A simple histological technique for localizing electrode tracks and lesions within the brain.

Histological procedures are necessary in brain stimulation or lesion work to determine the neural area which has been stimulated or damaged. Preparation of brain tissue often involves embedding and staining techniques that require specialized training, and the expense of a technician and a large assortment of special apparatus and supplies. In addition, the results of such techniques are unavailable for at least several days. A photographic method, which requires little special skill and a minimal amount of apparatus, is described here. Results can be available within minutes after the subject is sacrificed. This method has been shown to be adequate for the gross determination of lesion boundaries and electrode or cannula tip loci in brains of rats, cats, and squirrel monkeys.     

Discrimination learning by macaca mulatta with option to switch between S+ and S−

Three rhesus monkeys were trained to press either of two response keys. A response on the reinforcement key during presentation of the reinforced stimulus produced a sucrose pellet followed by an intertrial interval, but during presentation of the unreinforced stimulus produced only the intertrial interval. A response on the switching key changed the discriminative stimulus from reinforced to unreinforced or from unreinforced to reinforced. The reinforced stimulus was presented automatically on half the trials, but could be produced only by a switching response on the other half. Switching tended to occur in three distinct stages during acquisition of discriminative behavior. The first stage was identified as "nondiscriminative switching"; the second as "nonswitching"; and the third as "discriminative switching".     

Several methods for teaching serial position sequences to monkeys

Three keys were available for monkeys to press. The objective was to teach the animals to press the keys in sequences up to 10 members in length. With fading procedures, a light that cued the correct key at a given serial member of the sequence faded out slightly each time the animal selected it, and became slightly brighter after the animal made an error at that sequence member. The correct keys were faded out, starting from the end of the sequence and proceeding toward the beginning. With control procedures, the cue lights were turned off suddenly, rather than being faded gradually. In almost every instance, the animals learned a longer series of unlighted key positions with the fading procedures than they did when each key-light was turned off suddenly. Also, requiring the animals to press a series of keys cued by lights before they could reach the sequence members they were to learn hampered them in learning the later serial members. By using several different sequences, it was possible to replicate these findings within the individual animal.     

An inexpensive electro-fistular swivel for negative feedback control of self-stimulation

A swivel was developed for concurrent intraorganismic fluid injection and intracranial electrical stimulation of the unrestrained rat. Effects of various intragastric injections on bar-pressing maintained by electrical stimulation of the hypothalamus were studied. In some subjects, intragastric injections of either water or milk decreased the rate of responding. This decrease resulted from pauses in responding rather than from decreases in the local rates of responding. The decrement in responding occurred at a lower gastric volume during milk injection than during water injection. In other subjects, however, neither water, milk, nor 32% sucrose injections affected the rate of self-stimulation.