An attempt to condition components of urine formation in dogs

Previous research on the renal conditional reflex (CR) has resulted in contradictory data. The work of Bykov with dogs and of Hoferet al. with humans indicated that a renal CR is possible, but the experiments of Corsonet al. and Watt and Gantt with dogs having normal or transplanted cervical kidneys showed no clear-cut renal CR’s. The present work supports the latter finding. Three female mongrel dogs were used. One dog (normal) had an externalized ureter; the other two (experimental) had denervated cervical kidneys (autografts). In 155 days of experiments, the investigators found diuresis to occur only on unconditional stimulus,i.e., only when a dog drank diluted milk (one part milk to four parts water)ad lib.; the dogs drank an average of between 500 and 1,100 ml. No diuresis occurred when 30 ml. of the mixture were given (conditional stimulus). Urinalysis indicated a lack of renal conditioning with respect to the excretion rates and the concentrations of creatinine and electrolytes, except for the sodium concentration in the normal dog’s urine, which decreased on bothad lib and test (30 ml) days; the volume was not affected. Conclusion: Drinking a small quantity of diluted milk as a conditional stimulus did not produce a conditional diuretic response either in the normal dog or in the two dogs with transplanted kidneys.     

Reflexology,schizokinesis and autokinesis

A resume of the history and concepts culminating in the work of Pavlov shows that there has been a gradual and slow elaboration toward a science of behavior. The past work has been oriented since the time of Locke increasingly toward the external environment. Pavlov’s theories, however, were directed toward forceswithin the nervous system. Further work from the Pavlovian Laboratories at Johns Hopkins and the V.A. (and elsewhere) indicate that the various physiological systems do not always work harmoniously, but that there is often a split in function (Schizokinesis)—changes occurring over a period of time—which do not seem to be referable to the external environment but to elaborations occurringwithin the organism (Autokinesis). These may tend toward improvement of function or, on the other hand, toward deterioration. The time has come when the progressive changes and the interrelationships of active fociwithin the organism should be considered as well as the reactivity of the individual toward the external environment.     

Effect of Person

The role of the experimenter in the behavioral study, although recognized by Darwin, Pavlov, psychiatrists and others, has not been adequately evaluated in terms of the quantitative measures of the CR. Our study in this paper concerns especially the cardiac changes induced by “Person” in normal and pathological animals—monkey, dog, cat, opossum, guinea pig and rabbit. Owing to the greater sensitivity of the cardiovascular and respiratory systems, these are more reliable and delicate measures than the usual somatic muscular ones. The Person can be used as an unconditional stimulus on the basis of which conditional reflexes are formed. Person has an especially pronounced effect on neurotic animals—shown often in a more pronounced way in the cardiorespiratory responses than in the more superficial behavioral ones. This Effect of Person may provide insights into the production of neurosis as well as some of the beneficial effects of therapy, e.g., the personal factor of the therapist.     

One-trial cardiac conditioning in dogs

Our interest in cardiovascular conditioning, particularly the fact that conditional tachycardia has been observed in many dogs after only one or two combinations of conditional and unconditional stimuli, led us to investigate conditioning using a single application of an unconditional stimulus. Initially we studied the effect of orienting stimuli (soft tones) on the heart rate in 9 dogs. After 30–100 presentations of the tones alone, each dog received on one occasion a 25-volt shock (sufficient to cause yelping and struggling) to a leg as unconditional stimulus immediately following a tone. Thereafter 30–100 additional tones were presented with no further shock. Little or no heart rate change occurred during the orienting tones (before shock). Three types of cardiac changes occurred during experimental sessions after the shock: 1) Increased heart rate during the tones in 5 dogs; 2) Generalized lowering of heart rate during all experimental sessions after shock in 4 dogs; 3) Electrocardiographic changes during tones in 3 of the dogs also showing the generalized decrease in heart rate. No motor flexion conditional reflexes developed. Tones an octave different in pitch from the one associated with the shock also caused approximately the same heart rate changes, indicating lack of differentiation. This one-trial cardiac conditioning persisted after the single conditioning trial for more than a month in 2 dogs and for at least 3 to 5 sessions in the other dogs.     

An attempt to condition extrasystoles using direct myocardial electrical stimulation as an unconditional stimulus

Conditioning of extrasystoles which were induced by direct electrical stimulation of the ventricular myocardium was attempted. It was found that this type of cardiac irregularity which is entirely peripheral in origin could not be conditioned. Other studies have shown that extrasystoles which are produced by stimulation of the central nervous system can be conditioned. The study supports the theory that the effect of stimuli which produce physiological changes at the periphery without intermediate involvement of the central nervous system cannot be conditioned.     

Interactions among chronic cold, corticosterone and puberty on energy intake and deposition

We have shown that chronic cold stress strongly interacts with corticosterone (B) to determine subsequent regulation of the hypothalamo-pituitary-adrenal (HPA) axis responses to novel stress. These studies, using the same 2 sets of rats, show that chronic cold also interacts with B and testosterone on signals of energy balance. The two groups of rats differed in weight by 20% and in age by 2 weeks (44-59 days of age). Adrenalectomized rats, replaced with varying doses of B, were exposed to cold or served as controls. Food intake and body weight during the experiments and hormones, metabolites and fat depots were measured on day 5. B, but not cold, affected food intake in the younger rats; by contrast, cold, but not B, affected food intake in the older rats. Testosterone was higher in older control rats and was markedly depressed by cold; younger rats had lower testosterone that was minimally affected by cold. Weight gain decreased in all rats at room temperature with increasing B, whereas they all lost weight in cold independently of B. Cold stimulated and B inhibited interscapular brown adipose tissue DNA content (reflecting sympathetic stimulation of thermogenesis). B stimulated insulin, whereas cold inhibited leptin and insulin; B also increased white adipose tissue weight gain in controls and inhibited its loss in cold. Leptin was unrelated to white adipose tissue depots in older control rats but was strongly related to these stores in younger rats and in all rats in cold. We conclude that: 1. By decreasing signals that act centrally to inhibit food intake (insulin, leptin and testosterone) cold allows B to stimulate food intake; 2. B inhibits weight gain although it causes accrual of fat; 3. Cold, probably through sympathetic stimulation of white adipose tissue, causes fat loss which is modulated by the inhibitory effect of B on sympathetic outflow; and, 4. The slope of the relationship between fat depot size and leptin becomes flatter in cold, possibly because of increased sympathetic outflow to these depots.     

Inhibitory deficits in reading disability depend on subtype: guessers but not spellers.

In this study, children with the guessing subtype of dyslexia (who read fast and inaccurately) were compared with children with the spelling subtype (who read slowly and accurately) on three aspects of executive functioning (EF): response inhibition, susceptibility to interference from irrelevant information, and planning. It was found that guessers were impaired in their ability to inhibit inappropriate responding on all tasks used to assess EF (the stop signal task, the Stroop task, and the Tower of London task). This raises the question of whether the specific reading disorder of guessers may be linked to the same executive deficits which underlie ADHD. In order to unite a fast/inaccurate reading style with executive deficiencies, an attempt is made to incorporate the concept of executive control into models of lexical activation.