CyclinD1、VEGF、Flt-1在肝细胞癌中的表达及临床意义

探讨肝细胞癌( Hepatocellular carcinoma,HCC)、胆管癌、肝硬化及正常肝组织中周期素D1 (CyclinD1)、血管内皮生长因子(VEGF)及血管内皮生长因子受体(Flt-1)蛋白表达的意义及其与肝癌生物学行为关系.对52例符合要求的HCC组织、10例胆管癌组织、10例肝硬化组织及10例正常肝组织采用免疫组化S-P法检测CyclinD1、VEGF及Flt-1蛋白.CyclinD1、VEGF及Flt-1在HCC的强阳性表达率分别为55.8％、55.8％及67.3％,均显著高于胆管癌、肝硬化及正常肝组织(P＜0.001).CyclinD1及VEGF表达与肿瘤包膜完整、血管侵犯及肿瘤分化程度明显有关(P＜0.05);Flt -1与肿瘤分化程度明显有关(P＜0.05).CyclinD1与VEGF间、CyclinD1与Flt-1间及VEGF与Flt-1间的蛋白表达均呈正相关(R=0.445,P＜0.01、R=0.368,P＜0.01、R=0.332,P＜0.01).CyclinD1、VEGF及Flt-1蛋白的过表达可促使肝癌细胞增殖,使肝癌细胞具有更强的侵袭力,与肝癌的发生、发展密切相关.     

PDGF-BB、Podoplanin在非小细胞肺癌中的表达与淋巴管生成的关系

为探讨NSCLC中PDGF-BB、Podoplanin的表达与淋巴管生成的关系,用免疫组化法,检测80例NSCLC和20例癌旁正常肺组织中PDGF-BB、Podoplanin的表达;计数LMVD,结合临床病理资料统计分析.结果PDGF-BB、Podo-planin"在NSCLC中存在高表达(P<0.05);阳性表达组LMVD较阴性表达组高(P均<0.05).因此,PDGF-BB、Podo-planin在NSCLC癌细胞上存在高表达,并且与肿瘤淋巴管的生成密切相关.     

TTF-1、P40、P63在小标本非小细胞肺癌诊断中的应用及临床价值

为评价免疫组织化学染色(IHC)检测在临床中的应用价值,本研究比较了166例先后经形态学诊断及IHC检测诊断为非小细胞肺癌(NSCLC)患者的病理结果,探索两种诊断方法的一致性。并以大体病理结果作为金标准,分别比较形态学诊断、IHC结果与金标准的一致性,研究TTF-1、P40、P63与NSCLC分型的关系。结果显示,两种诊断方法在小标本NSCLC的病理分型上差异有统计学意义(P0.05),且IHC检测与金标准结果的一致性较形态学更优,并在诊断的敏感性、特异性与符合率上高于形态学。在抗原表达方面:肺腺癌中TTF-1表达的敏感性为90.5%,特异性为95.7%。肺鳞癌中P40、P63表达的敏感性分别为94.2%,89.9%;特异性为94.6%,78.4%。二者在肺鳞癌中表达的特异性差异有统计学意义(P0.05)。因此认为IHC较形态学更有助于小标本NSCLC的诊断分型。TTF-1作为指导肺腺癌诊断的指标具有重要价值。在辅助诊断肺鳞癌方面,P40较P63对鳞癌和非鳞癌的鉴别诊断更有帮助。     

胰岛素样生长因子结合蛋白3mRNA在肾癌中的表达

为探讨胰岛素样生长因子结合蛋白3(IGFBP-3)mRNA在肾癌(RCC)中的表达,采用半定量RT-PCR方法进行检测,检测56例RCC组织标本及56例癌旁组织标本中IGFBP-3的表达.结果显示RCC与癌旁组织之间IGFBP-3表达差异有显著性(P＜0.05),IGFBP-3在透明细胞癌的强阳性表达,高于其他病理类型(P均＜0.05),并与病理分级相关.因此,IGFBP-3在RCC组织中的表达具有显著性,其在肾透明细胞癌中的强阳性表达具有特异性.     

GIRK1在宫颈癌组织细胞中的表达

探求内向整流型钾离子通道1（GIRK1）与宫颈癌发生发展的关系.利用RT-PCR及免疫组织化学技术检测GIRK1 mRNA及蛋白在15例正常宫颈和30例宫颈癌组织细胞中的表达.GIRK1 mRNA在宫颈癌组织中的表达高于在正常宫颈组织中的表达,差异有统计学意义（P〈0.01）;GIRK1蛋白在宫颈癌组织中的阳性表达率为100%,在正常宫颈组织中的阳性表达率为20%,两者差异有统计学意义（P〈0.01）.GIRK1 mRNA及蛋白在宫颈癌组织中呈高表达,可能与宫颈癌的发生及发展有关.     

PTEN、p53和BAG-1在三阴乳腺癌中的表达及意义

为了研究PTEN、p53和BAG-1在三阴乳腺癌中的表达情况及临床病理意义,并讨论三者之间的关系.采用免疫组织化学SP法检测89例三阴乳腺癌中PTEN、p53、BAG-1的表达,其阳性表达率分别为44.9％、47.2％、73.0％,三者的表达均与患者的病理组织学分级、淋巴结转移情况有关(P＜0.05).PTEN的表达与p53、BAG-1表达呈负相关;BAG-1与p53的表达呈正相关,提示三者在三阴乳腺癌中的表达存在一定关联性,临床上联合检测三者可能为TNBC预后评估、患者的个体化治疗提供一定的参考.     

PDGF-BB、Podoplanin在非小细胞肺癌中的表达与淋巴管生成的关系

为探讨NSCLC中PDGF-BB、Podoplanin的表达与淋巴管生成的关系,用免疫组化法,检测80例NSCLC和20例癌旁正常肺组织中PDGF-BB、Podoplanin的表达;计数LMVD,结合临床病理资料统计分析。结果PDGF-BB、Podo-planin在NSCLC中存在高表达（P〈0.05）;阳性表达组LM...     

弥漫性大B细胞淋巴瘤预后因素探讨及IgH基因重排检测

为了探讨CD10、bcl-6蛋白表达与弥漫性大B细胞淋巴瘤(DLBCL)预后之间的关系及IgH基因重排检测在其诊断和鉴别诊断中的价值,我们采用免疫组化法观察CD10、bcl-6两种蛋白在DLBCL中的表达,半巢式PCR方法检测IgH基因重排.结果发现CD10、bcl-6蛋白阳性表达率分别为46.9%和65.6%,bcl-6阳性组预后较好.32例DLBCL中基因重排阳性率为78.1%.结论认为CD10、bcl-6蛋白表达与DLBCL预后有一定相关性,可作为判断DLBCL预后的参考指标.IgH基因重排检测在DLBCL疑难病例的诊断和鉴别诊断中有帮助.     

MMP-3,TIMP-3在人结肠癌组织中的表达及其相关性研究

探讨基质金属蛋白酶-3(MMP-3)及抑制物组织金属蛋白酶抑制剂-3(TIMP-3)在人结肠癌组织中的表达及其意义.本文采用免疫组化的方法对86例结肠癌,40例结肠腺瘤和40例正常结肠黏膜组织中的MMP-3和TIMP-3的表达进行检测.结果MMP-3在结肠癌组织中的阳性表达率高于结肠腺瘤和正常结肠组织,差异有统计学意义(P＜0.05);TIMP-3在正常结肠组织中的阳性表达率高于结肠癌组织和结肠腺瘤,差异有统计学意义(P＜0.05).结肠癌组织中MMP-3和TIMP-3蛋白的表达与结肠癌患者性别和年龄无关(P＞0.05);与癌细胞分化程度、有无淋巴结转移、有无远处器官转移及临床分期密切相关(P＜0.05).MMP-3与TIMP-3蛋白在结肠癌中的表达呈负相关(P＜0.05).TIMP-3和MMP-3表达程度及TIMP- 3/MMP-3可为结肠癌的浸润转移提供诊断依据,并为临床治疗结肠癌的浸润转移提供新的思路和方法.     

鼻咽癌组织VEGF、TNF-α及其受体VEGFR1、P55的表达及意义

观察鼻咽癌黏膜上皮中血管内皮生长因子(VEGF)、肿瘤坏死因子α(TNF-α)及受体血管生长因子1(VEGFR1),肿瘤坏死生长因子受体蛋白55(P55)的表达情况,探讨其在鼻咽癌发病及进展中的意义.我们应用免疫组化SP法检测60例鼻咽癌和60例正常鼻黏膜上皮组织VEGF、TNF-α及VEGFR1、P55表达情况,分析其在鼻咽癌组织中的表达变化及意义.结果显示鼻咽癌黏膜上皮的VEGF、TNF-α、VEGFR1、P55表达高于正常鼻黏膜上皮.因此VEGF、TNF-α及其受体VEGFR1、P55在鼻咽癌黏膜上皮中过度表达,可能参与鼻咽癌的发病及疾病进展,对于鼻咽癌的预防及治疗具有重要意义.     

Seeing and hearing and space and space and time

A previous experiment had shown spatial location to be judged in terms of the framework provided by the modality in which stimuli were presented. In the present study, digits were either auditorally or visually presented, and in either form the three digits appeared successivly to the left, in the center, and to the right of the S. The digit which occurred temporally in the middle of the sequence was never central from the spatial viewpoint. The S was asked to indicate which digit was the middle one. Ss were blind, deaf, or normal. The deaf and two control groups saw visual displays, and the blind and their controls heard auditory displays. The former groups predominantly chose the spatially middle digit and the latter groups the temporally middle. It was concluded that modality of presentation was the trigger which switched in the coding dimension of time or space.     

Bob and Carol and Tess and Ali

Conflicting religious experiences in different traditions do not necessarily defeat the rationality of conflicting beliefs sustained by those experiences in those traditions. The circularity that protects religious beliefs from such mutual defeat is not vicious. Moreover, the lack of ‘epistemological humility’ exhibited by such believers poses no threat to world peace. In fact, a campaign for compulsory humility would itself constitute a much greater threat.     

Law and Gospel,and Youth and Family Ministry

Abstract : If “faith comes through hearing” the word of God comprised in law and gospel, how do the imperatives that characterize ‘Youth and Family Ministry’ make such hearing possible? Here, literature delineating ‘Youth and Family Ministry’ is analyzed in light of its biblical foundations. The primary lens implemented in this analysis is the law/gospel hermeneutic advocated by Martin Luther and other reformers.     

Extremes of self-esteem and narcissism and the experience and expression of anger and aggression

This study examined the level of self-esteem and narcissism as personality variables involved in the disposition to experience and express anger. Three hundred thirty-eight subjects were sampled across two higher education centres and one student teaching programme in the United Kingdom. It was reasoned that individuals with both high self-esteem and narcissism would report especially high tendencies to experience and express anger and aggression and that those with high self-esteem and low narcissism would report the lowest. These predictions were influenced by theories that emphasise the role of threats to high self-esteem in the production of aggression and violence. Results indicate that groups defined by their extreme scores on self-esteem and narcissism scales produced levels of anger expression in the predicted direction. The importance of considering extreme levels of self-esteem and narcissism (in conjunction with other factors) in an analysis of anger is discussed with reference to currently influential theories in the field. Aggr. Behav. 24:421–438, 1998. © 1998 Wiley-Liss, Inc.     

Dance and psychotherapy--possibilities and limits

From a starting point of therapeutic and prophylactic components identifiable in the dance, the authors regard the dance as deserving of greater attention than was hitherto the case in programs of physiotherapy, psychotherapy, and sociotherapy. From the existing essays at a therapy basing on body and motion, they infer basic common points, and to promote discussion, they state their attitudes in respect of the potential and rank of the dance in the concept of complex multimodal psychotherapy.     

Yin and Yang,and the Hot and the Cold

Yin and Yang are important concepts in ancient Chinese philosophy.Western scholars have become more familiar with these two concepts recently,but for a long time almost no one considered comparing them with their own tradition such as the ancient Greek philosophy,and especially with the ideas of the Hot and the Cold in Presocratic philosophy.In this paper,I make an attempt to do exactly that,and especially make a detailed comparison between the thoughts of two ancient thinkers:Laozi and Anaximander.I discuss the thought of Yin and Yang in Laozi-who was the earliest philosopher making use of the concepts Yin and Yang-to express his cosmological thought in ancient Chinese philosophy.Comparatively,I discuss the ideas of the Hot and the Cold in Anaximander,the earlier among Presocratic philosophers referring to the Hot and the Cold as fundamental concepts used to establish his cosmological system.Through this comparison,I indicate that the similarity between ancient Chinese and Western traditions is far more significant than what people are used to imagining.     

Perversion and infancy and adolescence

Abstract

Studying the works of Freud and Lacan, the authors carried out research on perversion in childhood. Their work covered the perverse polymorphism of infantile sexuality and the perception of the anatomic difference between the sexes that leads the child to create sexual theories and fantasies. The phallic mother and the fetish are viewed as imaginary solutions directly related to the Oedipus and castration complexes. In perversion, a particular position is taken in relation to the Oedipus complex: a serious flaw occurs in the symbolization of the law, and the mechanisms of denial and disavowal take form. Sexuality requires a second “logical time,” separated from infancy by the latency period, in order to confirm (in action) that a fixed position of jouissance (enjoyment) has actually been established. This can only happen in a period after adolescent enhancement. The distinction between perverse traces and a perverse structure is made, taking into account the fact that, in the case of child analysis, the analyst should free the child from the family fantasy and help him/her to build his/her own fantasy.

Campos Santoro V. et al. Perversion und Kindheit und Adoleszenz.

Im Studium der Arbeiten von Freud und Lacan haben die Autoren Forschung über Perversion in der Kindheit gemacht. Die Studien behandeln den perversen Polymorphismus der infantilen Sexualität und die Wahrnehmung des anatomischen Unterschiedes zwischen den Geschlechtern, welche das Kind dazu führt, Sexual-Theorien und Phatasien zu schaffen.

Die phallische Mutter und der Fetisch werden als imaginäre Lösungen angesehen, die sich direkt auf den Ödipus- und Kastrations-Komplex beziehen. Bei der Perversion wird eine besondere Position in Bezug auf den Ödipus angenommen; ein ernsthafter Mangel tritt bei der Symbolisierung des Rechts auf; die Mechanismen der Verneinung und der Verleugnung bilden sich. Sexualität erfordert eine zweite “logische Zeit”, von der Kindheit durch die Latenz Periode getrennt, um (in der Handlung) zu bestätigen, daß eine fixierte Position der jouissance (Vergnügung) sich tatsächlich etabliert hat. Das kann sich nur in einer Periode nach der adoleszenten Verstärkung ereignen.

Es wird die Unterscheidung zwischen perversen Spuren und einer perversen Struktur gemacht, in Rechnung stellend, daß im Fall der Kinder-Analyse der Analytiker das Kind von der Familien-Phantasie befreien sollte und ihm/ihr helfen sollte, seine/ihre Phantasie auszubilden.

Campos Santoro V. Perversión e infancia y adolescencia.

Estudiando los trabajos de Freud y Lacan, los autores han investigado la perversión en la niñez. Los estudios cubren la sexualidad infantil perversa polimorfa y la percepción de la diferencia anatómica entre los sexos, que llevan al niño a crear teorías sexuales y fantasías.

La madre fálica y el fetiche son vistos como soluciones imaginarias relacionadas directamente con el complejo de Edipo y de castración. La perversión, toma una posición particular en relación al Edipo; un fallo importante tiene lugar en la simbolización de la ley; los mecanismos de negación y repudio toman forma. La sexualidad requiere un segundo “tiempo lógico” separado de la infancia por el periodo de latencia, para confirmar (en acto) que una posición fija de jouissance (goce) se ha establecido. Esto solo puede ocurrir en un periodo posterior a la intensificación adolescente.

La distinción entre las huellas perversas y la estructura perversa se ha hecho, teniendo en cuenta que, en el caso del análisis de niños, el analista debe librar al niño de la fantasía familiar y ayudar a el/ella a construir su propia fantasía.     

神圣、凡俗与净明、忠孝

本文结合有关宗教学理论,对宋元净明道所推崇的"净明"、"忠孝"概念进行了解析.作者以为:宋元净明道视世俗性的道德实践为"神圣"的宗教修炼,实与宗教学中的凡俗可以"转换"为神圣之理论相合;而净明道徒的这种"转换",则多与其个体的"宗教体验"有关.至于元代净明道借"无极"概念来释"净明",以"净明"为回复宇宙根源之境界,则合于宗教人经验到与万物的根源、终极的本体或神圣的世界契合之感.换言之,宋元净明道的"净明"、"忠孝"说,实与各种宗教的本质有着相通之处.