or even deepened (not significant) after 6 years. Further, people who had suicidal ideation or a suicide attempt in 1994 had a significantly greater risk (OR = 7.4) of having suicidal ideation in 2000 than people who had not.     

Daily Goals, Life Goals, and Worst Fears: Means, Ends, and Subjective Well-Being

This study addressed the relations among personal strivings (daily goals) and future life goals and worst fears. Eighty undergraduate participants (62 women, 18 men) listed their daily goals, their ultimate life goals, and their worst fears, and completed questionnaire measures of subjective well-being. Daily goals were content-analyzed for relevance to attaining life goals or avoiding worst fears. Daily goals that were instrumental to life goals or that avoided worst fears were rated as more important but also more difficult by participants. Working on daily goals avoiding one's worst fears was negatively related to measures of subjective well-being, controlling for daily goal progress, difficulty, ambivalence, and importance. Working on daily goals that were instrumental to one's life goals only weakly predicted well-being. The avoidance of worst fears interacted with daily goal appraisals such that individuals who experienced little progress at daily goals that served to avoid their "worst case scenario" experienced the lowest levels of subjective well-being. In addition, progress at daily goals that were relevant to accomplishing one's life goals was significantly more strongly related to subjective well-being than progress at daily goals that were unrelated to one's life goals. Results indicate that daily goals are used to enact life goals and avoid worst fears and that these means–end relations have implications for well-being.     

Novel popout without novelty

Localization of a novel word in an array with several familiar words is typically enhanced relative to localization in an all-novel array (between-array novel popout) and sometimes enhanced relative to familiar words in one-novel arrays (within-array novel popout). Christie and Klein (1996) have questioned the reality of the latter effect, suggesting that it may be an artifact of guessing bias. The present Experiment 1 replicated within-array novel popout with the novel word probed at chance (i.e., on only one quarter of trials). Experiments 2 and 3 demonstrated a similar popout effect for a categorically unrelated word among three categorically related words, despite superior performance on all-related arrays relative to all-unrelated arrays. Repetition of constant sets of words within the experimental context is therefore unnecessary for a popout effect, contrary to assertions by Johnston and Hawley (1994). Interitem associations appear to be sufficient to produce a popout effect; as such, “novel popout” appears to be a misnomer for a phenomenon that does not depend on novelty.     

The “New” democratic woman of modernity: Georgia O’Keeffe and Melanie Klein

Summary O’Keeffe put into visual language the psychic splitting that had occurred between the sexes in the United States, attempting to integrate what had formerly been separated as feminine and masculine into the female psyche. In her most important contribution, she explored presence rather than absence and opened up possibilities for thinking about openings. The concept of the female opening in particular had heretofore been assigned a negative meaning, signifying more often than not a gaping wound rather than a space and place of possibilities. Klein’s complex and evocative understanding of some of the earliest mental processes of life enabled researchers to delve into the meanings made of the presence of the mother and father and baby, in the context of the baby’s body-mind. Klein’s female or male baby desires to know from the beginning. She discovered splitting and projective identification, the development of anxiety and guilt under the aegis of these mental processes, the multiple meanings of aggression, and, significantly, she permitted the female baby’s body to have its own language. The answers provided by O’Keeffe and Klein bequeathed new possibilities for women’s self-invention and remain pivot points for female identity throughout the century, to be confronted again by the second wave of feminism beginning in the late 1960s, by ‘postfeminist’ debates, and by a challenged psychoanalysis. A more careful probing of these issues may help us to better understand our past so as to have greater resources for a more comprehensive reading of our present.     

Boundary-spanning functions served by rural school psychologists

The impact of perceived environmental demands on the roles and job satisfaction of the school psychologist in rural school systems was explored. Superintendents rated their perceptions of the community context and the psychologist's involvement in interagency linking functions. School psychologists rated their performance of boundary-spanning functions and their job perceptions. Results indicated that certain perceived environmental pressures are related to the performance of boundary-spanning activities. Those who performed boundary-spanning functions are more satisfied with their jobs and more influential in determining their roles.     

Social anxiety and problematic cannabis use: evaluating the moderating role of stress reactivity and perceived coping

Despite epidemiological reports indicating an association between social anxiety disorder (SAD) and cannabis use disorders (CUD), there is a paucity of research exploring the nature of this relationship. The present investigation examined potential moderators of this relationship that are consistent with a tension-reduction model of addiction. Specifically, physiological reactivity to stress and perceived coping with stress were evaluated as moderators of the relation between symptoms of SAD and CUD. Physiological (SCR) and subjective (perceived coping) responses to unpredictable white noise bursts were collected from non-clinical participants (n=123). Lifetime symptoms of CUD and anxiety disorders were assessed using a structured diagnostic interview. CUD symptomatology was associated with symptoms of SAD but not with symptoms of any other anxiety disorder. Only perceived coping to unpredictable stimuli moderated the relationship between SAD and CUD symptoms. Findings are discussed in the context of tension-reduction models of co-occurring social anxiety and problematic cannabis use.     

Organization of the Self-Concept and the Suppression of Self-Relevant Thoughts

The rebound effect associated with thought suppression has been found following attempts at suppressing both novel stimuli and stereotypical thoughts. However, research examining the suppression of self-relevant thoughts has been less successful in demonstrating the rebound effect. A potential factor that has not yet been fully explored in research on thought suppression is how different types of distracter thoughts may influence the ability to suppress unwanted thoughts. One type of distracter that people may use while attempting to suppress unwanted self-relevant thoughts is information related to other aspects of their lives. To the extent that these other aspects are more likely to be associated in memory with the unwanted thoughts, rebound should be more likely to occur. Thus, we expected and found that people lower in self-complexity (i.e., those with fewer self-aspects that are more interrelated with one another) revealed greater rebound following thought suppression that involved self-relevant distracting thoughts than did people greater in self-complexity. Implications of these findings for thought suppression, self-complexity, depression, and the experience of affect are discussed.     

Differences in semantic and translation priming across languages: The role of language direction and language dominance

In the present study, we examined bilingual memory organization, using the priming paradigm. Many of the previous studies in which this experimental technique has been used in the bilingual domain appear to have had several differences in methodology that have caused there to be a lot of variation in the data reported. The aim of the present work was to create an experimental situation that was well constrained so that automatic processes could be observed. In Experiment 1, Spanish-English bilinguals participated in an unmasked semantic- and translation-priming study in which a lexical decision task was used. The results revealed significant translation-priming effects in both language directions and, unexpectedly, significant semantic priming in the L2-L1 direction only. In Experiment 2, we examined semantic- and translation-priming effects with a forward mask design. The results indicated that significant priming was obtained only for translation word pairs in both language directions. These results are discussed with regard to current models of bilingual memory representation.     

Methodological considerations in performing semantic- and translation-priming experiments across languages

Research in the field of bilingualism has had as its principal aim to describe the structure and function of memory for bilingual speakers. A primary technique that has been used to examine bilingual memory is an examination of cross-language word priming (semantic and translation), using the lexical decision and pronunciation tasks. Although studies have, on occasion, revealed greater degrees of word priming from a dominant to a subordinate language, in comparison with the reverse, a careful review of the methodology that has been used reveals a number of issues that render conclusions such as this quite problematic. Parameters of concern include language proficiency, cognate status, masking, control conditions, word frequency and length, stimulus onset asynchrony, relatedness proportion, and nonword ratio. These factors are discussed, as well as recommendations for conducting future empirical research in this area of investigation.