A device for concurrent bilateral measurement of outer eyelid closures in the rabbit

This paper describes a transducer that allows for the concurrent recording of outer eyelid closure on both the left and the right eyes of a rabbit. The device makes use of voltage induced across a current-carrying conductor when the conductor is exposed to a magnetic field perpendicular to its axis (the Hall effect). The transducer is relatively compact, inexpensive, and allows the animal to be loosely restrained.     

31例眼睑恶性肿瘤切除后Ⅰ期修复的总结

眼睑恶性肿瘤切除术后,眼睑修复时机的选择,对保持眼睑功能和美容的效果起着十分重要的作用,术式的选择是决定手术成功关键。采用眼睑全层缺损行异体巩膜片移植,替代睑板、滑行球结膜瓣前徙替代睑结膜的眼睑再造术,对31例眼睑恶性肿瘤切除,同时进行眼睑再造术。术后无排斥反应,眼睑闭合良好,外形满意。结论认为,较大范围的眼睑恶性肿瘤切除的患者,采用异体巩膜片移植、滑行结膜瓣前徙眼睑再造术能达到保持眼睑功能和美容的效果,是理想的眼睑恶性肿瘤切除后Ⅰ期修复临床决策。     

Cognitive factors in the concurrent differential conditioning of eyelid and skin conductance responses

The objectives of the study were to (1) determine if differential conditioning of eyelid responses ocurs only among subjects who accurately report knowledge of the CS-UCS relations, (2) assess whether differentially conditioned eyelid responding occurs only after the initial accurate interrial report, and (3) explore characteristics of differentially conditioned skin conductance responses (SCRs) obtained in an eyelid conditioning paradigm, with special attention to whether eyelid and SCR conditioning are similarly related to the subjects’ knowledge of the stimulus relations. Fifty-one male subjects were administered 80 differential eyelid conditioning trials, the CSs consisting of the 1,200-msec illumination of slides containing either grammatically correct or grammatically incorrect phrases. Significant differential SCR and eyelid conditioning was obtained for both V- and C-form eyelid responders, but only for subjects who accurately reported the CS-UCS relations. The initial appearance of SCR and eyelid differentiation was related within the trial sequence to subjects’ recognition of the stimulus relations. Eyelid and SCR conditioning were related similarly to knowledge of the stimulus relations, the exception being that subjects who recognized the stimulus relations late in the trial sequence did not develop reliable eyelid differentiation.     

上睑凹陷临床治疗方法的进展述评

上睑凹陷指的是上睑区眶下缘处出现的凹陷,常常与眼睑皮肤松弛下垂同时发生,为上睑老化的临床表现之一。随着人们对眶区年轻化的日渐重视,整形美容医师对纠正上睑凹陷研究出多种治疗方法。我们搜集了关于上睑凹陷临床治疗方法的国内外文献,并综合比较,分析进展趋势。目前的国内外研究中可将治疗方法概括分为三大类：组织的重新定位;注射及填充;以及两种方法的联合应用。而两种方法的联合应用正逐渐成为当今临床的主要治疗趋势。     

Some properties of conditioned and unconditioned eyelid reflexes in the albino rat

Eyelid responses in the rat were recorded as electromyographic activity (EMG) or as movements detected by a photoelectric technique. Spontaneous blinks, startle responses to acoustic stimuli, unconditioned responses to air puffs, and conditioned responses based on the corneal reflex were recorded under a variety of conditions. Special attention was given to topographical and temporal characteristics of the responses. Some relationships between the EMG and eyelid movements are described, as well as changes in eyelid responses related to habituation, conditioning, and various stimulus manipulations, In addition to the substantive findings, the study suggests that the rat may yet become a useful S in the study of eyelid conditioning.     

Simultaneous classical conditioning of two effector systems

The experiment reported here was an attempt to develop an experimental procedure for independent classical conditioning of two different response systems, the skin conductance response (SCR) and the eyelid response. In a differential conditioning design with human subjects (N = 50) two different unconditioned stimuli were used, a white noise (US1) and airpuff (US2). Two conditioned stimuli (CS11 and CS12) were paired with US1, and two other CSs (CS21 and CS22) were paired with US2. A special trial architecture was used to accomplish measurement of both anticipatory SCR and eyelid responses during each trial. Significant eyelid differentiation as well as significant SCR (especially second interval responses) differentiation developed.     

Eyelid conditioning and concomitant GSR activity

The purpose of these experiments was to investigate an aspect of autonomic activity (namely, skin resistance) during the course of eyelid conditioning, as a check on those theories which postulate that ‘arousal’ or ‘emotionality’, as measured by autonomic activity, is related to the level of conditioning.

Although many measures of skin resistance were obtained (including both basal levels and responses), the results were with one exception entirely negative in showing no significant correlations between the autonomic variables and eyelid conditioning. The exception was a significant correlation between skin resistance change to a test-trial puff of air (the UCS) and the level of eyelid conditioning reached by the subject; this result supports predictions made by Spence about the role of a hypothetical emotional response in conditioning, but was obtained in only one of the two studies.

The generally non-significant relationship between skin resistance and eyelid conditioning is discussed with respect to response specificity, and also to a postulated specificity in conditioning such that a subject who conditions well in one modality does not necessarily condition well in another modality. This specificity is considered in the light of the physiological mechanisms involved in each response system.     

Simultaneous classical conditioning of two effector systems

The experiment reported here was an attempt to develop an experimental procedure for independent classical conditioning of two different response systems, the skin conductance response (SCR) and the eyelid response. In a differential conditioning design with human subjects (N=50) two different unconditioned stimuli were used, a white noise (US₁) and an airpuff (US₂). Two conditioned stimuli (CS₁₁ and CS₁₂) were paired with US₁, and two other CSs (CS₂₁ and CS₂₂) were paired with US₂. A special trial architecture was used to accomplish measurement of both anticipatory SCR and eyelid responses during each trial. Significant eyelid differentiation as well as significant SCR (especially second interval responses) differentiation developed.     

Access to environmental stimulation via eyelid responses for persons with acquired brain injury and multiple disabilities: a new microswitch arrangement

This study assessed a new microswitch arrangement for eyelid responses using an optic sensor placed above the cheekbone and a small sticker on the person's eyelid. This new arrangement, which was designed to avoid interference of the microswitch with the person's visual functioning, was tested on three adults with acquired brain injury and multiple (consciousness, communication, and motor) disabilities. The study was carried out according to a non-concurrent multiple baseline design across participants. Data showed the new microswitch arrangement was suitable for all three participants, who increased their responding during the intervention phase of the study when their responses allowed them to access preferred stimulation. Practical implications of the findings are discussed.     

Classical discrimination conditioning of the rabbit's eyelid response using pontine stimulation as a conditioned stimulus

Classical discrimination conditioning of the nictitating membrane/eyelid response was performed on seven rabbits using stimulation of the pontine nuclei or middle cerebellar peduncle as the conditioned stimulus (CS) and an air puff as the unconditioned stimulus (US). The rabbits learned to discriminate between a CS paired with a US and delivered to one pontine nucleus (the CS+) and a CS presented alone and delivered to the contralateral pontine nucleus (the CS-). Subsequent reversal of the discrimination was also achieved when the CS+ and CS- stimulation sites were interchanged. The results are interpreted as support for the idea that essential plasticity for classical eyelid conditioning occurs efferent to the pontine nuclei, possibly in regions of the cerebellum.     

Unconditional stimulus characteristics in rabbit eyelid conditioning.

Three experiments employed aversive or positive subcortical electrical stimulation in an unconditional stimulus (US) role in rabbit eyelid conditioning. Conditional response (CR) development to a tonal conditional stimulus (CS) was examined when the intracranial stimulation was combined with a conventional cheek-shock US (Experiment 1) or with a lightflash US (Experiment 2). Our findings were consistent with previus observations that the affective impact of the US is important in the development of an overt motor CR. The complex pattern of our results, however, implies that intracranial stimulation has multiple effects on conditioning and argues against any unidimensional interpretation of these outcomes. Our analysis of CR-contingent intracranial stimulation presentation in Experiment 3 indicated that such stimulation does not act as a Thorndikian reward or punishment in rabbit eyelid conditioning.     

Blocking observed in human eyelid conditioning

Four eyelid conditioning experiments designed to be comparable to rabbit nictitating membrane (NMR) studies examined the blocking phenomenon in humans. All experiments utilized a within-subjects design, with Stage 1 of discrimination, Stage 2 of compound training, and a final test stage comparing responding to the blocked and non-blocked CSs. In two of the experiments (1 and 4) the comparison was made within subjects over all extinction trials. In Experiment 3 the test phase consisted of further reinforced training of the blocked and non-blocked CSs. These three experiments produced evidence of blocking when all extinction trials were entered into the analysis. Experiment 2, which involved a between-subjects comparison, failed to demonstrate the blocking effect. Wide variability both between and within subjects obscured the experimental effects. Post-experimental questionnaires designed to assess awareness of stimulus relations failed to identify a subjective blocking effect and showed no relationship to conditioned eyelid responding     

Neuroscience of Pavlovian conditioning: a brief review

Current knowledge on the neuronal substrates of Pavlovian conditioning in animals and man is briefly reviewed. First, work on conditioning in aplysia, that has showed amplified pre-synaptic facilitation as the basic mechanism of associative learning, is summarized. Then, two exemplars of associative learning in vertebrates, fear conditioning in rodents and eyelid conditioning in rabbits, are described and research into its neuronal substrates discussed. Research showing the role of the amygdala in fear conditioning and of the cerebellum in eyelid conditioning is reviewed, both at the circuit and cellular plasticity levels. Special attention is given to the parallelism suggested by this research between the neuronal mechanisms of conditioning and the principles of formal learning theory. Finally, recent evidence showing a similar role of the amygdala and of the cerebellum in human Pavlovian conditioning is discussed.     

Orienting to eye gaze and face processing

The author conducted 7 experiments to examine possible interactions between orienting to eye gaze and specific forms of face processing. Participants classified a letter following either an upright or inverted face with averted, uninformative eye gaze. Eye gaze orienting effects were recorded for upright and inverted faces, irrespective of whether the faces were simple, schematic faces or more realistic faces. In contrast, inversion affected orienting to targets appearing along the vertical axis. Switching the contrast between the iris and sclera reversed orienting to eye gaze. Lifting the eyelid to expose more of the iris-sclera contrast led to a potentiation of orienting to eye gaze. Raising the eyebrow alone without the eyelid did not affect orienting. The findings suggest that local perceptual information is critical for orienting to eye gaze and that the effect can occur with a degree of independence from certain types of face processing.     

Evaluation of informational control and preparatory response factors in classical aversive conditioning

Subjects performed in a differential eyelid conditioning paradigm with either airpuff or infraorbital shock as the UCS. The trial series included interpolated UCS-alone presentations, and subjects rated UCS aversiveness on all trials. Ratings of the airpuff, but not the shock UCS, were negatively correlated with the magnitude of anticipatory eyelid CRs, as predicted by preparatory response or law-of-effect models of classical conditioning. However, subjects showed no tendency to rate signaled (CS-UCS) trials as less aversive than unsignaled (UCS-alone) trials, and showed no significant preference for the signaled ucs. These results suggest that the operation of informational control and preparatory response factors is more complex than is assumed by available theories. Also, instrumental shaping and preparatory response mechanisms may not be involved in the acquisition of CRs.     

The effect of moving and static trans-scleral illumination of visual afterimages

The inside of the eye was illuminated by shining a small light through the upper eyelid and the sclera. After viewing a bright light flash, Ss closed their eyes and reported the duration of the afterimage under the following conditions: the light on and moving across the S’s eyelid (moving trans-scleral illumination), the light on but stationary (static trans-scleral illumination), and two corresponding control conditions in which the light was turned off (darkness). Results indicate that when the trans-scleral light is moving, afterimages are seen clearly for periods of approximately the same duration as those seen in complete darkness. However, when the trans-scleral illumination is stationary, the duration of the afterimages is significantly reduced. These results are compatible with other afterimage data that suggest that changes within the field after initial stimulation are needed to prolong afterimages.     

Classical conditioning of the eyeblink response in the delay paradigm in adults aged 18-83 years

To determine if age differences in classical conditioning of the eyelid response begin to appear in middle age in humans as they do in animals, adult subjects aged 18-83 years were trained in the delay conditioning paradigm. Large age effects occurred. Statistically significant differences first appeared in the decade of the 40s. Within-age-group variability was large. To reduce variability, subjects were classified by the magnitude of their unconditioned response (UR). Regardless of age, subjects with low amplitude URs conditioned poorly. In the normal UR amplitude group, the correlation between age and total percentage conditioned responses (CRs) was -.58. Eyeblink rate and voluntary responding did not account for age differences in conditioning, and it was unlikely that hearing acuity or corneal sensitivity caused the differences. Parallels between human and animal eyelid conditioning are considered, and it is suggested that age changes in the cerebellum may affect conditioning in aging mammals, including humans.     

Stimulus representations in human Pavlovian conditioning: Implications of missing negative transfer across response systems

Three Pavlovian conditioning experiments with human participants are reported, which investigated whether common or separate stimulus representations are involved in solving nonlinear discrimination tasks in different response systems. In our experiments we made use of a negative transfer effect between positive and negative patterning. Experiment 1 specified the conditions under which such a negative transfer effect occurs in human eyelid conditioning. Experiments 2 and 3 investigated whether a similar effect also occurs if two response systems- the eyelid and the skin conductance response system- are trained with trials of both types being randomly interleaved. The presence or absence of a negative transfer effect indicates whether or not the stimulus representations involved in the two conditioning processes overlap. The findings are discussed within the framework of a neuropsychological model of hippocampal function. The results suggest that the representations are distinct and thus support the idea of acquired equivalence and distinctiveness of stimulus representations.     

Accuracy of observational data with whole interval,partial interval,and momentary time-sampling recording techniques

The purpose of the study was to compare the accuracies obtained from whole interval, partial interval, and momentary time-sample recording procedures. Two types of accuracies were defined. Between-methods accuracy is the degree to which an observer using a particular recording method agrees with a standard using a continuous recording method. Within-methods accuracy is the degree to which an observer and a standard agree when both use the same recording method. Fifty-four undergraduate students viewed a videotape of a woman twisting her hair and recorded the occurrence or nonoccurrence of the behavior using one of the three recording methods. Tapes were divided into segments with low, intermediate, and high rates of behavior. Partial and whole interval recording obtained poorer between-methods accuracies than momentary time sampling. Results showed that whole interval and momentary time-sampling procedures yielded higher within-method accuracies than partial interval recording. Thus, momentary time-sampling recording provided the advantages of both greater representativeness and as few or fewer observer errors than whole or partial interval recording procedures. Use of the interval recording methods by researchers must be justifiable within this framework.     

A novel blink detection method based on pupillometry noise

Pupillometry (or the measurement of pupil size) is commonly used as an index of cognitive load and arousal. Pupil size data are recorded using eyetracking devices that provide an output containing pupil size at various points in time. During blinks the eyetracking device loses track of the pupil, resulting in missing values in the output file. The missing-sample time window is preceded and followed by a sharp change in the recorded pupil size, due to the opening and closing of the eyelids. This eyelid signal can create artificial effects if it is not removed from the data. Thus, accurate detection of the onset and the offset of blinks is necessary for pupil size analysis. Although there are several approaches to detecting and removing blinks from the data, most of these approaches do not remove the eyelid signal or can result in a relatively large amount of data loss. The present work suggests a novel blink detection algorithm based on the fluctuations that characterize pupil data. These fluctuations (“noise”) result from measurement error produced by the eyetracker device. Our algorithm finds the onset and offset of the blinks on the basis of this fluctuation pattern and its distinctiveness from the eyelid signal. By comparing our algorithm to three other common blink detection methods and to results from two independent human raters, we demonstrate the effectiveness of our algorithm in detecting blink onset and offset. The algorithm’s code and example files for processing multiple eye blinks are freely available for download (https://osf.io/jyz43).