Compression and expansion of circadian rhythm in mice under long and short photoperiods

Although the functional aspects of synchronization of the circadian pacemaker by environmental light have been extensively studied, few studies have provided systematic information about the temporal organization of behavior under light-dark cycles with varying proportions of light and darkness. In the present study, the running-wheel activity profiles of mice were investigated under short, medium, and long photoperiods. The results clearly indicated that the temporal distribution of locomotor activity in mice is modulated by photoperiod. The activity profile was compressed under long photoperiods and expanded under short photoperiods. Although negative masking by light and alterations in the state of dark adaptation may have partially accounted for the phenomenon, the major mechanism seemed to be a compression and expansion of the circadian pacemaker’s cycle, as expressed in the compression and expansion of the photic phase-response curve.     

The effects of morphine on fixed-interval patterning and temporal discrimination

Changes produced by drugs in response patterns under fixed-interval schedules of reinforcement have been interpreted to result from changes in temporal discrimination. To examine this possibility, this experiment determined the effects of morphine on the response patterning of 4 pigeons during a fixed-interval 1-min schedule of food delivery with interpolated temporal discrimination trials. Twenty of the 50 total intervals were interrupted by choice trials. Pecks to one key color produced food if the interval was interrupted after a short time (after 2 or 4.64 s). Pecks to another key color produced food if the interval was interrupted after a long time (after 24.99 or 58 s). Morphine (1.0 to 10.0 mg/kg) decreased the index of curvature (a measure of response patterning) during fixed intervals and accuracy during temporal discrimination trials. Accuracy was equally disrupted following short and long sample durations. Although morphine disrupted temporal discrimination in the context of a fixed-interval schedule, these effects are inconsistent with interpretations of the disruption of response patterning as a selective overestimation of elapsed time. The effects of morphine may be related to the effects of more conventional external stimuli on response patterning.     

Scalar properties in animal timing: conformity and violations

The article reviews data from animal subjects on a range of timing tasks (including fixed-interval and temporal differentiation schedules, stimulus timing, aversive conditioning, and Pavlovian methods) with respect to conformity to the two scalar properties of timing behaviour: mean accuracy and scalar (Weberian) variance. Systematic deviations were found in data from temporal differentiation schedules, timing of very short (<100 ms) or very long (>100 s) durations, effects of "task difficulty", and some special cases where circadian and interval timing seemed to interact, or where some specific durations seemed to be timed more precisely than others. Theoretical reconciliation of some of these deviations with underlying scalar timing can be achieved, but a number of problematical cases remain unexplained.     

The effects of social defeat and other stressors on the expression of circadian rhythms

Most biological functions display a 24 h rhythm that, in mammals, is under the control of an endogenous circadian oscillator located in the suprachiasmatic nuclei (SCN) of the hypothalamus. The circadian system provides an optimal temporal organization for physiological processes and behavior in relation to a cyclic environment imposed upon organisms by the regular alternation of day and night. In line with its function as a clock that serves to maintain a stable phase-relationship between endogenous rhythms and the light-dark cycle, the circadian oscillator appears to be well protected against unpredictable stressful stimuli. Available data do not provide convincing evidence that stress is capable of perturbing the central circadian oscillator in the SCN. However, the shape and amplitude of a rhythm is not determined exclusively by the SCN and certain stressors can strongly affect the output of the clock and the expression of the rhythms. In particular, social stress in rodents has been found to cause severe disruptions of the body temperature, heart rate and locomotor activity rhythms, especially in animals that are subject to uncontrollable stress associated with defeat and subordination. Such rhythm disturbances may be due to effects of stress on sub-oscillators that are known to exist in many tissues, which are normally under the control of the SCN, or due to other effects of stress that mask the output of the circadian system. These disturbances of peripheral rhythms represent an imbalance between normally precisely orchestrated physiological and behavioral processes that may have severe consequence for the health and well being of the organism.     

Movement aftereffects contingent on color,intensity, and pattern

We have found contingent movement aftereffects (CMAEs) lasting several days, contingent upon the color, intensity, and stripe width of moving patterns. Ss adapted for 10 min to a patterned disk rotating clockwise under red light, alternating every 10 sec with counterclockwise under green light. When stopped, the disk then appeared to rotate counterclockwise under red light and clockwise under green light. The effect lasted only a second or two, reappearing each time the field’s color was changed. But it increased in strength over the first 1/2 hand was still present 1 or 2 days later. Color transposition effects were found: after adaptation to red-clockwise (long wavelength) alternating with green-counterclockwise (short wavelength), a stationary yellow (long wavelength) test field appeared to rotate counterclockwise and a blue (short wavelength) field appeared to rotate clockwise. Relative, not absolute, color of the test triggered the CMAE. Similar CMAEs and transposition effects were produced by pairing direction of movement with intensity, with width of moving stripes and with orientation of a stationary grating projected onto a rotating patterned disk.     

Scalar properties in animal timing: Conformity and violations

The article reviews data from animal subjects on a range of timing tasks (including fixed-interval and temporal differentiation schedules, stimulus timing, aversive conditioning, and Pavlovian methods) with respect to conformity to the two scalar properties of timing behaviour: mean accuracy and scalar (Weberian) variance. Systematic deviations were found in data from temporal differentiation schedules, timing of very short (<100 ms) or very long (>100 s) durations, effects of “task difficulty”, and some special cases where circadian and interval timing seemed to interact, or where some specific durations seemed to be timed more precisely than others. Theoretical reconciliation of some of these deviations with underlying scalar timing can be achieved, but a number of problematical cases remain unexplained.     

Social and environmental factors influence the suppression of pup-directed aggression and development of paternal behavior in captive meadow voles (Microtus pennsylvanicus)

During summer, female meadow voles (Microtus pennsylvanicus) maintain territories and males do not engage in paternal care. As day length shortens, territories dissolve and males nest with females and young. Because paternal behavior has never been studied in free-living meadow voles during colder months or in the laboratory under short photoperiods, the authors examined whether males housed in short day (SD) lengths exhibited more frequent or better quality paternal behavior than males housed in long day (LD) lengths. Sexually and parentally inexperienced (naive) SD males exhibited proportionally more and qualitatively better paternal care than naive LD males. SD males were more responsive than LD males to classic social cues associated with prepartum aggression inhibition and paternal onset. SD sires also displayed qualitatively better paternal behavior than LD sires. These data suggest that meadow vole paternal state is regulated by specific social and environmental cues that may contain reliable information about ecological conditions that favor paternal care.     

Discrimination of visually perceived intervals of time

The discrimination of short intervals of time, demarcated by a foveally presented spatially distinct double pulse of light, was studied under several conditions of pulse intensity, angular diameter, and duration. We defined temporal acuity as a measure of discrimination capacity in terms of d′ values. It is shown that the acuity mechanism uses largely integrated information—in the spatial and temporal domain, up to at least 56′ and 32 msec, respectively. Acuity increases slightly with increasing integrated pulse energy, but seems quite independent of the presence of an adapting field of appreciable brightness. Studies on the effect of the foveal site aimed at by the pulses of light have shown that temporal projections lead to significantly poorer acuity values than nasal projections. Monoptic and dichoptic stimulation, however, are fully equivalent.     

Changing Sensitivity to Duration in Human Scalar Timing: An Experiment, a Review, and Some Possible Explanations

Evidence from a number of studies of human timing, using temporal generalization and bisection tasks, suggests more sensitive behavioural adjustment to presented durations under conditions in which the timing task demands discriminations between more closely spaced stimuli. An experiment using temporal generalization demonstrated this effect, as discrimination between a 600-msec standard duration and non-standard stimuli both shorter and longer than 600 msec was better when non-standard stimuli were more closely spaced around 600 msec. A review showed similar effects in other temporal generalization tasks and in a number of bisection studies, where time discrimination improved as the ratio of the long and short standards on the bisection task decreased. A standard model of human temporal generalization explained the experimental data in terms of a decrease in the response threshold under more difficult conditions, rather than changes in the representation of the standard duration. On the other hand, data from bisection could be modelled by assuming the contrary; that representations of the short and long standards of the task were more precise under the more difficult conditions. Explanations of some of these effects in terms of attention to duration and/or arousal-induced changes in the speed of an internal clock were discussed.     

Evidence for separate control of estrous and circadian periodicity in the golden hamster.

To study the relationship between estrous and circadian periodicity, we investigated the period of the estrous cycle in two types of female golden hamsters: normals (circadian period approximately 24 h) and tau mutants (circadian period approximately 20 h). Records of running wheel activity, general locomotor activity, body temperature, vaginal secretion, and sexual receptivity of hamsters kept under constant lighting conditions indicated an estrous period of approximately 96 h for both groups of animals. The fact that animals with different circadian periods have the same estrous period suggests the existence of separate mechanisms in the control of circadian and estrous periodicity. Circadian periodicity is determined by a pacemaker located in the suprachiasmatic nuclei, whereas estrous periodicity is determined by positive and negative feedback loops involving the hypothalamus, pituitary, and gonads. Coupling of the two mechanisms takes place under at least some conditions, but additional research is necessary to elucidate the mechanisms by which this is accomplished.     

Light-dark variation and changes across the lactational period in the behaviors of undisturbed mother and infant guinea pigs (Cavia porcellus)

Lactating guinea pigs (Cavia porcellus) and their litters were observed by videophotography across the light/dark cycle at 1, 11, 21, and 31 days postpartum. The highest level of behavioral activity was seen in the dark, particularly in the hour after light offset. This circadian pattern was evident from Day 1 in mothers and from Day 11 in pups. Contact between mothers and pups was inversely related to activity, occurring more frequently during light. Maternal grooming of pups occurred on Day 1 and then declined: self-grooming by pups increased across days. Intake of solid food and water by pups occurred on Day 1 and increased thereafter. A nearly complete transition from nursing to independent ingestion was observed between 21-31 days of age. Overall, we document several ontogenetic changes in young guinea pigs and demonstrate that under laboratory conditions mother and infant guinea pigs exhibit a nocturnal activity pattern.     

Post-light potentiation at type B to A photoreceptor connections in Hermissenda

We investigated whether the long (approximately 30-s) or short (approximately 3-s) light stimuli that have been used during behavioral training would induce post-light potentiation (PLP) at the type B to A photoreceptor connections of the isolated nervous system of Hermissenda. We found that a single approximately 30-s light step induced PLP at these connections relative to both pre-light baseline and seawater control preparations, as did a series of nine short (approximately 3-s) light steps. In addition, a 30-s step of depolarization-elicited type B cell activity induced potentiation comparable to that induced by a approximately 30-s light step, indicating that light-elicited type B cell activity contributes to the induction of PLP. By contrast, even though a series of short (3-s) light steps induced potentiation, short steps of depolarization-evoked type B cell activity did not. Hence, light-evoked processes other than type B cell depolarization or activity (e.g., perhaps intracellular Ca2+ release) also contribute to the induction of PLP. Further results suggest that these other light-evoked processes interact nonadditively with type B cell activity to generate PLP. Some but not all instances of synaptic potentiation were accompanied by various changes in parameters of type B cell action potentials and afterhyperpolarizing potentials, suggesting diverse underlying mechanisms, including increases in neurotransmitter release. Given that the type A cells have been proposed to polysynaptically excite the motor neurons that drive phototaxis, a light-evoked potentiation of synaptic strength at the inhibitory type B to A photoreceptor connections may play a mechanistic role in light-elicited nonassociative learning.     

On the use of microwave radar devices in chronobiology studies: An application with<Emphasis Type="Italic">Periplaneta americana</Emphasis>

Modified motion detectors can be used to monitor locomotor activity and measure endogenous rhythms. Although these devices can help monitor insects in their home cages, the small size of the animals requires a very short wavelength detector. We modified a commercial microwave-based detection device, connected the detector’s output to the digital input of a computer, and validated the device by recording circadian and ultradian rhythms.Periplaneta americana were housed in individual cages, and their activity was monitored at 18°C and subsequently at 28°C in constant darkness. Time series were analyzed by a discrete Fourier transform and a chi-square periodogram. Q₁₀ values and the circadian free-running period confirmed the data reported in the literature, validating the apparatus. Moreover, the spectral analysis and periodogram revealed the presence of ultradian rhythmicity in the range of 1–8 h.     

Illusory recouplings of onsets and terminations of glide tone components

We present a new auditory illusion, the gap transfer illusion, supported by phenomenological and psychophysical data. In a typical situation, an ascending frequency glide of 2,500 msec with a temporal gap of 100 msec in the middle and a continuously descending frequency glide of 500 msec cross each other at their central positions. These glides move at the same constant speed in logarithmic frequency in opposite directions. The temporal gap in the long glide is perceived as if it were in the short glide. The same kind of subjective transfer of a temporal gap can take place also when the stimulus pattern is reversed in time. This phenomenon suggests that onsets and terminations of glide components behave as if they were independent perceptual elements. We also find that when two long frequency glides are presented successively with a short temporal overlap, a long glide tone covering the whole duration of the pattern and a short tone around the temporal middle can be perceived. To account for these results, we propose an event construction model, in which perceptual onsets and terminations are coupled to construct auditory events and the proximity principle connects these elements.     

Temporal compression in episodic memory for real-life events

Remembering an event typically takes less time than experiencing it, suggesting that episodic memory represents past experience in a temporally compressed way. Little is known, however, about how the continuous flow of real-life events is summarised in memory. Here we investigated the nature and determinants of temporal compression by directly comparing memory contents with the objective timing of events as measured by a wearable camera. We found that episodic memories consist of a succession of moments of prior experience that represent events with varying compression rates, such that the density of retrieved information is modulated by goal processing and perceptual changes. Furthermore, the results showed that temporal compression rates remain relatively stable over one week and increase after a one-month delay, particularly for goal-related events. These data shed new light on temporal compression in episodic memory and suggest that compression rates are adaptively modulated to maintain current goal-relevant information.     

Averaging saccades are repelled by prior uninformative cues at both short and long intervals

When two spatially proximal stimuli are presented simultaneously, a first saccade is often directed to an intermediate location between the stimuli (averaging saccade). In an earlier study, Watanabe (2001) showed that, at a long cue–target onset asynchrony (CTOA; 600 ms), uninformative cues not only slowed saccadic response times (SRTs) to targets presented at the cued location in single target trials (inhibition of return, IOR), but also biased averaging saccades away from the cue in double target trials. The present study replicated Watanabe's experimental task with a short CTOA (50 ms), as well as with mixed short (50 ms) and long (600 ms) CTOAs. In all conditions on double target trials, uninformative cues robustly biased averaging saccades away from cued locations. Although SRTs on single target trials were delayed at previously cued locations at both CTOAs when they were mixed, this delay was not observed in the blocked, short CTOA condition. We suggest that top-down factors, such as expectation and attentional control settings, may have asymmetric effects on the temporal and spatial dynamics of oculomotor processing.     

Grammatical aspect,lexical aspect,and event duration constrain the availability of events in narratives

The present study investigates how readers’ representations of narratives are constrained by three sources of temporal information; grammatical aspect, lexical aspect, and the duration of intervening events. Participants read short stories in which a target event with an intrinsic endpoint or not (lexical aspect: accomplishments/activities) was described as ongoing or completed (grammatical aspect: imperfective/perfective). An intervening sentence described either a long or short duration event before the target situation was reintroduced later in the story. The electroencephalogram time-locked to the reintroduction of the target event elicited a larger N400 for perfective versus imperfective accomplishments, and this effect occurred only after short intervening events. Alternatively, the N400 to targets in the activity condition did not vary as a function of grammatical aspect or duration of intervening events. These results provide novel insight into how the temporal properties of events interact to constrain the availability of concepts in situation models.     

Modality effects in the coding reproduction of rhythms

The temporal coding assumption is that time of presentation is coded more accurately for auditory events than for visual events. This assumption has been used to explain the modality effect, in which recall of recent auditory events is superior to recall of recent visual events. We tested the temporal coding assumption by examining the coding and reproduction of quintessentially temporal stimuli-rhythms. The rhythms were produced by sequences of short and long auditory stimuli or short and long visual stimuli; in either case, the task was to reproduce the temporal sequence. The results from four experiments demonstrated reproduction of auditory rhythms superior to that of visual rhythms. We conclude that speech-based explanations of modality effects cannot accommodate these findings, whereas the findings are consistent with explanations based on the temporal coding assumption.     

Object salience is transiently represented whereas object presence is not: evidence from temporal order judgment

We investigated the temporal dynamics in the processing of object salience and object presence by using a temporal-order-judgment task. In two experiments, observers were presented with displays containing one or two orientation singletons embedded in a background of homogeneously oriented lines. Observers had the task to indicate the temporal order of two colour changes which occurred either at two singleton locations differing in salience (experiment 1) or at two locations differing in singleton presence (experiment 2). Singleton displays were presented for a short or a long duration prior to the colour changes. The results demonstrated that salience affects the perceived temporal order of two colour changes more strongly after a short than after a long presentation duration (experiment 1). Singleton presence affects the perceived temporal order of two colour changes equally after a short and a long presentation duration (experiment 2). The results suggest that object salience and object presence are differently represented in the visual system.     

Forgetting at short term: When do event-based interference and temporal factors have an effect?

Memory tasks combining storage and distracting tasks performed at either encoding or retrieval have provided divergent results pointing towards accounts of forgetting in terms of either temporal decay or event-based interference respectively. The aim of this study was to shed light on the possible sources of such a divergence that could rely on methodological aspects or deeper differences in the memory traces elicited by the different paradigms used. Methodological issues were explored in a first series of experiments by introducing at retrieval computer-paced distracting tasks that involved articulatory suppression, attentional demand, or both. A second series of experiments that used a similar design was intended to induce differences in the nature of memory traces by increasing the time allowed for encoding the to-be-remembered items. Although the introduction of computer-paced distracting tasks allowed for a strict control of temporal parameters, the first series of experiments replicated the effects usually attributed to event-based interference. However, deeper encoding abolished these effects while time-related effects remained unchanged. These findings suggest that the interplay between temporal factors and event-based interference in forgetting at short term is more complex than expected and could depend on the nature of memory traces.