Visual reinforcement in the female Siamese fighting fish, Betta splendens

Operant conditioning with Betta splendens (Bettas) has been investigated extensively using males of the species. Ethological studies of female Bettas have revealed aggressive interactions that qualitatively parallel those between male Bettas. Given these similarities, four experiments were conducted with female Bettas to examine the generality of a widely reported finding with males: mirror-image reinforcement. Swimming through a ring was reinforced by a 10-s mirror presentation. As with males, ring swimming was acquired and maintained when mirror presentations were immediate (Experiments 1, 2, and 3) and delayed (Experiment 4). The failure of conventional extinction (Experiments 1 and 2) and response-independent mirror presentations (Experiment 3) to maintain responding confirmed the reinforcing properties of mirror presentation. These results extend previous findings of mirror images as reinforcers in males of the same species and illustrate a complementarity between behavioral ecology and the experimental analysis of behavior.     

Effects of pH on the rate of aggressive display for mirror image reinforcement in siamese fighting fish (Betta splendens)

A hypothesis suggesting that fluctuations in hydrogen ion (pH) intensity may suppress the rate of aggressive display in Betta splendens was offered. The effect of pH upon the rate of ring swimming for aggression-releasing mirror image presentation was therefore investigated under pH intensities of 5.0, 7.0, and 9.0 in a steady-state design. The results indicated no significant variation in the rate of response over pH levels. Two possible mechanisms of pH resistance are discussed.     

Effects of trophic poisoning with methylmercury on the appetitive elements of the agonistic sequence in fighting-fish (Betta splendens)

The aggressive display in Betta splendens is particularly prominent, and vital to its adaptation to the environment. Methylmercury is an organic variation of Hg that presents particularly pronounced neuro-behavioral effects. The present experiments aim to test the effect of acute and chronic poisoning with methylmercury on the display in Bettas. The animals were poisoned by trophic means in both experiments (16 ug/kg in acute poisoning; 16 ug/kg/day for chronic poisoning), and tested in agonistic pairs. The total frequency of the display was recorded, analyzing the topography of the agonistic response. The methylmercury seems to present a dose- and detoxification-dependent effect on these responses, with a more pronounced effect on motivity in acute poisoning and on emotionality in the chronic poisoning. It is possible that this effect could be mediated by alteration in the mono-amino-oxidase systems.     

Response acquisition by Siamese fighting fish (Betta splendens) with delayed visual reinforcement

Male Siamese fighting fish, Betta splendens, swam through a ring in an aquarium, breaking a photocell beam and initiating an unsignaled, resetting delay interval. Following delays of 0 s, 10 s, or 25 s, a 15-s mirror presentation released an aggressive display by the fish. Swimming through the ring increased in the absence of either a period of acclimatization to the reinforcer (analogous to magazine training when appetitive reinforcers are used) or explicit training of the response by the experimenters. Response rates were a decreasing function of delay duration. Other fish exposed to a schedule of response-independent mirror presentations failed to acquire and maintain the response. The results demonstrate the robustness and generality of the phenomenon of response acquisition with delayed reinforcement. They further qualify earlier observations about behavioral mechanisms involved in the phenomenon.     

Effect of telencephalon ablation on the reinforcing and eliciting properties of species-specific events in Betta splendens

In male Betta splendens, aggressive behavior is drastically attenuated following telencephalon ablation. Because instrumental training and Pavlovian conditioning experiments with intact fish have suggested that associative factors may play an important role in the performance of agonistic behaviors, the effect of ablation on instrumental learning and Pavlovian conditioning was studied. In Experiment 1, ablation had no effect on the learning of the instrumental tunnel-swimming response reinforced by mirror presentation (i.e., viewing a conspecific), although the mirror presentations in yoked-control groups elicited fewer responses in ablates than in normal and sham-operated control fish. Yoked controls further established that instrumental responding was maintained by the reinforcement contingency and was not merely the result of increased motor activity. Experiment 2 studied Pavlovian conditioning of the components of the agonistic display. Unconditioned fin erection, gill erection, and tail beating (i.e., unconditioned responses, URs) to the mirror US all were less frequent in ablates than in normals or shams. Of these, only gill cover erection showed evidence of true conditioning (i.e., conditioned responses; CRs) in which responses to the conditioned stimulus (CS) are due to the pairings of CS and US (unconditioned stimulus). However, ablates suffered no impairment of conditioned gill erections. Ablates performed fewer fin erections to the CS; however, fin erection responses were not due to CS-US pairings but were attributable to pseudoconditioning. These results are related to hypotheses postulating the involvement of learning mechanisms in ablation-produced deficits and normal aggressive behavior.     

Using visual reinforcement to establish stimulus control of responding of Siamese fighting fish (Betta splendens)

Stimulus control of ring swimming was studied with male Siamese fighting fish (Betta splendens) using 2-component multiple schedules in which the components were correlated with the presence or absence of air bubbles in the water. In Experiment 1, either response-independent mirror presentations or extinction was juxtaposed with immediate response-dependent mirror presentations. Rates of ring swimming generally were higher with immediate reinforcement than with either response-independent mirror presentations or extinction. In Experiment 2, different durations of response-dependent mirror presentations were juxtaposed. Generally, higher rates of ring swimming occurred with 15-s than with 0-, 1-, or 3-s durations. Results demonstrate that stimulus control of responding can be established with these fish under several conditions of differential reinforcement.     

Acute and chronic effects of cocaine on extinction-induced aggression.

Pigeons worked individually in a chamber containing a response key and a mirror. Pecking on the key was controlled by a multiple schedule in which a brief period of continuous food reinforcement alternated with a 5-minute period of extinction. Under baseline conditions, aggressive behavior (responding on the mirror) occurred at the onset of each extinction period. In Experiment I (acute drug administration), the aggressive behavior was decreased by doses of cocaine that had little or no effect on key pecking. Such food-reinforced responding was disrupted, however, by higher doses of cocaine. An attempt to mimic the disruptive drugs effects by a prefeeding manipulation was unsuccessful. In Experiment II (chronic drug administration), some tolerance developed to the disruptive effects of cocaine on the food-reinforced responding, except at the highest dose tested. There was no clear-cut indication of tolerance to the initial effect of cocaine on the aggressive behavior at any dose.     

Sex reversal in female Betta splendens as a function of testosterone manipulation and social influence

In Experiment 1, female Betta given daily injections of testosterone (T) for 9 weeks acquired anatomical features characteristic of males as indicated by changes in fin length, body coloration, and gonadal morphology. These findings suggested that a potential for sex reversal exists in females of this species. In Experiment 2 we measured changes in aggressive behavior during testosterone-induced anatomical changes. Aggression decreased toward females and increased toward males as treatment with T progressed. The final displays of aggressive behavior and anatomical characteristics of fish injected with T resembled those of typical males. In Experiment 3, female Betta primed with T injections for 3 or 6 weeks and permitted to interact socially with females continued to display characteristics of sex reversal after T supplementation ceased. Sex reversal in isolated fish injected with T for 3 or 6 weeks was not sustained, and fish receiving only the control vehicle showed negligible change in both the isolated and community conditions. We discuss the results in terms of similarities with the sex change process found in isolated communities of coral reef fish.     

Decreased aggressive and locomotor behaviors in Betta splendens after exposure to fluoxetine

The failure of sewage treatment plants to remove pharmaceuticals such as fluoxetine from waste water has become a concern given that these products are being detected in the surface waters of many countries of the world. The effects of fluoxetine in sub-lethal doses on the neural systems and behaviors of aquatic life are worthy of investigation. This study investigated the effects of sub-lethal amounts fluoxetine dissolved in water on the aggressive and locomotor behaviors of 44 male Betta splendens. Fish treated with 705 microg/l of fluoxetine and 350 microg/l of fluoxetine generally demonstrated significant decreases in locomotion and number of aggressive attacks compared to 0 microg/l of fluoxetine (controls) on Days 11 and 19 of drug exposure and persisted for at least 13 days after removal of fluoxetine. Consistent with decreases in the number of aggressive attacks, there was a significant increase in aggression-response time to a perceived intruder for treated males on Days 11 and 19 and persisted for 6 days following removal of fluoxetine. However, the differences in aggressive and locomotor behaviors seen in the fluoxetine-treated groups were indistinguishable from controls three weeks following drug removal.     

Visual-reinforcer color-and operant behavior in Siamese fighting fish

Male Siamese Fighting Fish (Betta splendens) were conditioned to emit an operant response sequence reinforced by presentation of a model of a male Siamese Fighting Fish in aggressive display. Operant response rate varied as a function of the color of the model with respect to the color of the subject.     

Social chemosignals in five Belontiidae (Pisces) species

Approach behaviors toward conspecific chemical stimuli of the opposite sex were examined in five Belontiidae species: Betta splendens, Macropodus opercularis, Colisa labiosa, C. lalia, and Trichogaster trichopterus. Approach was measured by (a) preference for section 1 of a three-section tank, which contained a vertical tube that introduced the stimulus water, and (b) occupancy of the tube. Experiments 1A and 1B showed that (a) approach behaviors were displayed by the isolated male Betta only to ripe-female stimulus water, (b) group-housed males of the remaining four species were not attracted to female-conditioned water, and (c) socially isolated males of these four species preferred section 1 during presentation of either ripe- or nonripe-female-inhabited water but occupied the tube only during exposure to ripe-female-conditioned water. The findings of Experiment 2A were that (a) the female Betta, regarless of physiological state, showed approach behaviors to male-inhabited water and (b) only ripe females of the remaining species indicated a preference for section 1 during male-water exposure but performed no tube entries. Results of Experiment 2B indicated that social isolation of the females, especially ripe females, facilitated their approach behaviors.     

Intermittent punishment of human responding maintained by intermittent reinforcement

To determine the effects of variable-interval shock punishment on behavior maintained by variable-interval and variable-ratio reinforcement, human subjects' key-pressing behavior was reinforced with money on a four-component multiple schedule. Components 1 and 2 were variable-interval 30-sec, and Components 3 and 4 were variable-ratio 210. After responding was stabilized, response-contingent electric shock was scheduled on a variable-interval 10-sec schedule during the second and fourth components of each cycle. Subjects instructed as to the reinforcement contingencies showed gradually increasing suppression of variable-interval responding at increasing shock intensities and either very high or very low rates of variable-ratio responding at higher intensities. Minimally instructed subjects showed suppression at higher shock intensities, but no clear differential suppression as a function of reinforcement schedule. Recovery from initial suppression was observed within sessions.     

Brief report: Non-intrusive operant analysis of aggressive behavior in persons with mental retardation

A procedure for operant analysis of aggressive behavior was derived from multi-element assessment methods that have been previously applied to both self-injurious and stereotypic responding. Analog sessions included No Interaction Baseline, Social Disapproval, Demand, Social Reinforcement, and Discriminated Extinction conditions, repeatedly presented within a multi-element design. Three men with differing severity of mental retardation, communicative deficits and aggressive behavior were exposed to multiple brief analog sessions embedded within their normal meal-time routines in group home settings. Mean frequencies of aggressive behavior and concurrent eating indicate that, while aggressive behavior was negatively reinforced in all three participants, both social and non-social contingencies were also prominent. The same contingencies which have been demonstrated to maintain self-injury and stereotypic behavior can therefore also maintain aggression. Results suggest the feasibility of conducting relatively non-intrusive analog assessments in community residences, and support the-extension of functional analysis across differing classes of aberrant behavior.     

On the choice between eating and aggressive display in the Siamese fighting fish (Betta splendens)

In Expt 1, male Betta splendens were given a choice between food and a mirror image in a T-maze. All fish consistently preferred food. In Expt 2, the choice of fish that lived in the T-maze for the duration of the experiment was compared with the choice of fish that were transferred to the T-maze for each experimental session. Fish that lived in the T-maze consistently preferred food, but fish that were transferred showed a shift in preference toward mirror image. Differential inhibition of hunger and aggression by fear is suggested as the cause of the preference shift.     

Effects of toy guns and airplanes on children's antisocial free play behavior.

The effects of toy guns and toy airplanes on children's antisocial (aggression and rule-breaking) behavior was investigated in two settings (ten children in Study I and 13 in Study II) during 30-min free play sessions. Four-and 5-year-old children were observed during 15–16 free play sessions. During some sessions the children played either with (novel, aggressive) toy guns or with (novel, nonaggressive) toy airplanes in addition to their usual toys. In both studies, the toy guns treatment produced a reliably higher rate of antisocial behavior than the average of the toy airplaines and the usual toys. The novel-nonaggressive toy airplanes also increased the rate of antisocial behavior as compared to usual toys in Study II. The results were related primarily to Berkowitz's and Bandura's analyses of aggressive behavior, but they were related also to other theoretical models which predict stimulus control of behavior.     

Display variability and spatial organization as contributors to the pigeon's discrimination of complex visual stimuli

Three experiments assessed the contributions of display variability and spatial organization to the pigeon's discrimination of 16-icon visual displays. After training to discriminate 4 x 4 arrays of same and different computer icons, 4 pigeons were shown testing displays that systematically manipulated the variability of the depicted icons and their spatial organization on the display screen. Display variability and spatial organization each reliably controlled the pigeon's behavior. These seemingly separate effects could be collectively explained by the pigeon's discriminating the amount of variability or entropy in localized regions of the display.     

SOLITARY TOY PLAY AND TIME OUT: A FAMILY TREATMENT PACKAGE FOR CHILDREN WITH AGGRESSIVE AND OPPOSITIONAL BEHAVIOR

The behavior of four boys, 5 to 8 years of age, who were referred for a number of oppositional, rule violating, and aggressive behaviors, was assessed by direct observation and parent reports. Following baseline measurement, several interventions were successively applied to each child's behavior. Use of a social play contract to reduce problem behaviors by teaching appropriate social behaviors resulted either in no improvement or in worsening of the problem behaviors in observations made when the contract was not in effect. Changing the contract behavior to solitary toy play resulted in reduced oppositional behavior during the observation sessions, fewer reports from the parents of low-rate problem behaviors, and improvements in the parents' attitudes toward the children. However, these changes during the observation sessions were shortlived, because the data on these measures began to show a reversal during later sessions. Inclusion of a time-out contingency with the solitary play contract recovered the earlier improvements in the children's behavior during observation sessions and the parents' reports. These results gave support to the view that for children whose behavior is severely oppositional and aggressive, a treatment approach emphasizing productive, solitary behaviors may be superior to one stressing appropriate social interaction. However, a combined strategy of reinforcement for solitary play and punishment for problem behaviors appears necessary to ensure more durable treatment effects.     

Responses to mirror-image stimulation in jungle crows (Corvus macrorhynchos)

Four jungle crows (Corvus macrorhynchos) were exposed to a mirror placed either vertically or horizontally. The most frequently observed behaviors were pecking at the mirror and wing flapping when looking toward the mirror. These behavior patterns, which were only rarely observed when the mirror was reversed, can be interpreted as aggressive behaviors against a conspecific. The vertical mirror evoked the behaviors more often than the horizontal mirror. The present results suggest that crows perceive their mirror image as an image of a conspecific, not as their own. Received: 16 December 1999 / Accepted after revision 29 January 2000     

Group play therapy for Japanese children who stutter

Twenty-one sessions of group play therapy were conducted with children who stutter in order to improve their stuttering and to promote their development in social adaptation. Follow-up surveys were made 6 mo and 5 yr after therapy.As therapy progressed, a definite process of change was observed in each child, from periods of anxiety, appearance of aggressive behavior, frequent occurence of aggressive behavior, to a decrease in aggressive behavior. After the appearance of aggressive behavior, a fluctuation in stuttering occured as cooperative play with other children was established. This was followed by a decrease in stuttering. The appearance of aggressive behavior during therapy is thought to be of major significance for the disappearance of stuttering and for interpersonal relationships.A follow-up survey showed that both stuttering and social adjustment had improved, indicating the effectiveness of therapy.     

Effect of various discriminative stimuli on choice behavior in male siamese fighting fish (Betta splendens)

The current experiment was an exploratory study empirically comparing three discrimination methodologies proposed for use in choice preparations with food rewards. Subjects were thirty-five, healthy, adult male Siamese fighting fish (Betta splendens). Subjects were randomly assigned to one of three discrimination groups: a Direction group (using left or right as discriminative stimuli), a Color group (using red or green as discriminative stimuli), and a Bubble group (using the presence or absence of air bubbles as discriminative stimuli). For all three discrimination groups, subjects chose between one or three food pellets in a submerged T-maze. The results from the experiment indicated a statistically significant preference for the three pellets of food over one pellet of food only for the Bubble group. Of particular note is the effect size and observed power obtained for the Bubble group, which was the only group which supported a reasonably powerful test for discrimination, given a sample size of 12 subjects and 0.94 effect size.