Protein synthesis inhibition blocks consolidation of an acrobatic motor skill

To investigate whether motor skill learning depends on de novo protein synthesis, adult rats were trained in an acrobatic locomotor task (accelerating rotarod) for 7 d. Animals were systemically injected with cycloheximide (CHX, 0.5 mg/kg, i.p.) 1 h before sessions 1 and 2 or sessions 2 and 3. Control rats received vehicle injections before sessions 1, 2, and 3. Although CHX did not affect improvement of performance within session 1, between-session improvement was impaired. In overtrained animals, comparable injections of CHX had no effect on rotarod performance. These findings suggest that consolidation of motor skills requires protein synthesis.     

Training of fast tapping with reduction of kinaesthetic, tactile, visual and auditory sensations

Five subjects were trained to tap on a light Morse-key during nerve compression block. The training sessions lasted for 40 sec., with a 5 sec. rest after the first 20 sec. work period. The group learning curve reached 89.5 per cent. level of normal performance by the eighth training session. In the ninth, the testing session, subjects tapped with visual and auditory sense reduction superimposed on the kinaesthetic and tactile impariment of the training condition. Performance in the testing session reached 40.9 per cent. of normal.

The sixth subject was trained in the same task as the other five subjects, but the training condition included elimination of cues from all four sensory channels. He reached 79.09 per cent. of his normal tapping performance in the seventh session.

These results show that the motor skill of tapping can be relearned in the absence of kinaesthetic cues. Furthermore when the subject has no conscious knowledge of any peripheral sensory cues connected with the ongoing motor activity, learning can nevertheless take place. These findings lead to the hypothesis, that skilled motor activity can be monitored by central processes alone.

During the training sessions subjects showed a tendency of tapping in groups of gradually increasing length. It is hypothesized that increased number of taps forming a group gives an indication to the possible mode of action of these central processes.     

The effect of tire grip on learning driving skill and driving style: A driving simulator study

There is a need for training methods that improve the driving skill and driving style of novice drivers. Previous research in motor learning has shown that degrading the task conditions during practice can enhance long-term retention performance. Inspired by these findings, this study investigated the effects of the tire-road friction coefficient on learning a self-paced lane-keeping task in a driving simulator. A sample of 63 young and inexperienced drivers were divided into three groups, low grip (LG), normal grip (NG) and high grip (HG), who practiced driving with a friction coefficient of 0.45, 0.90, and 1.80, respectively. All groups drove six 8 min sessions on a road with curves in a rural environment: four practice sessions, an immediate retention session, and a delayed retention session on the next day. The two retention sessions were driven with normal-grip tires. The results show that LG drove with lower speed than NG during practice and retention. Transferring from the last practice session to the immediate retention session, LG’s workload decreased, as measured with a secondary task, whereas HG’s workload increased. During the immediate retention session, LG had less road departures than HG, but HG drove closer to the lane center in curves than the other two groups. HG reported elevated confidence during practice, but not in retention. In conclusion, this simulator-based study showed that practicing with low-grip tires resulted in lower driving speeds during retention tests, an effect which persisted overnight. These results have potential implications for the way drivers are trained.     

Effect of cognitive demand during training on acquisition, retention and transfer of a postural skill

The study examined whether attentional demands of a concurrent cognitive task during balance training affect the acquisition, retention and transfer of a postural control skill. Single-leg balance was evaluated in 64 volunteers (mean age 24.0 years, SD 3.10 years) while performing either a cognitive task requiring little attention (forward counting) or a highly demanding cognitive task (arithmetic manipulation) following three days of training. Skill retention was evaluated two days following the cessation of training, and transfer was determined by changes in the untrained extremity. Three training sessions induced decreases in mean sway velocity and amplitude variability. Skill retention was enhanced in the group trained under conditions with greater attentional demands, suggesting that diverting attention away from the postural task and allowing learning to involve more automatic processes may enhance the learning of such tasks. Practice induced similar changes in the trained and untrained extremities following both training protocols.     

Simultaneous training on two hippocampus-dependent tasks facilitates acquisition of trace eyeblink conditioning

A common cellular alteration, reduced post-burst afterhyperpolarization (AHP) in CA1 neurons, is associated with acquisition of the hippocampus-dependent tasks trace eyeblink conditioning and the Morris water maze. As a similar increase in excitability is correlated with these two learning paradigms, we sought to determine the interactive behavioral effects of training animals on both tasks by using either a consecutive or simultaneous training design. In the consecutive design, animals were trained first on either the trace eyeblink conditioning task for six sessions, followed by training on the water maze task for six sessions, or vice versa. The simultaneous design consisted of six or 11 training days; animals received one session/day of both trace eyeblink conditioning and water maze training. Separate groups were used for consecutive and simultaneous training. Animals trained on both tasks simultaneously were significantly facilitated in their ability to acquire the trace eyeblink conditioning task; no effect of simultaneous training was seen on the water maze task. No effect was seen on acquisition for either task when using the consecutive training design. Taken together, these findings provide insight into how the hippocampus processes information when animals learn multiple hippocampus-dependent tasks.     

Application of Variability of Practice Hypothesis in Alzheimer Patients

This study examined the effects of constant versus variable practice on learning a simple motor task in eight patients diagnosed with Alzheimer's Disease (AD). According to the variability of practice hypothesis, variations in practice of a motor skill result in superior learning as evidenced by better ability to transfer the skill. Eight AD patients were matched for Mini-Mental State Exam and baseline scores and then randomly assigned to a constant or variable practice group. Then, the participants were trained in hitting a tennis ball with a small racquet against a horizontal target (practice task) over 12 sessions. Finally, they were asked to aim at a vertical target (transfer task). All participants showed improvement on the practice task. On the transfer task, three of four participants in the variable practice group outperformed their matched counterparts, suggesting learning benefits of variable practice.     

Task specificity and neural adaptations after balance learning in young adults

BackgroundOnly 30 min of balance skill training can significantly improve behavioral and neuromuscular outcomes. However, it is unclear if such a rapidly acquired skill is also retained and transferred to other untrained balance tasks.Research questionWhat are the effects of a single balance training session on balance skill acquisition, retention, and transferability and on measures of neural plasticity examined by transcranial magnetic brain stimulation (TMS) and inter-muscular coherence?MethodsHealthy younger adults (n = 36, age 20.9, 18 M) were randomly assigned to: Balance training (BT); Active control (cycling training, CT) or non-active control (NC) and received a 20-min intervention. Before, immediately and ~ 7 days after the interventions, we assessed performance in the trained wobble board task, untrained static standing tasks and dynamic beam walking balance tasks. Underlying neural plasticity was assessed by tibialis anterior motor evoked potential, intracortical facilitation, short-interval intracortical inhibition and long-interval intracortical inhibition using TMS and by inter-muscular coherence.ResultsBT, but not CT (18%, d = 0.32) or NC (−1%, d = −0.02), improved balance performance in the trained, wobble board task by 207% (effect size d = 2.12). BT retained the acquired skill after a 1-week no-training period (136%, d = 1.57). No changes occurred in 4 measures of balance beam walking, in 8 measures of static balance, in 8 measures of intermuscular coherence, and in 4 TMS measures of supra-spinal plasticity (all p > 0.05).SignificanceHealthy young adults can learn a specific balance skill very rapidly but one should be aware that while such improvements were retained, the magnitude of transfer (32%, d = 0.94) to other balancing skills was statistically not significant. Additional studies are needed to determine the underlying neural mechanisms of rapid balance skill acquisition, retention, and transfer.     

The effects of distributed training on retention of operant conditioning in human infants

Long-term retention of operant footkicking acquired in the mobile conjugate reinforcement paradigm was assessed as a function of the distribution of training time. In the first study, 3-month-old infants were trained for either one 18-min session or for two 9-min or three 6-min sessions separated by 24-hr intervals. All infants exhibited retention during a test administered immediately after training, but only those trained in a single session continued to perform the conditioned response during cued-recall tests 7 or 14 days later. Infants trained in three sessions showed no evidence of remembering the contingency even after a week. A warm-up decrement, seen in the day-to-day performance of infants in the distributed conditions, was eliminated in the second study by the interpolation of a nonreinforcement period at the outset of daily sessions. This procedure also enhanced long-term retention such that infants trained in three 6-min session now remembered the contingency for 14 days and did not differ from infants who had received a single 18-min session. Whether distributed training facilitates or impairs long-term retention appears to depend on the opportunity for infants to acquire a sufficient number (or kind) of effective retrieval cues during original learning.     

Flavor preferences produced by backward pairing with wheel running

In 3 experiments rats given 8 sessions of preexposure to wheel running acquired a preference for a flavor that was given immediately after each of 4 subsequent sessions of wheel running. Such flavor preference was less likely when rats were given the same conditioning procedure but without preexposure to wheels (Experiment 1) or when access to flavor was delayed by 30 min following a wheel session (Experiment 2). When rats were given a flavor before each wheel session, the resulting conditioned aversion was greater in rats that had no prior exposure to wheel running (Experiment 3). These results show that whether an aversion or preference for a flavor is produced by wheel running depends on an interaction between prior wheel experience and the sequence of events.     

Patterns of interference in sequence learning and prism adaptation inconsistent with the consolidation hypothesis

The studies reported here used an interference paradigm to determine whether a long-term consolidation process (i.e., one lasting from several hours to days) occurs in the learning of two implicit motor skills, learning of a movement sequence and learning of a visuo-motor mapping. Subjects learned one skill and were tested on that skill 48 h later. Between the learning session and test session, some subjects trained on a second skill. The amount of time between the learning of the two skills varied for different subjects. In both the learning of a movement sequence and the learning of a visuo-motor mapping, we found that remote memories were susceptible to interference, but the passage of time did not afford protection from interference. These results are inconsistent with the long-term consolidation of these motor skills. A possible difference between these tasks and those that do show long-term consolidation is that the present tasks are not dynamic motor skills.     

Effects of repetitive motor training on movement representations in adult squirrel monkeys: role of use versus learning

Current evidence indicates that repetitive motor behavior during motor learning paradigms can produce changes in representational organization in motor cortex. In a previous study, we trained adult squirrel monkeys on a repetitive motor task that required the retrieval of food pellets from a small-diameter well. It was found that training produced consistent task-related changes in movement representations in primary motor cortex (M1) in conjunction with the acquisition of a new motor skill. In the present study, we trained adult squirrel monkeys on a similar motor task that required pellet retrievals from a much larger diameter well. This large-well retrieval task was designed to produce repetitive use of a limited set of distal forelimb movements in the absence of motor skill acquisition. Motor activity levels, estimated by the total number of finger flexions performed during training, were matched between the two training groups. This experiment was intended to evaluate whether simple, repetitive motor activity alone is sufficient to produce representational plasticity in cortical motor maps. Detailed analysis of the motor behavior of the monkeys indicates that their retrieval behavior was highly successful and stereotypical throughout the training period, suggesting that no new motor skills were learned during the performance of the large-well retrieval task. Comparisons between pretraining and posttraining maps of M1 movement representations revealed no task-related changes in the cortical area devoted to individual distal forelimb movement representations. We conclude that repetitive motor activity alone does not produce functional reorganization of cortical maps. Instead, we propose that motor skill acquisition, or motor learning, is a prerequisite factor in driving representational plasticity in M1.     

Context extinction and associative learning in Lymnaea

Aerial respiratory behavior in the pond snail Lymnaea was operantly conditioned so that snails learned not to perform aerial respiration in a hypoxic environment. Snails were trained in either the standard context (no food odorant) or a carrot (food-odorant) context. An operant training procedure of two 45-min training sessions with a 1-h interval between the sessions followed by a third 45-min training session 18 h later was sufficient to produce associative learning and long-term memory (LTM) that persisted for at least 5 days. If, however, following the third operant training session snails received three 45-min extinction training sessions, with each extinction session separated by at least a 1-h interval, LTM was not observed when tested the following day. That is, the memory was extinguished. Extinction, however, did not occur if the context of the extinction training was different from the context of the associative training. That is, in the snails trained in the standard context, extinction did not occur if the extinction training sessions were performed in the food-odorant context and vice versa.     

Does exercise-induced muscle damage impair subsequent motor skill learning?

Motor skill learning is a fundamental aspect of human behavior based on the calibration of internal models via sensory information such as proprioception. Some conditions, as exercise-induced muscle damage (EIMD), disrupt proprioceptive information, and may cause learning impairment. Such possible relation between EIMD and motor skill learning has not yet been investigated and it is the aim of this study. For this purpose, thirty male university students (19.3 ± 1.8 years) were equally assigned to two groups: EIMD and CON group. The EIMD group received a treatment to induce muscle damage consisting of a weight lifting protocol directed to the agonist muscles related to the task prior to the pretest and to the learning sessions. EIMD was verified and compared between groups and along the process (0–168 h) by means of the degree of delayed onset muscle soreness (DOMS), perceived total quality recovery and maximal isometric strength (MIS). To investigate motor skill learning, both groups practiced a dart throwing task for four sessions with 150 trials in each session. Recovery status and DOMS were recovered at 96 h in the EIMD group, and MIS was not recovered throughout 168 h. In contrast, muscle damage parameters were not altered across 168 h in the CON group. Accuracy and consistency were compared within and between groups in a pretest posttest design. The EIMD group showed less accurate and consistent results on the long term (delayed posttest). Results confirmed our hypothesis that EIMD, a common condition in sports and in rehab practices, may hinder motor skill learning, possibly due to neurological aspects such as proprioceptive information, its relation to central nervous system reorganization and internal model consolidation.     

Sleep-dependent consolidation of procedural motor memories in children and adults: the pre-sleep level of performance matters

In striking contrast to adults, in children sleep following training a motor task did not induce the expected (offline) gain in motor skill performance in previous studies. Children normally perform at distinctly lower levels than adults. Moreover, evidence in adults suggests that sleep dependent offline gains in skill essentially depend on the pre-sleep level of performance. Against this background, we asked whether improving children's performance on a motor sequence learning task by extended training to levels approaching those of adults would enable sleep-associated gains in motor skill in this age group also. Children (4-6 years) and adults (18-35 years) performed on the motor sequence learning task (button-box task) before and after ~2-hour retention intervals including either sleep (midday nap) or wakefulness. Whereas one group of children and adults, respectively, received the standard amount of 10 blocks of training before retention intervals of sleep or wakefulness, a further group of children received an extended training on 30 blocks (distributed across 3 days). A further group of adults received a restricted training on only two blocks before the retention intervals. Children after standard training reached lowest performance levels, whereas in adults performance after standard training was highest. Children with extended training and adults after reduced training reached intermediate performance levels. Only at these intermediate performance levels did sleep induce significant gains in motor sequence skill, whereas performance did not benefit from sleep in the low-performing children or in the high-performing adults. Spindle counts in the post-training nap were correlated with performance gains at retrieval only in the adults benefitting from sleep. We conclude that, across age groups, sleep induces the most robust gain in motor skill at an intermediate pre-sleep performance level. In low-performing children sleep-dependent improvements in skill may be revealed only after enhancing the pre-sleep performance level by extended training.     

Practice makes transfer of motor skills imperfect

We investigated the practice-effects on motor skill transfer and the associated representational memory changes that occur during the within-practice and between-practice phases. In two experiments, participants produced extension–flexion movements with their dominant right arm for a limited or prolonged practice session arranged in either a single- or multi-session format. We tested the ability of participants to transfer the original pattern (extrinsic transformation) or the mirrored one (intrinsic transformation) to the non-dominant left arm, 10?min and 24?h after the practice sessions. Results showed that practice induces rapid motor skill improvements that are non-transferable irrespective of the amount of acquisition trials. Furthermore, the extrinsic component of the skill develops early and remains the dominant coding system during practice. Conversely, we found distinct between-practice memory changes: a limited practice induces an off-line development of the extrinsic component, whereas a prolonged practice session subserves the off-line development of the intrinsic component (Experiment 2). We provided further evidence that the long-term representation of the motor skill also depends on the nature of the practice session itself: the parsing of practice into multiple sessions narrows the effector-transfer capacities in comparison to a single session (Experiment 1). These findings yield theoretical and practical implications that are discussed in the context of recent motor skill learning models.     

Structural Stability within the Lateral Cerebellar Nucleus of the Rat Following Complex Motor Learning

Complex motor learning, but not mere motor activity, has been previously shown to induce structural modifications within the cerebellar cortex. The present experiment examined whether similar changes occur within one of the primary output targets of the region of the cerebellar cortex in which these structural changes were described, the lateral cerebellar nucleus (LCN; dentate nucleus). Adult female rats were randomly allocated to one of three training conditions. Acrobatic condition (AC) rats were trained to complete a complex motor learning task consisting of a series of elevated obstacles while motor control (MC) condition animals were forced to traverse a flat obstacle-free runway equal in length to the AC task. Inactive condition (IC) animals received no motor training. Unbiased stereological techniques and electron microscopy were used to obtain estimates of synapse number and postsynaptic density (PSD) length within the LCN. Results showed that neither synapse number nor PSD length was significantly altered as a function of training condition. These results indicate that complex motor skill learning is associated with structural plasticity within the cerebellar cortex and with structural stability within the lateral cerebellar nucleus.     

Optimal training design for procedural motor skills: a review and application to laparoscopic surgery

This literature review covers the choices to consider in training complex procedural, perceptual and motor skills. In particular, we focus on laparoscopic surgery. An overview is provided of important training factors modulating the acquisition, durability, transfer, and efficiency of trained skills. We summarize empirical studies and their theoretical background on the topic of training complex cognitive and motor skills that are pertinent to proficiency in laparoscopic surgery. The overview pertains to surgical simulation training for laparoscopy, but also to training in other demanding procedural and dexterous tasks, such as aviation, managing complex systems and sports. Evidence-based recommendations are provided for facilitating efficiency in laparoscopic motor skill training such as session spacing, adaptive training, task variability, part-task training, mental imagery and deliberate practice.     

Long-term retention of a motor skill: Implicit sequence knowledge is not retained after a one-year delay

Experimental work shows that some motor skills are retained well over a long delay and others are not. It is possible that motor tasks that are primarily implicit are retained over long intervals, whereas those with an explicit component are subject to more rapid forgetting. To test whether implicit motor skills are retained, 24 (experienced) participants were retested after a one-year delay for their retention of an implicit motor sequence. They had been trained on the sequence in a button-pushing task. Although the experienced participants responded faster than they had a year earlier, they showed no retention of the sequence compared to 24 participants who had received no training a year earlier, and no savings in relearning the sequence. The overall speed effect may indicate that the amount of training is crucial to the degree of retention of a skill. The results indicate that it is not the case that implicit motor skills are well retained whereas those with an explicit component are not.     

Gradual training reduces practice difficulty while preserving motor learning of a novel locomotor task

Motor learning strategies that increase practice difficulty and the size of movement errors are thought to facilitate motor learning. In contrast to this, gradual training minimizes movement errors and reduces practice difficulty by incrementally introducing task requirements, yet remains as effective as sudden training and its large movement errors for learning novel reaching tasks. While attractive as a locomotor rehabilitation strategy, it remains unknown whether the efficacy of gradual training extends to learning locomotor tasks and their unique requirements. The influence of gradual vs. sudden training on learning a locomotor task, asymmetric split belt treadmill walking, was examined by assessing whole body sagittal plane kinematics during 24 hour retention and transfer performance following either gradual or sudden training. Despite less difficult and less specific practice for the gradual cohort on day 1, gradual training resulted in equivalent motor learning of the novel locomotor task as sudden training when assessed by retention and transfer a day later. This suggests that large movement errors and increased practice difficulty may not be necessary for learning novel locomotor tasks. Further, gradual training may present a viable locomotor rehabilitation strategy avoiding large movement errors that could limit or impair improvements in locomotor performance.     

Role of Constant,Random and Blocked Practice in an Electromyography-Based Oral Motor Learning Task

Purpose: The role of principles of motor learning (PMLs) in speech has received much attention in the past decade. Oral motor learning, however, has not received similar consideration. This study evaluated the role of three practice conditions in an oral motor tracking task.

Method: Forty-five healthy adult participants were randomly and equally assigned to one of three practice conditions (constant, blocked, and random) and participated in an electromyography-based task. The study consisted of four sessions, at one session a day for four consecutive days. The first three days sessions included a practice phase, with immediate visual feedback, and an immediate retention phase, without visual feedback. The fourth session did not include practice, but only delayed retention testing, lasting 10–15 minutes, without visual feedback.

Results: Random group participants performed better than participants in constant and blocked practice conditions on all the four days. Constant group participants demonstrated superior learning over blocked group participants only on day 4.

Conclusion: Findings indicate that random practice facilitates oral motor learning, which is in line with limb/speech motor learning literature. Future research should systematically investigate the outcomes of random practice as a function of different oral and speech-based tasks.