Intracerebroventricular Administration of Mineralocorticoid Receptor Antisense Oligonucleotides Attenuates Salt Appetite in the Rat

The anterior ventral third ventricle (AV3V) region of the brain contains high concentrations of mineralocorticoid receptors (MR) and glucocorticoid receptors (GR) that are important in the maintenance of body fluid and electrolyte balance as well as other physiological processes. Daily intracerebroventricular pulse injections of MR antisense oligonucleotides significantly suppressed deoxycorticosterone acetate (DOCA) induced salt appetite in a dose-related manner. Similar administration of GR antisense or scrambled/sense oligonucleotide into the third ventricle failed to inhibit salt appetite. Salt appetite aroused after adrenalectomy was not suppressed by MR antisense oligonucleotide treatments but was suppressed by an antisense oligonucleotide directed against the angiotensin II AT1 receptor subtype. Receptor binding analysis demonstrated that MR and GR oligonucleotide treatments each reduced their respective receptor subtypes. Finally, although GR antisense oligonucleotide treatment was ineffective in suppressing DOCA-induced salt appetite, this treatment did increase stress induced corticosterone release as well as delayed the recovery of corticosterone to basal levels after stress.     

CATECHIZING THE HEAD AND THE HEART: AN INTEGRATED MODEL FOR CONFIRMATION MINISTRY

In catechesis for adolescents seeking confirmation in the Roman Catholic Church, a dualistic bias unconsciously dichotomizes objective doctrine and subjective psychology. This is problematic because if a catechist does not communicate mind‐independent truth, no seed of Catholic faith will have been planted in a student. At the same time, if a catechist does not affirm a student's subjectivity, the seed cannot find receptive soil. I believe the key to integrating these intellectual and affective elements – the head and the heart – lies in the link between what Bernard Lonergan calls authentic subjectivity and objectivity. Catechists can appeal to this link by embracing the moral responsibility to ‘know our knowing,’ becoming aware of our dualistic bias and judging our faith experiences according to objective standards of knowledge.     

The differential reinforcement of reward-produced and response-produced stimuli

In two experiments, groups received successive large-reward trials on odd-numbered days and successive small-reward trials on even-numbered days in the same gray alleyway. This produced a discrimination problem in which the memory of large reward (S^L) was reliably discriminative of large reward and the memory of small reward (S^S) was reliably discriminative of small reward. Intertrial interval (ITI) was varied both between and within groups. In the within-groups manipulation, ITI separating S+ trials differed from that separating S- trials. Experimental groups learned the discrimination, running slower to S^S (S- cue) than to S^L (S+ cue), and showed a negative contrast effect, running slower to S- than a small-reward control group. Discrimination was somewhat faster at massed than at spaced trials. The within-groups manipulation of ITI suggested that the effects of ITI were mediated by time-dependent changes in internal cues produced by reward events and by the instrumental response. The control exercised by internal cues was shown to be associative rather than nonassociative, e.g., motivational. Similarities with, and implications for, conventional brightness differential conditioning were discussed.