Caffeine deprivation state modulates expression of acquired liking for caffeine-paired flavours

Previous studies found that caffeine consumers acquired a liking for the flavour of novel caffeinated drinks when these drinks were consumed repeatedly in a caffeine-deprived, but not nondeprived, state. Expression of this acquired liking appeared acutely sensitive to current caffeine deprivation state, but the use of between-subjects designs confounded interpretation of those studies. The present study evaluated these findings further using a within-subject design, with one flavour paired with caffeine (CS + ) and the second with the absence of caffeine (CS–). During four CS + and four CS– training days, 32 moderate caffeine consumers alternatively consumed a novel flavoured drink a CS + paired with caffeine and a CS– flavour paired with placebo. Participants evaluated both drinks before and after training in two motivational states: caffeine deprived and nondeprived. As predicted, pleasantness ratings for the caffeine-paired flavour increased overall. However, this acquired liking was only significant when tested in a caffeine-deprived state. These data are consistent with a conditioned-flavour preference model and imply that expression of acquired liking for a novel caffeinated flavour depends on the need for the effects of caffeine at the time when the drink is evaluated.     

Caffeine deprivation state modulates expression of acquired liking for caffeine-paired flavours

Previous studies found that caffeine consumers acquired a liking for the flavour of novel caffeinated drinks when these drinks were consumed repeatedly in a caffeine-deprived, but not nondeprived, state. Expression of this acquired liking appeared acutely sensitive to current caffeine deprivation state, but the use of between-subjects designs confounded interpretation of those studies. The present study evaluated these findings further using a within-subject design, with one flavour paired with caffeine (CS + ) and the second with the absence of caffeine (CS-). During four CS + and four CS- training days, 32 moderate caffeine consumers alternatively consumed a novel flavoured drink a CS + paired with caffeine and a CS- flavour paired with placebo. Participants evaluated both drinks before and after training in two motivational states: caffeine deprived and nondeprived. As predicted, pleasantness ratings for the caffeine-paired flavour increased overall. However, this acquired liking was only significant when tested in a caffeine-deprived state. These data are consistent with a conditioned-flavour preference model and imply that expression of acquired liking for a novel caffeinated flavour depends on the need for the effects of caffeine at the time when the drink is evaluated.     

Facilitation and Retardation of Flavour Aversion Conditioning Following prior Exposure to the CS

Two sets of experiments on flavour aversion conditioning in rats examined the effects of the following factors: the nature of the flavour serving as the conditioned stimulus (CS), which was either a compound of monsodium glutamate, sucrose and quinine, or an elemental flavour, either sucrose or dilute acid; the amount of the CS rats consumed on a single conditioning trial-4 ml or 1 ml; and whether rats had been pre-exposed to the CS before this conditioning trial. There were three main findings. First, conditioning to an elemental CS was unaffected by the amount consumed on the conditioning trial, but conditioning to 1 ml of the compound CS was poorer than to 4 ml. Second, pre-exposure produced a latent inhibition effect if the CS was an elemental flavour, or if rats were allowed to drink 4 ml of the compound solution on their conditioning trial. But, third, pre-exposure facilitated conditioning in rats given only 1 ml of the compound solution as their CS.     

Do different types of US produce similar evaluative conditioning? An examination with contingency-awareness and cognitive load

Evaluative conditioning (EC) refers to a modulation in the liking of a neutral stimulus after it has been paired with an unconditioned stimulus (US). Previous studies have uncovered some of the mechanisms responsible for EC, but many parameters remain unknown. The study addresses two questions: Is the amplitude of EC and the impact of cognitive variables are the same for different types of US? We compared three types of US: shocks, single images and multiple semantically related images. This conditioning occurred while participants maintained a concurrent cognitive load, or not. Our results indicate that all types of US produced a significant EC of similar amplitude. Furthermore, the two cognitive variables had a similar impact across all types of US. Specifically, contingency-awareness was related to the amplitude of EC, particularly in the cognitive load group. Our results illustrate the importance of within study comparisons to assess different parameters on EC.     

Both trace and delay conditioning of evaluative responses depend on contingency awareness

Whereas previous evaluative conditioning (EC) studies produced inconsistent results concerning the role of contingency knowledge, there are classical eye-blink conditioning studies suggesting that declarative processes are involved in trace conditioning but not in delay conditioning. In two EC experiments pairing neutral sounds (conditioned stimuli; CSs) with affective pictures (unconditioned stimuli; USs), we tested whether the type of conditioning procedure affects the relationship between awareness and EC. Auditory CSs and visual USs were either overlapping and co-terminating (Delay Conditioning Group), or they were separated by a short temporal gap (Trace Conditioning Group). In both groups, contingency awareness was manipulated by varying the duration at which the US was presented during conditioning. Furthermore, in addition to a post-conditioning measure of awareness, US detectability was measured concurrently during the learning phase in Experiment 2. US exposure duration affected both measures of awareness. The data of both experiments demonstrate that EC does not occur if the USs are presented at very brief durations, but reliable EC effects can be observed at longer US exposure durations. As there were no differences between trace and delay conditioning, the data suggest that contingency awareness plays a crucial role in trace and in delay conditioning of evaluative responses. These results challenge automatic association-formation accounts of EC, but they are in line with ‘single-process’ accounts assuming EC to be dependent on conscious declarative knowledge about CS–US contingencies.     

Observational evaluative conditioning of an embedded stimulus element

Evaluative conditioning refers to the observation that the mere paired presentation of a neutral stimulus (CS) with a liked or disliked stimulus (US) may result in the neutral stimulus itself acquiring positive or negative valence. In most studies, the CS is an autonomous, invariant stimulus, and the subject directly experiences both CS and US. In this experiment, we investigated whether evaluative conditioning can be extended to a situation wherein the CS is no more than an invariant element of a complex, variable stimulus configuration, and wherein the subject experiences the CS–US co-occurrences indirectly, i.e. by observing a socius who is exposed to the CS–US pairings and facially expresses either liking or disliking the US. During acquisition, subjects watched video-taped sequences of an actor drinking a glass containing a liquid and facially expressing either liking or disliking the drink. The stimulus element which was systematically paired with the actor's facial expression of liking or disliking, was whether the glass contained a ‘foot’ or no ‘foot’ (CS), while other characteristics of the scenes were systematically varied and paired equally often with an expression of like and dislike. Next, valence ratings were obtained for pictures in which the CS element (foot/no foot) was embedded. A clear observational evaluative learning effect could be demonstrated when the feature CS was embedded in objects identical to those presented during learning, but not when it was embedded in new objects. These data demonstrate the possibility of vicarious evaluative conditioning of an embedded stimulus element, but probably at a lower level of abstraction than intended.     

Flavour liking and preference conditioned by caffeine in humans

When caffeine consumers repeatedly experience a novel flavoured drink containing caffeine, the rated pleasantness of the drink flavour increases progressively. These results could be interpreted in terms of the flavour acting as a Pavlovian conditioned stimulus (CS) predicting the consequences of caffeine ingestion. However, all studies of this phenomenon to date have used between-subjects designs, and one criticism of this is that changes in pleasantness might have arisen from nonspecific effects. A more rigorous test is to examine changes in pleasantness for two drinks, a CS+ flavour paired with caffeine and CS- paired with placebo. Accordingly, 20 moderate caffeine consumers consumed both CS+ and CS- drinks in counterbalanced order over eight conditioning trials at breakfast, with hedonic and sensory characteristics evaluated on each trial. As predicted, the rated pleasantness of the CS+ drink increased whereas pleasantness of the CS- drink did not change. Despite this, participants did not have an overall preference for the CS+ flavour posttraining. However, both those who chose the CS+ and those who chose the CS- at the end showed the same direction and rate of change in pleasantness for the two drinks during training, but spurious differences in baseline preference obscured this effect in terms of an overall change in preference. Overall these data suggest that changes in pleasantness of drinks paired with caffeine delivery are best explained in terms of Pavlovian associations between drink flavour and the postingestive effects of caffeine.     

Evaluative conditioning without directly experienced pairings of the conditioned and the unconditioned stimuli

Evaluative conditioning (EC) is the valence change of a stimulus (conditioned stimulus, CS) that is due to the previous pairing with another stimulus (unconditioned stimulus, US). We investigated whether EC can occur also when the CS–US pairings are not experienced directly by the participant but are implied by other events that the participant encounters. In two experiments, positive USs were presented in some trials and negative USs in other trials. Afterwards, participants were given information from which it was possible to conclude that CSs were covertly present during these trials. Finally, the valence of these CSs was registered using both implicit (Implicit Association Test, affective priming) and explicit measures (valence ratings). In line with the assumption that EC effects can be based on CS–US pairings that are not directly experienced, the valence of the CSs changed in the direction of the US with which they were covertly paired. This effect was observed both on explicit and on implicit measures. We argue that several aspects of our results are in line with propositional models of EC and fit less well with association formation models.     

Is evaluative conditioning really resistant to extinction? Evidence for changes in evaluative judgements without changes in evaluative representations

Evaluative conditioning (EC) is defined as the change in the evaluation of a conditioned stimulus (CS) due to its pairing with a positive or negative unconditioned stimulus (US). Although several individual studies suggest that EC is unaffected by unreinforced presentations of the CS without the US, a recent meta-analysis indicates that EC effects are less pronounced for post-extinction measurements than post-acquisition measurements. The disparity in research findings suggests that extinction of EC may depend on yet unidentified conditions. In an attempt to uncover these conditions, three experiments (N = 784) investigated the influence of unreinforced post-acquisition CS presentations on EC effects resulting from simultaneous versus sequential pairings and pairings with single versus multiple USs. For all four types of CS–US pairings, EC effects on self-reported evaluations were reduced by unreinforced CS presentations, but only when the CSs had been rated after the initial presentation of CS–US pairings. EC effects on an evaluative priming measure remained unaffected by unreinforced CS presentations regardless of whether the CSs had been rated after acquisition. The results suggest that reduced EC effects resulting from unreinforced CS presentations are due to judgement-related processes during the verbal expression of CS evaluations rather than genuine changes in the underlying evaluative representations.     

Evaluative conditioning depends on higher order encoding processes

Evaluative conditioning (EC) is commonly conceived as stimulus-driven associative learning. Here, we show that internally generated encoding activities mediate EC effects: Neutral conditioned stimuli (CS) faces were paired with positive and negative unconditioned stimuli (US) faces. Depending on the encoding task (Is CS a friend vs. enemy of US?), Experiment 1 yielded either normal EC effects (CS adopting US valence) or a reversal. This pattern was conditional on the degree to which encoding judgements affirmed friend or enemy encoding schemes. Experiments 2a and 2b replicated these findings with more clearly valenced US faces and controlling for demand effects. Experiment 3 demonstrated unconditional encoding effects when participants generated friend or enemy relations between CS and US faces. Explicitly stated friend or enemy relations in Experiment 4 left EC effects unaffected. Together, these findings testify to the importance of higher order cognitive processes in conditioning, much in line with recent evidence on the crucial role of conditioning awareness.     

Evaluative conditioning depends on higher order encoding processes

Evaluative conditioning (EC) is commonly conceived as stimulus-driven associative learning. Here, we show that internally generated encoding activities mediate EC effects: Neutral conditioned stimuli (CS) faces were paired with positive and negative unconditioned stimuli (US) faces. Depending on the encoding task (Is CS a friend vs. enemy of US?), Experiment 1 yielded either normal EC effects (CS adopting US valence) or a reversal. This pattern was conditional on the degree to which encoding judgements affirmed friend or enemy encoding schemes. Experiments 2a and 2b replicated these findings with more clearly valenced US faces and controlling for demand effects. Experiment 3 demonstrated unconditional encoding effects when participants generated friend or enemy relations between CS and US faces. Explicitly stated friend or enemy relations in Experiment 4 left EC effects unaffected. Together, these findings testify to the importance of higher order cognitive processes in conditioning, much in line with recent evidence on the crucial role of conditioning awareness.     

Aware and (dis)liking: item-based analyses reveal that valence acquisition via evaluative conditioning emerges only when there is contingency awareness

Evaluative conditioning (EC) refers to changes in the liking of an affectively neutral stimulus (the conditioned stimulus, or CS) following the pairing of that stimulus with another stimulus of affective value (the unconditioned stimulus, or US). In 3 experiments, the authors assessed contingency awareness, that is, awareness of the CS-US associations, by relying on participants' responses to individual items rather than using a global method of assessment. They found that EC emerged on contingency aware CSs only. Of note, whether the CSs were evaluated explicitly (Experiments 1 and 2) or implicitly (Experiment 3) did not make a difference. This pattern supports the idea that awareness of the CS-US associations may be required for valence acquisition via EC.     

What you see is what will change: Evaluative conditioning effects depend on a focus on valence

This study investigated whether evaluative conditioning (EC) effects depend on an evaluative focus during the learning phase. An EC effect is a valence change of an originally neutral stimulus (conditioned stimulus or CS) that is due to the former pairing with a positive or negative stimulus (unconditioned stimulus or US). In three experiments, the task focus during the conditioning phase was manipulated. Participants judged CS–US pairings either with respect to their valence or with respect to another stimulus dimension. EC effects on explicit and implicit measures were found when valence was task relevant but not when the non-valent stimulus dimension was task relevant. Two accounts for the valence focus effect are proposed: (1) An additional direct learning of the relation of CS and evaluative responses in the valence focus condition, or (2) a stronger activation of US valence in the valence focus condition compared to the non-valent focus condition.     

Reinstatement of fear in humans: autonomic and experiential responses in a differential conditioning paradigm

The present study investigated reinstatement of fear in humans using an aversive differential conditioning paradigm. Two neutral human face pictures were presented during habituation, acquisition, extinction, and postreinstatement phases. One picture served as a conditioned stimulus (CS) reinforced by an unconditioned stimulus (US) in the form of electrical stimulation (CS+) and the second picture as a control stimulus that was never reinforced (CS-). The prediction that in a reinstatement manipulation a previously extinguished fear response in humans can be reinstated in a reinstatement group by the mere presentation of three unpredicted electrical stimulations (USs) was tested. Participants in the control group were not exposed to unpredicted USs and no reinstatement effect was expected. Outcome measures included subjective US expectancy ratings and skin conductance responses. Results showed non-selective return of the fear response due to fear recovery associated with both CSs (CS+/CS-) in the reinstatement group. Unexpected fear recovery was observed for both CSs (CS+/CS-) in control participants. Results are discussed with respect to context conditioning, fear generalisation, and anxiety-related cognitive mechanisms underlying fear recovery after extinction.     

When congruence breeds preference: the influence of selective attention processes on evaluative conditioning

We investigated in two experiments whether selective attention processes modulate evaluative conditioning (EC). Based on the fact that the typical stimuli in an EC paradigm involve an affect-laden unconditioned stimulus (US) and a neutral conditioned stimulus (CS), we started from the assumption that learning might depend in part upon selective attention to the US. Attention to the US was manipulated by including a variant of the Eriksen flanker task in the EC paradigm. Similarly to the original Flanker paradigm, we implemented a target-distracter logic by introducing the CS as the task-relevant stimulus (i.e. the target) to which the participants had to respond and the US as a task-irrelevant distracter. Experiment 1 showed that CS–US congruence modulated EC if the CS had to be selected against the US. Specifically, EC was more pronounced for congruent CS–US pairs as compared to incongruent CS–US pairs. Experiment 2 disentangled CS–US congruence and CS–US compatibility and suggested that it is indeed CS–US stimulus congruence rather than CS–US response compatibility that modulates EC.     

Evaluative conditioning without directly experienced pairings of the conditioned and the unconditioned stimuli

Evaluative conditioning (EC) is the valence change of a stimulus (conditioned stimulus, CS) that is due to the previous pairing with another stimulus (unconditioned stimulus, US). We investigated whether EC can occur also when the CS-US pairings are not experienced directly by the participant but are implied by other events that the participant encounters. In two experiments, positive USs were presented in some trials and negative USs in other trials. Afterwards, participants were given information from which it was possible to conclude that CSs were covertly present during these trials. Finally, the valence of these CSs was registered using both implicit (Implicit Association Test, affective priming) and explicit measures (valence ratings). In line with the assumption that EC effects can be based on CS-US pairings that are not directly experienced, the valence of the CSs changed in the direction of the US with which they were covertly paired. This effect was observed both on explicit and on implicit measures. We argue that several aspects of our results are in line with propositional models of EC and fit less well with association formation models.     

Blocking in Human Electrodermal Conditioning

Associative blocking in human conditioning was investigated using electrodermal and self-reported US expectancy measures. Previous null results using a design reported by Lovibond, Siddle, and Bond (1988) suggested that a clearly demarcated phase structure and visual cues with semantic content may have distracted attention from the experimental contingencies. Therefore the current experiment intermixed pre-training and compound training trials and masked the transition to the test phase to remove or reduce phase boundaries. Simple coloured squares were used as CSs to reduce semantic content. A significant blocking effect was observed on both the expectancy measure and on the electrodermal measure. Both results were due to improved transfer of conditioning to the target CS in the overshadowing control group in comparison to previous experiments. The results were interpreted as providing evidence that previous null results were due to failure of transfer of learning across clearly distinct phases. There was no evidence of a dissociation between the electrodermal and self-report measures. Theoretical and procedural implications for human Pavlovian conditioning are discussed.     

Preferences surf on the currents of words: Implicit verb causality influences evaluative conditioning

It is a psychological truism that thought shapes language. However, the idea that language constrains cognition is less well understood and has been debated in philosophy, linguistic, and psychology. The goal of the present research was to investigate the influence of language, as given in linguistic categories, on the formation of evaluations in an interpersonal impression formation context. Specifically, we examined the role of different verb classes in the formation of interpersonal (dis‐)likes within an evaluative conditioning (EC) paradigm. EC refers to the change in liking in a conditioned stimulus (CS) as a result of its' pairing with an unconditioned stimulus (US). In contrast to traditional EC accounts that assume the rigid and unrestricted change in valence due to CS–US co‐occurrence, we found that EC was moderated by language, that is, by the linguistic status of the US. Theoretical implications of these findings are discussed. Copyright © 2010 John Wiley & Sons, Ltd.     

What you see is what will change: evaluative conditioning effects depend on a focus on valence

This study investigated whether evaluative conditioning (EC) effects depend on an evaluative focus during the learning phase. An EC effect is a valence change of an originally neutral stimulus (conditioned stimulus or CS) that is due to the former pairing with a positive or negative stimulus (unconditioned stimulus or US). In three experiments, the task focus during the conditioning phase was manipulated. Participants judged CS-US pairings either with respect to their valence or with respect to another stimulus dimension. EC effects on explicit and implicit measures were found when valence was task relevant but not when the non-valent stimulus dimension was task relevant. Two accounts for the valence focus effect are proposed: (1) An additional direct learning of the relation of CS and evaluative responses in the valence focus condition, or (2) a stronger activation of US valence in the valence focus condition compared to the non-valent focus condition.     

At the crossroads: Attention,contingency awareness,and evaluative conditioning

Evaluative conditioning (EC) refers to changes in the evaluation of a conditioned stimulus (CS) due to its repeated pairing with an unconditioned stimulus (US). One of the most debated topics in EC research is whether or not EC is dependent on contingency awareness. In this study, we go beyond this debate by examining whether contingency awareness mediates the impact of attentional resources and goal-directed attention on EC. Attentional resources were manipulated by presenting CSs and USs either within the same modality or in different modalities. Goal-directed attention was manipulated by asking participants to respond to the CSs or to the USs. Results indicate that the effect of goal-directed attention on EC is mediated by contingency awareness, whereas the effect of attentional resources on EC is not.