An automated swim alley for small animals: II. Training and procedures

Experiments were conducted to establish training procedures and testing conditions that produce uniform and reliable swimming performance of rats in an automated swim alley. Data are given on the effect of water temperature and of platform rest interval on swimming speeds. In this system, rats will swim intermittently for as long as 24 h for a distance of more than 7 km.     

A fully automated two-way locomotor training apparatus

A fully automated two-way locomotor training apparatus is described. The device employs two bilevel chambers that are separated by a length of straight alley. The rat is dropped from the distinctively cued upper level of one chamber onto the grid floor of its lower level. To escape shock, the rat must leave this lower level, traverse the alley segment, and jump into the upper level of the opposite compartment. Although the animal is required to traverse the same alley in two directions, its terminal response always carries it into a distinctive goal region where shock never occurs. A brief experiment is described that was designed to assess the effectiveness of this device in the acquisition and extinction of a locomotor escape response with rats.     

Protective effect of practice on cognition during aging: implications for predictive characteristics of performance and efficacy of practice

In the present study, the effect of previous experience on spatial memory, which required the retention of information either over long intervals or within a single session, was longitudinally tested in the water maze in male F-344 rats that were from 6 to 24 months of age. Performance in these tasks was found to be age-dependent (Markowska, 1999). Other behavioral tasks in the straight alley and with a visible platform in the water maze controlled the noncognitive components of performance. For all tasks, performance was significantly correlated between 12-month-old and 18-month-old rats, indicating that cognitive performance at the early, but not advanced, stage of aging could be predicted from performance at a younger age if the novelty of the first exposure to the task was eliminated. The protective effect of experience was more robust in the reference memory task as compared to the working memory task and was modified by age when training was initiated. Behavior during the probe trials was more sensitive to the effect of aging and more resistant to the beneficial effect of practice as compared to the performance in the platform trials. The speed of swimming of experienced rats progressively decreased with age only when tested in the cognitive tasks but not in the straight alley. This indicates that speed of swimming during cognitive tasks does not exclusively reflect the ability to swim, but might be also affected by the cognitive demands of the task. Protective effect of experience on cognition was not modified by restriction in diet.     

Hypothalamic and hippocampal release of serotonin in rats bred for hyper- or hypo-anxiety

Microdialysis for measurement of serotonin in the paraventricular nucleus of the hypothalamus (PVN) and the dorsal hippocampus was performed under both basal and stimulated conditions, known to elicit differential behavioral and neuroendocrine responses in rats with inborn high (HAB) or low (LAB) anxiety-related behavior. We studied the release of hypothalamic and hippocampal serotonin in response to elevated platform exposure and forced swim stress, a mild emotional and a combined emotional and physical stressor, respectively. The data suggest that serotonin release patterns may depend on the inborn level of anxiety, the brain area dialyzed, and the stressor the animals were exposed to. Under basal conditions, no differences in serotonin release in either the PVN or dorsal hippocampus were observed between HAB and LAB rats. While in the PVN open platform exposure failed to change the release of serotonin, forced swim stress induced an increase in both HAB (p = 0.0001) and LAB (p = 0.01) rats with a significantly greater effect in the former (p = 0.027). In the dorsal hippocampus, only LABs, but not HABs, responded to the elevated platform exposure by enhancing the release of serotonin (p = 0.01). Also, forced swim stress increased hippocampal serotonin only in LAB (p = 0.002), but not HAB, rats probably indicating an involvement of hippocampal serotonin in locomotion and active stress coping. It remains to be shown to what extent the differences in serotonin release contribute to neuroendocrine and behavioral differences between HAB and LAB rats.     

Effect of Platform Used for Data Collection on Open-Ended Response Quality

Although the validity of collecting quantitative data using electronic platforms has been widely accepted, there has been concern that qualitative data collected on an electronic platform may not provide the same depth and richness in response quality as data collected using the pen-and-paper format. This article compares the quality of responses to open-ended questions collected using the pen-and-paper platform with responses collected using an electronic platform. Data for a program evaluation study were collected using either the pen-and-paper platform, an iPad, or an iPad Mini at a series of after-hours events for adults at the Center of Science and Industry, a Midwest science center. Metrics were designed to calculate a comprehensive response score consisting of the breadth of information and the richness of detail in open-ended item responses, allowing us to compare the overall response quality between the three platforms. Results indicate that qualitative data collected using electronic devices are as broad and detailed as data collected using traditional pen-and-paper techniques.     

Allocentric spatial learning by hippocampectomised rats: A further test of the “spatial mapping” and “working memory” theories of hippocampal function

This paper reports a series of three experiments that tested the “spatial-mapping” and “working-memory” theories of hippocampal function. The experimental designs incorporate separate reference- and working-memory procedures of a water-escape task, using both spatial and non-spatial learning. In Experiment 1 (Reference memory), rats with hippocampal (HC) or cortical (CC) lesions and unoperated (UNOP) rats learned to swim to a rigid visible escape platform while avoiding contact with a floating one. In the nonspatial task, the platforms each occupied any of 8 possible positions in the pool over successive trials but differed in appearance. In the spatial task, the platforms were of identical appearance but the safe one always occupied a single fixed location. The HC rats showed a highly specific spatial learning impairment but did learn to perform consistently above chance towards the end of training. In Experiment 2 (working memory), new groups of rats were trained on similar spatial and nonspatial tasks, but the platform designated correct-in terms of its visual appearance or its spatial location-was randomly changed each day. No animal learned the nonspatial task despite extensive training. Performance on the spatial version unexpectedly revealed an impairment in the CC as well as the HC group relative to the UNOP rats. However, the HCs again performed at above chance levels and demonstrated rapid (I-trial) spatial learning towards the end training. Experiment 3 used a place navigation matching-to-sample task examine spatial working memory further. Each day, an underwater platform was hidden at any of 4 possible locations, and the rats were given 2 trials to search for it. Both UNOP and CC rats located the platform faster on Trial 2 than on Trial 1, even when the inter-trial interval was long as 30min. HC rats were no faster on Trial 2 than on Trial 1. We conclude that hippocampal lesions (1) severely but partially impair spatial but not visual reference memory and (2) give rise to different patterns impairment in different working-mermory tasks. The results are a chal lenge to both the spatial-mapping and working-memory theories.     

Visual alleys as a function of instructions under informative and reduced conditions of viewing

Three experiments were designed to explore parallel and distance alleys as a function of instructions (apparent and objective) under three different distance-cue conditions. The main findings were that (1) the alleys for the apparent instructions were positioned closer to the median plane than those for the objective instructions, (2) in an indoor setting, the parallel and distance alleys were not different under either the apparent or the objective instructions, and (3) in an outdoor setting, the parallel alley lay inside the distance alley under both the apparent and the objective instructions. On the basis of comparison with some of the previous alley studies, it is suggested that exact control of instructions will not produce great discrepancy between the parallel and the distance alleys that were constructed indoors.     

Quantification of swim patterns in the morris water maze

Spatial learning and memory in rodents is most often assessed in the Morris water maze. Neurobiologists have to distinguish behavioral patterns to unravel underlying neuronal systems. We analyzed swim patterns of mice videotaped before and after training with a multitrial procedure in the water maze. In addition to traditional parameters, the animals’ position in relation to trained and other possible platform locations was estimated five times per second by an image analysis system. This parameter,cumulative distance to platform, was correlated with time spent in the platform quadrant but not with latency to and crossings of the platform location. We detected a subgroup of animals with concentric patterns within the group of spatial/persistent patterns. Random patterns were classified as well. Swim patterns before training were not predictive for the one after training. In summary, image analysis systems have made it very convenient to quantify behavior. Using their capacity, we have further improved the analysis of swim patterns, revealing animals’ different approaches to solve a problem.     

Single landmark learning in rats: Sex differences in a navigation task

In Experiments 1 and 2, rats were trained in a Morris pool to find a hidden platform located some distance away from a single landmark. Males learned to swim to the platform faster than females, but on test trials without the platform, males, unlike females, spent less time in the platform quadrant of the pool in the second half of each test trial than in the first. They also showed greater persistence in searching in the platform quadrant over a series of extinction trials. In Experiments 3a and 3b, the problem was made easier by locating the platform closer to the solitary landmark. Now males and females learned to swim to the platform equally rapidly, and both stopped searching in the platform quadrant in the second half of each test trial. Experiment 4 ruled out the possibility that males´ shorter latencies to find the platform in Experiment 2 were due to their swimming faster than females.     

Exploring multi-homing behavior of ride-sourcing drivers via real-world multiple platforms data

Multi-homing behavior refers to the behavior that ride-sourcing drivers simultaneously register and sequentially provide services on multiple ride-sourcing platforms. The multi-homing behavior of ride-sourcing drivers significantly impacts the competition among multiple ride-sourcing platforms in a competitive market. To better understand the multi-homing behavior, we present exploratory evidence on the factors that influence drivers' platform switching behavior. The RF-MNL (random forest multinomial logistic regression) framework is applied to analyze multi-homing driver behavior in a competitive ride-sourcing market. Multinomial logistic regression (MLR) is adopted to model the platform switching behavior of multi-homing drivers. The random forest is employed to seek the best combination of variables for the MLR model, which is calibrated by using the one-month multi-platform ride-sourcing data in Hangzhou, China. A variety of explanatory variables that influence ride-sourcing drivers' multi-homing behavior are estimated. The results show that the driver's socio-demographic characteristics, income level, bonus income (e.g., long-distance price rise), and work time related factors (e.g., the time gap of order dispatching, and wait time) play an essential role in determining the platform switching decision. This study corroborates the evidence of significant factors that impact drivers' switching from one ride-sourcing platform to another, which can support decision-making for ride-sourcing platforms to attract drivers serving the platform exclusively. We also examine how heterogeneity in drivers' multi-homing tendencies affects the platform's policy. To our best knowledge, this paper is one of the first quantitative studies that empirically reveal the commonly observed multi-homing behavior of ride-sourcing drivers by exploring real-world city-wide data collected on multiple platforms.     

Distance perception across spatial discontinuities

We investigated the use of nested contact relations in perceiving the relative distance of locations on discontinuous surfaces. Observers viewed computer-generated displays under monocular static conditions and adjusted a marker to match the perceived distance of a cube. The marker and cube were raised above the ground by two different platforms separated by a gap. The relative heights and distances of the platforms were varied. We found the following: (1) When spatially discontinuous surfaces are coplanar, locations of objects resting on these surfaces appear to be compared directly, bypassing relations with the underlying ground plane. (2) Spatial displacement between the platforms produces a bias, in the direction of the displacement, in the perceived relative locations of objects resting on the platforms. This suggests that local spatial relations between objects and their platforms are only partially integrated with more global spatial relations between the discontinuous surfaces of the platforms.     

Spatial memory in the rat on a hierarchical maze

Rats were trained and tested on a hierarchical radial maze, which consisted of eight primary alleys radiating from a central platform and three secondary alleys which branched off the end of each primary alley. In four experiments, rats in groups 1, 2, and 3 were tested on maze configurations consisting of one, two, or three secondary alleys, respectively, at the end of each primary alley. In Experiment 1, each group was trained to collect food pellets in the secondary alleys. By the end of training, rats in each group collected all pellets efficiently, with little repetition of entrances into either primary or secondary alleys. In Experiments 2 and 3, tests were carried out which required retention of entrances into secondary alleys, as well as primary alleys. Two trials were run in succession, with selected secondary alleys blocked on trial 1 but open and baited with food on trial 2. Animals in groups 2 and 3 showed very accurate retention of blocked secondary alleys, regardless of variation in pattern and number of alleys blocked. A fourth experiment controlled for the possible use of food cues in Experiments 2 and 3 by rebaiting all secondary alleys between trials 1 and 2. Subjects continued to choose previously blocked alleys on trial 2, thus demonstrating that choice of blocked alleys was based on memory and not on a tendency to approach visual or olfactory food cues. Several findings of these experiments suggest that memories for primary and secondary alley choices are encoded and stored within separate memory systems. Possible coding mechanisms for these systems are discussed with reference to a process of cognitive mapping.     

Control of instrumental behavior by deprivation stimuli

In Experiment 1, rats were given one trial per day in a straight alley under food deprivation on half of the trials and under water deprivation on the other half. Wet mash was available in the goal box under food deprivation for Group H and under water deprivation for Group T, the other deprivation being nonrewarded for each group. After 15--18 trials both groups ran significantly faster on their rewarded than on their nonrewarded deprivation days. A third group showed that random variation of alley color retarded formation of the discrimination. A fourth group was run in a conditional discrimination in which under food deprivation wet mash was available in a black alley, nonreward in a white alley, and vice versa under water deprivation. This group took 114 trials to begin running significantly faster in their rewarded than in their nonrewarded alley under each deprivation. In Experiment 2, it was shown that prior learning about deprivation cues "blocks" learning about alley color when alley color is subsequently presented in compound with the deprivation cue but that when both alley color and deprivation cues are relevant from the start of training, the rat learns about both cues. It is suggested that previous studies have underestimated the importance of deprivation cues by using conditional discrimination designs, choice measures rather than speeds, and parameters that are not optimal for discrimination learning.     

A computer simulation model of rats’ place navigation in the Morris water maze

Rats rapidly learn to swim from a variety of starting locations, including novel ones, to a small invisible platform submerged in a pool of cool opaque liquid. A computer simulation based on a simple perceptual memory-matching model successfully mimics this ability. The model assumes that the rat, when it successfully reaches the platform, notes the distance to prominent extramaze landmarks and stores this perceptual information in memory. When placed in the pool on subsequent trials, the simulated rat attempts to match perceived distance between itself and the landmarks to the remembered distances from the platform to the landmark. The model accounts for many of the known facts about rats’ behavior in the swimming pool and makes some interesting predictions that could be easily tested experimentally. The model has the advantage, relative to other cognitive map models, of specifying how spatial information is represented in memory and how this information guides behavior.     

Rats' sensitivity to their direction of movement and spontaneous alternation behaviour

The response of rats to their prior direction of movement was examined in three spontaneous alternation experiments. In the first, a straight alley run interpolated between two T-maze trials increased goal arm alternation when it was in the same direction as that of the animal's first T-maze goal arm entry and decreased the alternation tendency when it was in the opposite direction. Experiment II found that multiple runs in one direction in a straight alley increased the likelihood that rats would subsequently enter the goal arm of a T-maze which permitted movement in an opposite direction. In the third experiment, rats entered those goal arms of an eight-arm radial maze which permitted movement in any direction which was different from that during a preceding straight alley trial. The results of these experiments indicate that rats are quite sensitive to their direction of movement and use it as a cue in guiding their behaviour in a variety of experimental situations.     

Appropriation d'une plate-forme de coopération par des enfants en cadre scolaire

The idea of using computers in primary school to enhance or even transform learning is more and more frequent. Even if these devices are often used in an individual way, they also offer a collaborative praxis. This is possible by cooperation platforms that link the learners through informatical network. Those platforms are developed by researchers of computing in the field of CSCW (Computer-Supported Collaborative Work). It is not only a technical problem to develop those software. On the user's side (children and teachers) there is some kind of toughness for the appropriation of the technical possibilities of these tools of communication. In order to tackle these questions, it is required to work in an interdisciplinary framework. Indeed, it is possible to study the activity of the learners in interaction, mediated by digital tools, with an approach of the research in psychology (here social psychology of cognitive processes in collaborative settings). Implementing methods and categories permit the analyse of the problems of the actors to master the platform functioning. In this paper, we will describe how these analyses form the framework of the articulation between the two fields of research and how they lead to the design and the development of successive prototypes of the platform.     

Evidence of blocking with geometric cues in a virtual watermaze

Three computer based experiments, testing human participants in a non-immersive virtual watermaze task, used a blocking design to assess whether two sets of geometric cues would compete in a manner described by associative models of learning. In stage 1, participants were required to discriminate between visually distinct platforms. In stage 2, additional spatial information was provided by the shape or the color of the walls of the pool. In a test trial, the platforms were removed and the spatial knowledge acquired regarding the position of the platform was assessed. Experimental groups were compared against control groups which did not receive stage 1 training. The unique color of the correct platform, in Experiments 1 and 3, disrupted learning about the colored walls but not the geometry of the pool. In Experiment 2, the correct platform was identifiable from its position within the three platform array. Learning the relative position of the correct platform within the array disrupted learning about its position relative to the geometry of the pool, but not to the colored walls. The results suggest that learning the position of a goal in relation to the geometry of the environment can be blocked but only by an alternative geometric cue.     

Depth perception in Mongolian gerbils (Meriones unguiculatus) and spiny mice (Acomys russatus and A. cahirinus)

Depth perception in gerbils and spiny mice was studied with a modified visual cliff which varied the height of a platform from 5.08 cm to 25.4 cm and presented animals with an apparent drop-off to a patterned or a white field. Time to descend from the platform and orienting response frequency were recorded. For gerbils neither measure varied significantly between the platforms. Both Acomys species increased descent time and orienting response frequency as platform height increased. The results suggest that both gerbils and spiny mice can perceive depth, though there appears to be a difference between the species' use of sensory cues in descending from a visual cliff. The spiny mice appear to rely more on visual cues than do the gerbils. The data also reveal subtle behavioral differences between the Acomys species that may relate to their successful sympatry. The results are discussed by alluding to ecological differences between these species in their natural habitats.     

Effects of Unilateral Entorhinal Cortex Lesion on Retention of Water Maze Performance

In a previous study, adult male Sprague-Dawley rats with unilateral, electrolytic entorhinal cortex lesions showed significant deficits in acquisition of a water maze task that measured working memory. The 10 days of testing used two trials per day with an intertrial interval of 1 h, and the rats with entorhinal damage were impaired in total distance to the platform in both trials. In the present retention study, rats who learned the same task prior to injury and were then retested for 5 days after lesion showed only a first day deficit in total distance to platform in the second trial. Analysis of swim patterns indicated that rats with unilateral entorhinal lesions used an altered strategy in retention testing to find the platform in the second trial of each day and incorporated the use of headings appropriate for Trial 1 only. This altered or compensatory strategy was not the optimum choice for problem solution. Although the rats then were able to switch headings and find the platform without significant impairment in total distance to platform on days 2–5 of testing, the use of an initial incorrect strategy indicated subtle residual deficits in cue integration and use of working memory.     

Partial reinforcement (acquisition) effects within subjects

Acquisition performance of 22 rats in a straight alley runway was examined. The animals were subjected to partial reinforcement when the alley was black (B±) and continuous reinforcement when it was white (W+). The results indicated (a) higher terminal performance, for partial as against continuous reinforcement conditions, for starting-time and running-time measures, and (b) lower terminal performance under partial conditions for a goal-entry-time measure. These results confirm within subjects an effect previously demonstrated, in the runway, only in between-groups tests, where one group is run under partial reinforcement and a separate group is run under continuous reinforcement in the presence of the same external stimuli. Differences between the runway situation, employing a discrete-trial procedure and performance measures at three points in the response chain, and the Skinner box situation, used in its free-operant mode with a single performance measure, are discussed in relation to the present findings.