情绪唤醒对工作记忆的作用

个体的情绪状态影响工作记忆。以往对情绪对工作记忆影响效应有不同的结果。这与研究者对情绪和工作记忆的操作定义有关。以往的研究关注于情绪效价而忽视了唤醒对工作记忆的影响,因此所得的结论可能产生混淆。本文综述了情绪唤醒如何影响工作记忆,以及这一过程的神经机制和脑机制。研究发现,注意在情绪唤醒对工作记忆影响中起着重要的调节作用,中度的唤醒对工作记忆有较强促进作用。     

5-羟色胺基因缺陷增强急性应激后高唤醒状态

剧烈的应激刺激会引起持续的高唤醒状态, 是多种应激障碍的核心症状, 并推进其他症状的发生发展。本研究关注5-羟色胺在应激诱发高唤醒的发生、发展中的作用, 通过测量听觉惊吓反射水平反映高唤醒状态, 考察色氨酸羟化酶-2基因缺陷小鼠在天敌或电击应激前后高唤醒的变化。研究发现, 雄性基因缺陷小鼠在应激后出现持续一周以上的高唤醒表现, 而野生型小鼠高唤醒状态很快恢复。结果提示, 基因缺陷引起的5-羟色胺降低可能是强应激诱发的持续高唤醒的易感因素。     

情绪性场景图片加工的ERP研究:成果与问题

近年来,以情绪性场景图片做为刺激进行事件相关电位(ERP)的研究越来越多。特别是情绪效价效应和唤醒效应在相关ERP成分(P1、P2、EPN、N2、P300和正性慢波)上的反映更是情绪研究的一个热点。情绪效价ERP效应的研究主要集中在对情绪负性偏向的研究上,唤醒效应反映了情绪的动机作用。然而,情绪性场景图片加工的ERP研究结果存在许多不一致,这些不一致可能源于注意资源、图片评定、图片内容、刺激物理特征、任务、个体差异等因素的影响。如何将行为结果、非情绪ERP证据、情绪ERP证据结合起来,对不同神经生理学方法和刺激类型加以整合,尚需很长的路要走。     

儿童特质推理与情绪和效价线索理解的关系

本研究从情绪、效价两个角度探讨儿童特质理解与线索理解的关系。372名4、5、7岁儿童完成了贴标签和行为预测两类特质推理任务。实验一显示,所有年龄段儿童都能完成对情绪性特质害羞和胆小的推理,且特质和行为评定与情绪评定呈显著正相关;实验二显示,所有年龄段均能完成双重性质特质助人为乐和自私的推理,特质和行为评定与效价、情绪评定均呈显著正相关,回归分析则只有效价评定进入方程。结果说明,儿童完成情绪性特质推理任务时与情绪理解能力关系密切,而对双重性质特质推理时更多的与效价理解能力有关。     

情绪性记忆的定向遗忘效应

刺激的情绪性具有增强记忆的效果,那么,带有情绪信息的刺激对遗忘又会产生怎样的影响？已有研究从行为及认知神经机制层面探讨了情绪性刺激对定向遗忘效应的影响,但结果并不一致。总结已有文献发现,以情绪性刺激的材料类型、情绪效价、唤醒度和熟悉度为主的客观因素和以被试的情绪状态为主的主观因素均为影响定向遗忘效应产生及其稳定性的重要原因。这类研究是对有意遗忘研究领域的重要补充,也加深了人们对有意遗忘机制的理解。     

不同线索下特质焦虑个体的返回抑制

焦虑与注意偏向的研究是近年来情绪与认知领域的热点。为探讨特质焦虑个体的注意偏向特点及其返回抑制能力是否受不同线索的调节, 采用特质焦虑量表筛选高特质焦虑大学生29名, 低特质焦虑大学生28名完成线索-靶子任务。要求被试在提示线索消失后, 对位置进行快而准地辨别反应, 分别探索中性和情绪性提示线索下被试的返回抑制。结果发现：(1)在中性线索条件下, 高焦虑个体平均反应时慢于低焦虑个体。(2) 在情绪线索条件下, 高焦虑个体在负性线索下的反应时小于在正性线索下的反应时; 高、低焦虑个体在各种SOA条件下均出现了返回抑制, 但各组返回抑制量受到情绪线索的调节：在正性情绪线索条件下, 两组返回抑制量没有显著差异; 在负性情绪线索下, 高焦虑个体返回抑制量显著小于低焦虑个体。这表明, (1)焦虑个体的注意偏向受到刺激信息的影响：只对负性情绪线索出现注意警觉; (2)只有在涉及负性情绪信息时高、低焦虑个体返回抑制能力才有差异, 高焦虑个体存在对负性情绪线索的抑制困难。     

成就目标定向、测验焦虑与工作记忆的关系

在工作记忆广度测验情境下,以152名中学生为被试,考察成就目标定向、测验焦虑和工作记忆的关系。结果表明四种成就目标定向与测验焦虑和工作记忆间具有不同的关系模式：成绩-接近目标与较低的测验焦虑水平和较高的工作记忆广度相关;掌握-接近目标与担忧、情绪化,与工作记忆广度相关不显著;成绩-回避目标与测验焦虑中的担忧和情绪化正相关,与工作记忆广度负相关;掌握-回避目标与担忧和情绪化存在正相关,与工作记忆广度存在接近显著的相关。测验焦虑是成绩-接近和成绩-回避目标影响工作记忆的中介变量。在测验焦虑中,情绪化对工作记忆的干扰大于担忧的干扰     

红色和蓝色对中国汉族大学生情绪的启动效应

通过3个实验探讨红色和蓝色对中国汉族大学生的情绪意义。实验1采用限制联想法考察红色和蓝色的情绪意义。实验2、实验3采用启动范式, 分别考察在知觉水平和概念水平上红色和蓝色对情绪的启动效应。结果发现：(1)知觉水平：在唤醒度和优势度上, 红色知觉引发了高唤醒、高优势情绪, 蓝色知觉引发了低唤醒、低优势情绪, 这与国外研究基本一致; 但在愉悦度上, 红色知觉既能引发高愉悦情绪, 也能引发低愉悦情绪, 而蓝色知觉只引发了高愉悦情绪, 这体现出本土化特点。(2)概念水平：在唤醒度和愉悦度上, 红色概念引发了高唤醒、高优势情绪, 蓝色概念引发了低唤醒、低优势情绪, 这与知觉水平基本一致; 但在愉悦度上, 红色概念只启动了高愉悦情绪, 而蓝色概念同时启动了高愉悦和低愉悦的情绪, 这与知觉水平不一致。本研究表明：(1)红色和蓝色对中国汉族大学生情绪引发的特点主要表现在愉悦度上; (2)颜色知觉和颜色概念对情绪的引发机制可能具有如下特点：颜色知觉对情绪的三维度具有直接的引发作用; 而颜色概念对情绪唤醒度和优势度的引发是以颜色知觉为中介, 对愉悦度的引发则是以社会文化为原因。本研究探讨了红色和蓝色对中国汉族大学生情绪的作用, 对红色和蓝色在中国社会环境中的应用具有一定的实际价值; 同时, 直接比较并剖析了颜色知觉与概念对情绪的引发特点, 对于揭示二者对情绪引发的内部机制具有一定的理论意义。     

父亲情绪表达与儿童社会适应:气质的调节作用

通过对341名学前儿童进行问卷调查和任务测查,考察了父亲情绪表达、儿童气质与儿童社会适应的关系。结果表明:(1)父亲积极情绪表达对儿童社会能力有正向预测作用;父亲消极情绪表达对儿童社会能力具有负向预测作用,而对外显、内隐行为问题有正向预测作用。(2)儿童的积极情绪性对其外显行为问题与社会能力均有显著正向预测作用;消极情绪性对其社会能力有负向预测作用,而对其外显行为问题有正向预测作用;儿童的努力控制对其外显行为问题有较强地负向预测作用,对其社会能力具有正向预测作用。(3)父亲情绪表达与儿童努力控制、消极情绪性对儿童社会适应有显著交互作用,父亲情绪表达对不同气质特点儿童的社会适应具有不同的预测作用。     

MAOA基因与环境对反社会行为的交互作用及其可能的脑机制

反社会行为具有重要的遗传学基础。MAOA基因是反社会行为的重要候选基因,该基因与环境对反社会行为具有交互作用,然而其内在作用机制尚不清楚。与情绪管理相关的脑区和神经回路,以及与工作记忆能力相关的脑区和神经回路在其中起重要作用。未来研究可从多基因-环境交互作用、理论模型验证、脑结构与功能的中介作用等方面进一步深化MAOA基因与反社会行为的关系研究。     

Fischer 344 and wistar rats differ in anxiety and habituation but not in water maze performance

The fact that various neuropharmacological substances have anxiolytic as well as amnesic effects suggests that neuronal mechanisms of anxiety and learning/memory closely interact. Hence, we hypothesized that differences in anxiety-related behavior could be accompanied with differences in cognition or habituation. Two rat strains with different levels of anxiety, more anxious Fischer 344 rats by Charles River (FC) and less anxious Wistar rats by Winkelmann (WW), were tested in the Morris water maze task and an open field test for habituation learning. Additionally, we investigated the effect of different light intensities on the performance in the Morris water maze and the elevated plus maze. The results of the water maze task indicate that differences in anxiety-related behavior do not go along with differences in this performance of learning/memory. Moreover, the test was not affected by different light intensities. In contrast, illumination did affect performance in the elevated plus maze test, wherein dim light provoked an anxiolytic effect in both rat strains. The findings that neither different baseline levels of anxiety nor fear modulating light conditions were accompanied by changes in the performance of rats in the Morris water maze led us to the suggestion that there is no connection between anxiety and learning/memory in this task. Contrarily, anxiety might be associated with habituation learning in the open field test, shown by the superior habituation of the anxious FC rats in comparison to the less anxious WW rats. In sum, these results indicate that anxiety and learning/memory seem to be independently regulated behaviors, whereas habituation might be more closely correlated with anxiety. Nevertheless, a general statement about the relation between emotionality and learning/memory mechanisms would be premature and the link between behaviors remains to be clarified.     

Search strategies used by APP transgenic mice during navigation in the Morris water maze

TgCRND8 mice represent a transgenic mouse model of Alzheimer's disease, with onset of cognitive impairment and increasing amyloid-beta plaques in their brains at 12 weeks of age. In this study, the spatial memory in 25- to 30-week-old TgCRND8 mice was analyzed in two reference and one working memory Morris water maze (MWM) tests. In reference memory tests, the mice were trained to escape to a hidden platform, which in one version of the test was marked by a visual cue. In the working memory test, the hidden platform was moved daily to different locations. The TgCRND8 mice were impaired in reference memory when trained in a hidden platform test. However, the mice developed spatial memory comparable to non-Tg littermates in a cued reference memory test. The mice showed also an impairment in spatial working memory. Analysis of search paths revealed that in contrast to non-Tg littermates, TgCRND8 mice did not use spatial strategies during their navigation. Instead, they learned to locate an escape platform using a nonspatial, chaining strategy. The study showed that (1) the impairment in the reference memory of TgCRND8 mice was reduced when a hidden platform was cued, and that (2) both working and reference memory systems of TgCRND8 mice, but not (3) the plasticity of choice between search strategies, are compromised by the transgene-induced pathology.     

Evidence for the substantia nigra pars compacta as an essential component of a memory system independent of the hippocampal memory system

The aim of the present study was to test if the nigrostriatal pathway is an essential component for a water maze cued task learning and if it works independently of the hippocampal memory system. This hypothesis was tested using an animal model of Parkinson's disease in which male Wistar rats were lesioned in the substantia nigra pars compacta (SNc) by the intranigral infusion of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), thus causing a partial depletion of striatal dopamine. SNc-lesioned and sham-operated animals were implanted bilaterally with guide cannulae above the dorsal hippocampus in order to be tested after the administration of 0.4 microl 2% lidocaine or saline into this structure. The animals were tested in a spatial or in a cued version of the water maze, memory tasks previously reported to model hippocampal-dependent spatial/relational and striatal-dependent S-R learning, respectively. Hippocampal inactivation, but not SNc lesion, impaired learning and memory in the spatial version of the water maze. An opposite situation was observed with the cued version. No significant interaction was observed between the SNc lesion and hippocampal inactivation conditions affecting scores in the spatial or in the cued version of the water maze. These results suggest that the nigrostriatal pathway is an essential part of the memory system that processes S-R learning and that it works independently of the hippocampal memory system that processes spatial/relational memories.     

樟柳碱所致学习和记忆障碍动物模型的探讨

本实验采用水迷津法、步下法和步入法,用樟柳碱制备学习和记忆障碍的动物模型。结果表明,樟柳碱(10毫克/公斤)可使小鼠引起学习障碍,其表现为通过水迷津全程的时间延长,而且小鼠进入盲路的错误次数明显增多。樟柳碱(10毫克/公斤)可使小鼠引起记忆障碍,在樟柳碱的影响下,小鼠步下平台或步入暗箱的潜伏期明显缩短,并使它们的错误次数增多。本实验结果进一步表明,测定小鼠的记忆障碍,步入法似乎比步下法更为敏感。     

Effects of ketamine on tunnel maze and water maze performance in the rat

The NMDA receptor, which has been implicated in memory formation, is noncompetitively blocked by ketamine. The present study examines the effect of ketamine (0, 3, 6, 12, and 25 mg/kg body wt; ip) on tunnel maze and water maze performance in Wistar rats. In the hexagonal tunnel maze (HTM) high doses of ketamine (12 and 25 mg/kg) decreased locomotor activity. Moreover, ketamine induced perimeter walking (6, 12, and 25 mg/kg) and attenuated exploratory efficiency (25 mg/kg). When the HTM was converted into a modified six-arm radial maze, ketamine impaired short-term but not long-term memory. In the Morris water maze, rats injected with ketamine (12 and 25 mg/kg) acquired a spatial navigation task more slowly than controls. When the escape platform was removed, the drug-treated rats did not preferentially search for it in the area where the platform had been during the acquisition phase. However, when the escape platform was visible, no differences in the performance of ketamine-treated and control rats could be found. In summary, ketamine seems to attenuate some but not all forms of learning in the tunnel maze and it impairs the acquisition of a spatial navigation task.     

Intraseptal Flumazenil Enhances, while Diazepam Binding Inhibitor Impairs, Performance in a Working Memory Task

GABA_A/benzodiazepine receptors in the medial septum modulate the activity of cholinergic neurons that innervate the hippocampus. Injection of benzodiazepine (BDZ) agonists into the medial septum impairs working memory performance and decreases high-affinity choline transport (HAChT) in the hippocampus. In contrast, intraseptal injection of the BDZ antagonist flumazenil increases HAChT and prevents the memory deficits induced by systemic BDZs. The present studies attempted to further characterize the behavioral effects of medial septal injections of flumazenil to an endogenous negative modulator of the GABA_A/BDZ receptor complex, diazepam binding inhibitor (DBI). Male Sprague–Dawley rats were cannulated to study the effects of intraseptal injections of these BDZ ligands on spatial working memory, anxiety-related behaviors in the elevated plus maze, and on general locomotor activity. Intraseptal flumazenil (10 nmol/0.5 μl) produced a delay-dependent enhancement of DNMTS performance after an 8-h, but not a 4-h, delay interval. This promnestic dose of flumazenil had no effect on locomotor activity and did not produce changes in measures of anxiety on the plus maze. Intraseptal injection of DBI had no effect (8 nmol/0.5 μl) or slightly impaired (4 nmol/0.5 μl) DNMTS radial maze performance following an 8-h delay, without producing changes in locomotion or plus maze behavior. These data demonstrate that flumazenil has a unique profile of activity in enhancing working memory following intraseptal injection.     

Amnesia produced by anisomycin in an appetitive task is not due to conditioned aversion

Two experiments investigated the effects of lithium chloride (LiCl) and anisomycin (ANI) in a water reward Y-maze task. In Experiment 1, male CD-1 mice given weak or strong training were injected post-training with either saline or LiCl (150 mg/kg), which has been reported to produce conditioned aversion in mice. One day after training, both LiCl groups avoided the rewarded arm of the maze and drank less water than saline-injected controls. Two days after training, the strongly trained LiCl mice showed avoidance, while both LiCl groups drank less water. In Experiment 2, weakly trained mice given pre- and post-training ANI (30 mg/kg) were amnesic on the second test day compared to mice that received post-trial saline. However, water consumption was increased on the test day for both groups. LiCl produced a different pattern of results than ANI in this task. On the basis of these results, it is suggested that amnesia produced by ANI is due to impaired memory formation and not to conditioned aversion.     

Higher levels of estradiol replacement correlate with better spatial memory in surgically menopausal young and middle-aged rats

The current study investigated whether, for spatial reference memory, age impacts (1) sensitivity to surgical ovarian hormone loss (Ovx), (2) response to estradiol therapy (ET), and (3) the relation between circulating estradiol levels and memory scores in ovary-intact sham and Ovx plus ET rats. Young, middle-aged and aged Fischer-344 rats received sham, Ovx or Ovx plus ET treatments, and were then tested on the Morris maze. After the last test trial, a probe trial was given whereby the platform was removed. Circulating estradiol levels were then determined and correlated with performance. In Study 1, Ovx facilitated learning on day one, but impaired performance after day one, in young rats. Ovx did not influence performance in middle-aged rats. In young and middle-aged Ovx rats, ET enhanced performance with higher exogenous estradiol levels correlating with better performance during testing and the probe trial. There was no relationship between endogenous estradiol levels and performance in sham young or middle-aged rats. Study 2 showed that, like middle-aged rats, aged rats were not impacted by Ovx. Further, for aged Ovx rats, the ET regimen that was beneficial at earlier ages was no longer effective during test trials, and had only minor benefits for platform localization as assessed by the probe trial. Collectively, the findings suggest that the effects of Ovx as well as responsivity to the currently utilized ET regimen changes with age. Further, there appears to be a distinction between sensitivity to Ovx and responsiveness to ET after Ovx for spatial reference memory performance.     

SLV330, a cannabinoid CB1 receptor antagonist,ameliorates deficits in the T-maze,object recognition and Social Recognition Tasks in rodents

Cannabinoid CB₁ receptor (CB₁R) signaling has been suggested to play an important role in the regulation of memory and cognition. In the present study, our aim was to investigate whether the CB₁R antagonist SLV330 (doses ranging from 0.3 to 10 mg/kg, given orally, p.o.) could ameliorate impairments in distinct aspects of cognition using different disruption models in both mice and rats. Effects of SLV330 were tested on working memory deficits in the T-maze Continuous Alternation Task (T-CAT) in mice; episodic memory deficits in the Object Recognition Task (ORT) and Social Recognition Task (SRT) in rats. The acetylcholinesterase inhibitor (AChEI) donepezil (Aricept®, approved for symptomatic treatment of Alzheimer’s disease) and nicotine were used as reference compounds.SLV330 markedly improved aging and scopolamine-induced memory deficits in the T-CAT in mice with a lowest effective dose (LED) of 1 mg/kg p.o., while reversing the cognitive dysfunction induced by the N-methyl-d-aspartate (NMDA) antagonist dizocilpine (MK-801) only at the middle dose of 3 mg/kg. In the ORT, we have found that combined administration of subthreshold doses of SLV330 (1 mg/kg, p.o.) and the AChEI donepezil (0.1 mg/kg, p.o.), that had no discernable effects on performance when given alone, enhanced memory performance in Wistar rats with deficits induced by the muscarinic antagonist scopolamine, suggestive of additive synergistic effects of SLV330 and donepezil on cognitive impairment. Finally, SLV330 was found to have cognition enhancing properties in a time delay paradigm in the SRT at a LED dose of 3 mg/kg (p.o.).In conclusion, the CB₁R antagonist SLV330 was found to clearly improve memory in several preclinical models for cognitive impairment.