The Three Rs of Animal Research: What they Mean for the Institutional Animal Care and Use Committee and Why

The Institutional Animal Care and Use Committee (IACUC) is entrusted with assessing the ethics of proposed projects prior to approval of animal research. The role of the IACUC is detailed in legislation and binding rules, which are in turn inspired by the Three Rs: the principles of Replacement, Reduction, and Refinement. However, these principles are poorly defined. Although this provides the IACUC leeway in assessing a proposed project, it also affords little guidance. Our goal is to provide procedural and philosophical clarity to the IACUC without mandating a particular outcome. To do this, we analyze the underlying logic of the Three Rs and conclude that the Three Rs accord animals moral standing, though not necessarily “rights” in the philosophical sense. We suggest that the Rs are hierarchical, such that Replacement, which can totally eliminate harm, should be considered prior to Reduction, which decreases the number of animals harmed, with Refinement being considered last. We also identify the need for a hitherto implicit fourth R: Reject, which allows the IACUC to refuse permission for a project which does not promise sufficient benefit to offset the pain and distress likely to be caused by the proposed research.     

Quadratic Associations Between Empathy and Depression as Moderated by Emotion Dysregulation

Empathic tendencies have been associated with interpersonal and psychological benefits, but empathy at extreme levels or in combination with certain personal characteristics may contribute to risk for depression. This study tested the moderating role of cognitive emotion regulation in depression's association with empathy using nonlinear models. Young adults (N = 304; 77% female; M = 19 years) completed measures of cognitive emotion regulation strategies, depression, and affective and cognitive empathy. Individuals with good regulation had low levels of depression overall and their depression symptoms were lowest when levels of affective empathy were average. Individuals with poor regulation had high levels of depression overall, particularly when levels of empathy were moderate to high. Extremely high and low levels of cognitive empathy were associated with elevated depression, and this association was not moderated by regulation. These findings suggest tendencies to respond empathically to others’ needs is neither an adaptive nor maladaptive characteristic but rather moderate empathy, particularly in the context of good regulation, may offer the greatest protection against depression.     

Cautious Thoughts on “A Social Priming Data Set With Troubling Oddities”

Pashler, Rohrer, Abramson, Wolfson, and Harris (2016/this issue) identify a number of features of the data in Chatterjee, Rose, and Sinha (2013 Chatterjee, P., Rose, R. L., &; Sinha, J. (2013). Why money meanings matter in decisions to donate time and money. Marketing Letters, 21(2), 1–10. [Google Scholar]), particularly in Study 3, that cause them to question the scientific validity of the conclusions drawn from the data. Based on the critics’ analyses and my own investigation, I concur that Study 3 data are likely unreliable. However, Pashler et al. question the scientific validity of all three studies while providing support for their skepticism only for Study 3, clearly an overgeneralization. The processes leading to the original publication and the critics’ challenge to the original work highlight some crucial questions regarding trust, loyalty, mentorship, and oversight in multiple authored articles.     

Gender differences in salivary alpha-amylase and attentional bias towards negative facial expressions following acute stress induction

This study investigated gender differences in two key processes involved in anxiety, arousal and attentional bias towards threat. Arousal was assessed using salivary alpha-amylase (sAA), a biomarker of noradrenergic arousal and attention bias using a dot-probe task. Twenty-nine women and 27 men completed the dot-probe task and provided saliva samples before and after a stress induction [cold pressor stress (CPS) test]. Women displayed a significant increase in arousal (sAA) following the stressor compared to men, who displayed a significant reduction in arousal. Reaction time data revealed a significant avoidance of threat in women at baseline, but a significant change to an attention bias towards threat following the stressor. Men did not significantly respond to the stressor in terms of attentional bias. These findings suggest that women are more reactive to a stressor than men, and display an initial avoidance response to threat, but an attentional bias towards threat following stress.     

“Should It Be Considered Plagiarism?” Student Perceptions of Complex Citation Issues

Most research on student plagiarism defines the concept very narrowly or with much ambiguity. Many studies focus on plagiarism involving large swaths of text copied and pasted from unattributed sources, a type of plagiarism that the overwhelming majority of students seem to have little trouble identifying. Other studies rely on ambiguous definitions, assuming students understand what the term means and requesting that they self-report how well they understand the concept. This study attempts to avoid these problems by examining student perceptions of more complex citation issues. We presented 240 students with a series of examples, asked them to indicate whether or not each should be considered plagiarism, and followed up with a series of demographic and attitudinal questions. The examples fell within the spectrum of inadequate citation, patchwriting, and the reuse of other people’s ideas. Half were excerpted from publicized cases of academic plagiarism, and half were modified from other sources. Our findings indicated that students shared a very strong agreement that near verbatim copy and paste and patchwriting should be considered plagiarism, but that they were much more conflicted regarding the reuse of ideas. Additionally, this study found significant correlation between self-reported confidence in their understanding and the identification of more complex cases as plagiarism, but this study found little correlation between academic class status or exposure to plagiarism detection software and perceptions of plagiarism. The latter finding goes against a prevailing sentiment in the academic literature that the ability to recognize plagiarism is inherently linked to academic literacy. Overall, our findings indicate that more pedagogical emphasis may need to be placed on complex forms of plagiarism.     

Maternal participant experience in a South African birth cohort study enrolling healthy pregnant women and their infants

Background

Critical to conducting high quality research is the ability to attract and retain participants, especially for longitudinal studies. Understanding participant experiences and motivators or barriers to participating in clinical research is crucial. There are limited data on healthy participant experiences in longitudinal research, particularly in low- and middle-income countries. This study aims to investigate quantitatively participant experiences in a South African birth cohort study.

Methods

Maternal participant experience was evaluated by a self-administered survey in the Drakenstein Child Health Study, a longitudinal birth cohort study investigating the early life determinants of child health. Pregnant mothers, enrolled during the second trimester, were followed through childbirth and the early childhood years. Satisfaction scores were derived from the participant experience survey and quantitatively analyzed; associations between satisfaction scores and sociodemographic variables were then investigated using a linear regression model.

Results

Data were included from 585 pregnant mothers (median age 26.6 years), who had participated in the study for a median time of 16 months. Overall participant satisfaction was high (median score 51/60) and associated with increased attendance of study visits. Reasons for participating were a belief that involvement would improve their health, their child’s health or the health of family and friends. Potential reasons for leaving the study were inconvenience, not receiving clinical or study results, and unexpected changes in study visits or procedures. Variables associated with higher overall satisfaction scores were no prior participation in research, higher socioeconomic status, less intensive follow-up schedules and having experienced stressful life events in the past year.

Conclusions

Satisfaction scores were high and associated with increased visit attendance. Participants’ perceived benefits of study participation, most notably the potential for an improvement in the health of their child, were a significant motivator to enroll and remain in the study. The consistent theme of perceived health benefits as a motivator to join and remain in the study raises the question of whether participation in research results in actual improvements in health.

     

‘Language of the past’ – Exploring past tense disruption during autobiographical narration in neurodegenerative disorders

Compromised retrieval of autobiographical memory (ABM) is well established in neurodegenerative disorders. The recounting of autobiographical events is inextricably linked to linguistic knowledge, yet no study to date has investigated whether tense use during autobiographical narration is disrupted in dementia syndromes. This study investigated the incidence of correct past tense use during ABM narration in patients with Alzheimer's disease (AD, n = 10) and semantic dementia (SD, n = 10) in comparison with healthy older Controls (n = 10). Autobiographical narratives were analysed for episodic content (internal/external) and classified according to tense use (past/present). Across both patient groups, use of the past tense was significantly compromised relative to Controls, with increased levels of off‐target present tense verbs observed. Voxel‐based morphometry analyses based on structural MRI revealed differential associations between past tense use and regions of grey matter intensity in the brain. Bilateral temporal cortices were implicated in the SD group, whereas frontal, lateral, and medial temporal regions including the right hippocampus emerged in AD. This preliminary study provides the first demonstration of the disruption of specific linguistic constructs during autobiographical narration in AD and SD. Future studies are warranted to clarify at what point in the disease trajectory such deficits in tense use emerge, and whether these deficits are a product or contributing factor in memory disruption in these syndromes.     

Religiosity Reduces the Negative Influence of Injustice on Subjective Well-being: A Study in 121 Nations

National injustice has been linked to lower national happiness. We predict that national religiosity will mitigate this negative influence of injustice on happiness. We test this hypothesis analyzing national-level data from 121 nations, using a single-level moderated regression analysis. To capture various aspects of national injustice, we combine four national measures associated with injustice, namely: indexes of group grievances, political terror, rule of law, and corruption perceptions. The results show that national religiosity has a significant moderating effect on the relationship between injustice and happiness, such that higher levels of religiosity mitigate more of the negative effects of injustice on happiness than lower levels do. The results hold when religious affiliation and indexes of economic prosperity, education, and social support are controlled for. These results indicate that people in religious cultures may successfully utilize religious faith to deal with adverse conditions.     

The role of planning in different mathematical skills

Despite the fact that planning has been found to be a significant predictor of reading (particularly of reading comprehension), much less is known about its contribution to mathematics. The purpose of this study was to examine the role of two levels of planning (operation planning and action planning) in three mathematical skills (calculation fluency, math problem-solving, and math reasoning). Eighty Grade 2 children from Shanghai, China were assessed on measures of nonverbal cognitive ability (nonverbal matrices), working memory (digit span backwards and N-back), operation planning (matching numbers, planned codes, and planned search), action planning (crack the code), and mathematics (calculation fluency, math problem-solving, and math reasoning). The results of regression analyses showed that both levels of planning accounted for unique variance in mathematics over and above the effects of nonverbal cognitive ability and working memory. The effects of action planning were particularly strong in math problem-solving. These findings suggest that measures of planning could be used along with measures of working memory to detect children at-risk for mathematics disabilities and that intervention programmes targeting planning could be developed to boost children's mathematics performance.