The effects of acute 17beta-estradiol treatment on gene expression in the young female mouse hippocampus

Previous studies have demonstrated that treatment with 17beta-estradiol (E(2)) improves both spatial and nonspatial memory in young female mice. Still unclear, however, are the molecular mechanisms underlying the beneficial effects of E(2) on memory. We have previously demonstrated that a single post-training intraperitoneal (i.p.) injection of 0.2 mg/kg E(2) can enhance hippocampal-dependent spatial and object memory consolidation (e.g., Gresack & Frick, 2006b). Therefore, in the present study, we performed a microarray analysis on the dorsal hippocampi of 4-month-old female mice injected i.p. with vehicle or 0.2 mg/kg E(2). Genes were considered differentially expressed following E(2) treatment if they showed a greater than 2-fold change in RNA expression levels compared to controls. Overall, out of a total of approximately 25,000 genes represented on the array, 204 genes showed altered mRNA expression levels upon E(2) treatment, with 111 up-regulated and 93 down-regulated. Of these, 17 of the up-regulated and 6 of the down-regulated genes are known to be involved in learning and memory. mRNA expression changes in 5 of the genes were confirmed by real-time quantitative PCR analysis, and protein changes in these same genes were confirmed by Western blot analysis: Hsp70, a heat shock protein known to be estrogen responsive; Igfbp2, an IGF-I binding protein; Actn4, an actin binding protein involved in protein trafficking; Tubb2a, the major component of microtubules; and Snap25, a synaptosome-specific protein required for neurotransmitter release. The types of genes altered indicate that E(2) may induce changes in the structural mechanics of cells within the dorsal hippocampus that could be conducive to promoting memory consolidation.     

The heritability of antisocial behavior: A meta-analysis of twin and adoption studies

In this paper, we describe a quantitative summary of 12 twin (n=3795 twin pairs and 3 adoption studies=338 adoptees) published since 1975 which provided 21 estimates of the heritability of antisocial behavior. Medium to large effect sizes were found for genetic influences across studies, with approximately 50% of the variance in measures of antisocial behavior attributable to genetic effects. Although effect sizes did not vary across different definitions of antisocial behavior (criminality, aggression, or antisocial personality), significantly larger estimates of genetic effect were found for severe manifestations of antisocial behavior. The importance of severity was further underscored by the significantly larger effects obtained in studies using clinic-referred samples compared to the effects obtained in studies using volunteer samples. Demographic characteristics of the samples did not influence effect sizes, although studies using more stringent methodology tended to find larger effects. These results must be interpreted in light of the small literature that was suitable for the meta-analysis due to numerous methodological limitations in existing studies.     

The Association between Anxiety and Psychopathy Dimensions in Children

Although several theoretical models posit that low levels of anxiety are a risk factor for psychopathy and antisocial behavior, a number of studies have reported elevated levels of anxiety among antisocial individuals. Nevertheless, most investigators in this literature have not distinguished between fearfulness and trait anxiety or attempted to separate the antisocial lifestyle dimension from the callous and unemotional dimension of psychopathy. In a study of clinically referred children (N = 143), we found that (a) measures of trait anxiety and fearlessness (low fearfulness) exhibited low correlations; (b) conduct problems tended to be positively correlated with trait anxiety, whereas callous and unemotional traits tended to be negatively correlated with trait anxiety; and (c) controlling statistically for the effects of one dimension increased the divergent correlations of the other dimension with both trait anxiety and fearful inhibition. These findings bear potentially important implications for the diagnosis and etiology of psychopathy and antisocial behavior and suggest that distinctions between trait anxiety and fearful inhibition, as well as between the two dimensions of psychopathy, may help to clarify longstanding confusion in this literature.     

The role of memory in attenuations of the suffix effect

Various properties attenuate the auditory suffix effect. These properties may improve recall by allowing the suffix to be excluded from the auditory store (or stores) used for immediate ordered recall, or they may improve recall even though the suffix is included in the auditory store. Nine items were presented, with the last item being either a suffix or another item to be recalled. In Experiment 1, the impairment in recall of the previous items produced by presenting a to-be-recalled last item in a different voice was the same as the impairment produced by presenting a suffix in a different voice. The same result was found in Experiment 2 for presenting the last item in a different spatial location and in Experiment 3 for delaying the presentation of the last item. Apparently the attenuating properties improve recall through memorial factors, and not by allowing the suffix to be excluded from the auditory store.     

Anerkennung als interaktionelles Moment der Psychoanalyse*

Nachdem in einem früheren Artikel (Daser 2003) die Theorie der Anerkennung ausführlicher entwickelt wurde, soll in dieser Arbeit dem Anerkennungsphänomen an praktisch-therapeutischen Beispielen nachgegangen werden. Zunächst wird Anerkennung als eine wertzuschreibende Handlung vorgestellt, die in Abhängigkeit vom Kontext das Selbstwertgefühl des Patienten stärkt und seine Beziehungsangst mindert, sodass er seine Abwehr reduzieren und sich dem analytischen Prozess zuwenden kann. Dabei erscheint Anerkennung einerseits als Wirkung der analytischen Methode, andererseits kann sie sich aber auch mit Interventionen verbinden, die dieser Methode zu widersprechen scheinen. Diese Interventionen erhalten damit eine die Selbsterfahrung des Patienten und damit den analytischen Prozess fördernde Wirkung. Dies wird an mehreren Beispielen ausgeführt. Darüber hinaus wird die Bedeutung der Anerkennung für Empathie hervorgehoben und am Fall eines sich zwischen Analytiker und Patient entfaltenden Spiels Anerkennung als ein Element herausgearbeitet, das den Übergang von einer Reinszenierung zu einer Neuinszenierung ermöglicht. Schließlich wird Anerkennung mit den Konzepten von Stern (et al. 2002) sowie von Weiss und Sampson (1986) in Beziehung gesetzt. Dabei zeigt sich Anerkennung als ein Element des Etwas-Mehr, das die analytische Therapie nach Stern* über die Deutung hinaus benötigt. Dabei ist Anerkennung aber nicht nur ein Adjuvans, das der emotionalen Wegbereitung der Deutung dient. Vielmehr ist existenzielle Anerkennung, um die es hier im Unterschied zum pädagogisch gemeinten Lob geht, Ergebnis einer triangulierenden Bewegung und damit der Deutung prozessual korreliert. Anerkennung und Deutung erscheinen so als sich wechselseitig ergänzende Momente eines Selbsterfahrungsprozesses, in dem Einsichts- und Beziehungsbildung nicht zu trennen sind.     

A better stopping rule for conventional statistical tests

The goal of some research studies is to demonstrate the existence of an effect. Statistical testing, withp less than .05, is one criterion for establishing the existence of this effect. In this situation, the fixedsample stopping rule, in which the number of subjects is determined in advance, is impractical and inefficient. This article presents a sequential stopping rule that is practical and about 30% more efficient: Once a minimum number of subjects is tested, stop withp less than .01 or greater than .36; otherwise, keep testing. This procedure keeps alpha at .05 and can be adjusted to fit researchers’ needs and inclinations.     

The intergenerational link to antisocial behavior: Effects of paternal contact

We investigated the relationship between paternal antisocial behavior and child conduct problems and we tested whether the degree of contact between father and child moderated the intergenerational link to antisocial behavior. Subjects were 92 children between the ages of 6 and 13 referred to an outpatient mental health clinic. There was a significant association between Antisocial Personality Disorder (APD) in fathers and a diagnosis of conduct problems in their children. However, the relationship between paternal APD and conduct problems in offspring was not dependent on whether or not the father was in the home or on the degree of paternal contact with their child. The theoretical implications of these findings for explaining the intergenerational link to antisocial behavior in terms of observational learning were discussed.